SU1724141A1 - Nutritious medium for raising oak eggar moth - Google Patents

Abstract

This invention relates to agriculture, in particular to sericulture. In order to increase the survival and silk productivity of oak silkworm and caterpillars are fed on a nutrient medium containing, in wt.%: Pedunculate oak leaf powder 18.96-26.67, soybean meal powder 2.19-3.04, agar-agar 2.56 -3.34, glucose 2.50-3.33, ascorbic acid 2.46-3.33, cellulose powder 4.06-5.73, sorbic acid 0.07–0.10, retanol-acetate 0 , 05-0.07, riboflavin 0.04-0.08, thiamine bromide 0.03-0.06, nicotinamide 0.04-0.07, waste from gonad of squid or fish 0.25-0.35, distilled water else. 3 tab.

Description

The invention relates to agriculture, in particular to sericulture, and can be used in artificial breeding of oak silkworm at feeding stations during its year-round and seasonal cultivation, as well as in testing the biological and entomocidal activity of various microbiological and chemical preparations in laboratory conditions. x

The purpose of the invention is to increase the survival and silk productivity of oak silkworm.

Oak silkworm larvae during development are fed with nutrient medium containing dry oak leaf powder, soybean meal powder, agar-agar, carbohydrate and distilled water, as carbohydrate it contains glucose and additionally ascorbic acid, cellulose powder, sorbic acid, re - Tanol-acetzt, riboflavin, thiamine-bromide, nicotinamide and waste from the gonad of squid

or fish in the following ratio of components, May. %: Leaf powder

English oak - 18.96-26.67 Soybean powder

Meal2,19-3,04

Agar-Agar2.56-3.34

Glucose 2.50-3.33

Ascorbic acid2, 46-3.33 Cellulose powder in 4.06-5.73 Sorbic acid 0.07-0.10 Retanol-acetate 0.05-0.07 Riboflavin 0.04-0.08 Thiamine-bromide 0.03-0 , 06 Nicotinamide 0.04-0.07 Waste from the gonad

squid or fish 0,25-0,35

Distilled water Else Waste from gonad of squid or fish contains 4.12% on dry matter of phosphorus, 2.69% of calcium, 0.43% of potassium, 0.57% of sodium,

Xi

BO

J

the protein part of the waste is 53.01% and it includes, in terms of mmol per 1 g dry weight: lysine 356.21; histidine 76.99; arginine 122.44; asparagine 358.60; threonine 183.90; serine 220.92; glutamine 429.88; proline 182.98; glycine 329.74; alanine 292.00; cysteine29,27; valine 189.12; methionine 36.57; isoleucine 161.91; leucine 265.83; tyrosine 97.43; phenylalanine 115.40; the lipid part of the mixture, comprising 2.89% dry weight, contains phospholipids (lecithin, phosphatidyl-ethanolamine, sphingomyelin) 1.45%, cholesterol 0.43%, free fatty acids 0.86%, triglycerolO ,15.

Example. The effectiveness of the nutrient medium was studied on the oak silkworm of the de monovoltine breed Polessky tassar.

Nutrient medium for growing oak silkworm was prepared as follows.

Freshly harvested oak leaves were harvested in July and dried in a conventional manner in the attic or under a canopy with good ventilation, folding the sheet in three layers on paper. The dry leaf was stored for up to one year in closed paper bags in a dry, unheated and well-ventilated area. Before preparing the nutrient medium, the dry leaves were ground with an electric mill until a uniform fine grinding of the powder was obtained. Powder from dry leaves of pedunculate oak according to the options in the amounts indicated in the table. 1, mixed with soybean meal powder, glucose, cellulose powder, sorbic acid, waste from squid or fish gonads, cooled to + 60 ° C with an aqueous solution of agar-agar, a solution of ascorbic acid, retanol acetate, riboflavin, thiamine bromide and nicotinamide. The remaining portion of distilled water was added to the resulting feed mixture and thoroughly mixed again to obtain a uniform consistency. In order to obtain feed plates, cooled (up to + 10 ° C) nutrient medium was rolled out between plastic wrap. The resulting feed plates, with a thickness of up to 1.0 mm, were rolled into polyethylene rolls and stored in a refrigerator at + 5 ° C for no more than ten days.

When preparing the nutrient medium, waste from gonad of squid or fish contains 4.12% of dry matter of phosphorus, 2.69% of calcium, 0.43% of potassium, 0.57% of sodium, 53.01% of the protein content and included in the calculation mmol per 1 g dry weight: lysine 356.21; histidine 76.99; arginine 122.44; asparagine 358.60; threonine 183.90; serine 220.92; glutamine 429.88; proline 182.98; methionine 36.57; isoleucine 161.91; leucine 265.83; tyrosine 97.43; phenylalanine 115.40; the lipid part of the waste, constituting 2.89% of dry mass, contains phospholipids (lecithin, phosphatidyl-ethanolamine; sphingomyelin) 1.55%, cholesterol 0.43%, free fatty acids 0.86, triglycerol 0.15%.

Until the end of the third age, the caterpillars of the oak silkworm were raised in Petri dishes, and starting from the fourth, in cardboard or wooden boxes, the upper part of which is covered with plastic wrap. The medium in the form of sliced slices was placed on litter paper (such as filtered) along the walls. The litter, excrement and uneaten food were removed after 2 days. When caring for the caterpillars, the general recommendations reflected in the technology for growing oak silkworm and monovoltine breed Polessky tassar under industrial conditions were taken into account (Moroz N. S., Aretinskaya TB, Vititnev I.V. and others. Recommendations on feeding oak silkopr yes Polessky Tassar in industrial conditions. Ukrainian Research Institute of Scientific and Technical Information and Technical and Economic Research of the State Planning Committee of the Ukrainian SSR, Kiev Branch No. 4270-88- 304-110, Kiev, 1988. -5c).

Caterpillars of variants according to a known method were grown on an artificial nutrient medium, which included dry oak leaves 6.0 g; dried soy bean powder 1.0 g; sucrose 1.0 g; agar-agar 1.0 g and antiseptic solution (2 g of Na-dehydroacetate in 1 liter of water) 27-21 ml, respectively, for caterpillars from the first to the third and from the fourth to the end of the fifth ages. On the described artificial food, oak silkworm caterpillars were grown in Petri dishes until the end of the third age. Starting from the fourth age, the caterpillars were fed in wooden boxes in a special room, while maintaining a humidity of 70-80% and an air temperature of + 26 ° C. The feed fed twice a day was cut into slices 0.1–1.0 mm thick and fed to the caterpillars 1.

In tab. Table 2 presents data on the effect of artificial nutrient medium on the survival and productivity of oak silkworm. The analysis of the digital material shows that in insects grown on the proposed artificial environment, the best indicators on the survival rate of caterpillars and the number of curled cocoons are obtained

in variants B, C, and G. In these variants, compared with the variant by a known method, the death of the caterpillars of the oak and silkworm decreased by 20.42; 17.18 and 16.75% and the number of cocoons increased by 35.78; 31.19 and 29.36%, respectively.

As can be seen from the data presented in table. 3, the oak silkworm, bred on the proposed nutrient medium, with both boundary and average amounts of the components that make up the medium, is greater in silk production than insects raised in similar conditions on the nutrient medium of the variant by a known method. The highest mass indices of the cocoon, pupa, and silk shell were obtained in variants B, C, and G. A further increase in the number of components of the nutrient medium and a decrease in the water content in it, as shown in variant D, contributes to a decrease in silk productivity and is impractical.

An analysis of experimental studies shows that in all the studied parameters, the experimental variants significantly exceed the indicators of the variants by a known method. The nutrient medium for oak silkworm offered as feed is simple in composition and does not require special sophisticated cooking equipment. Due to the application of the proposed nutrient medium, the biological indicators of the oak silkworm are improved, the survival rate increases, the number of curled cocoons increases, and the silk productivity increases during year-round or seasonal cultivation under artificial conditions, i.e., a new positive effect is achieved.

Experimental and production tests of the nutrient medium for the cultivation of oak silkworm, carried out on an experimental basis for the cultivation of the oak silkworm at the Kamensky forest area of the Glyboksky inter-farm forestry enterprise Chernivtsi Oblagroprom, showed that the use of the nutrient medium allows for the growth and development of insects

to increase the survival and silk productivity of the oak silkworm and the Polesye tarsar.

Thus, as a result of the use of a nutrient medium for growing oak silkworm, a decrease in the development time of larvae in the larval stage by 8.33% and an increase in survival by 23.03% was noted.

As a result of the application of the proposed nutrient medium, it was possible to increase the yield of benign cocoons by 19.27%, and the weight of the silk shell per kilogram per kilogram by 43.85% compared to the control variant.

Claims (1)

Claims Nutrient medium for growing oak silkworm containing dry oak leaf powder, soybean meal powder, agar-agar, carbon and distilled water, in order to increase the survival and silk productivity of oak silkworm as carbohydrate contains glucose and additionally ascorbic acid, cellulose powder, sorbic acid, retanol acetate, riboflavin, thiamine bromide, nicotinamide, and waste from squid or fish gonads in the following ratio, wt.%: By leaf powder English oak18,96-26,67 Soybean powder Meal2,19-3,04 Agar-Agar2.56-3.34 Glucose 2.50-3.33 Ascorbic acid2, 46-3.33 Cellulose powder 4.06-5.73 Sorbic acid 0.07-0.10 Retanol-acetate0.05-0.07 Riboflavin0.04-0.08 Thiamine bromide 0.03-0 , 06 Nicotinamide 0.07-0.04 Waste from the gonad squid or fish 0,25-0,35 Distilled water Else Table 1 The content of the components in the nutrient medium used for feeding the oak silkworm caterpillars of experimental options table 2 Effect of artificial nutrient medium on survival and productivity of oak silkworm yes Effect of artificial nutrient medium on the silk productivity of oak silkworm Table 3