SU1720597A1 - Method for preparation of regenerated plants to be planted in non-sterile conditions - Google Patents

Abstract

The invention relates to biotechnology, in particular, to the culture of isolated tissues and organs, and can be used in the process of transplanting plants in non-sterile conditions. The aim of the invention is to increase the survival rate of plants under non-sterile conditions and reduce their infection. The goal is achieved by the fact that plants are acclimatized at the plant rooting stage. The coating is removed from the cultivation vessels, placed in a horizontal position on a roller and rotated in a vertical plane for 10-14 days, and before planting in the substrate of the plant, the regenerates are kept in water for 24 hours. Plants are regenerated with 11–15 leaves to be planted into the substrate. The method allows plants to take root from 60 to 100%, while decreasing the infected ™. 1 hp f-ly, 4 tab. fe

Description

The invention relates to the field of culture of isolated tissues and organs and can be used in the process of transplanting plants in non-sterile conditions.

There is a known method of growing plants when the cultivation vessels are fixed in a roller and rotated in a vertical plane. However, this method does not allow the plant to be adapted to non-sterile conditions.

The closest technical solution to the present invention is a method of transplanting regenerated plants under non-sterile conditions, in which plants are acclimatized to non-sterile conditions directly in

the cultivation vessel by removing the coating from the vessel.

However, this known method has the following disadvantages. The vertical arrangement of the cultivation vessel, from which the coating is removed, leads to the ingress of microorganisms onto the surface of the nutrient medium and infection of the environment and plants, which impairs their survival.

The purpose of the invention is to increase the survival rate of plants in a non-sterile substrate and reduce their infected ™.

The goal is achieved by removing the coatings from the cultivation vessels at the rooting stage, the vessels are fixed horizontally in a roller and rotated in

VI

vertical plane for 10-14 days. After rotation, the plants are removed from the vessels and kept in water for 24 hours, after which they are planted in the substrate. Regenerates with 11–15 leaves are selected for planting into the substrate.

A comparative analysis of the proposed solution with the well-known one shows that the proposed method differs from the well-known one in that the vessels with the removed coatings rotate in a roller in a vertical plane for 10-14 days. The horizontal arrangement of vessels prevents infection of the nutrient medium and plants. The rotation of the vessels in the vertical plane ensures the growth of plants in height (in the direction out of the vessel) due to the uniform influence of the earth's gravitational forces on all organs of the plant and contributes to their better acclimatization to non-sterile conditions.

Namachivaniya taken out of the vessels of plants in the water allows plants to compensate for water loss as a result of the transpiration process, which also increases the survivability of plants.

When planting in the substrate, plants with 11-15 leaves are used. Plants with fewer leaves are weakened, so their survival rate decreases. The survival rate of plants with more than 16 leaves is reduced due to their higher transpiration.

Example. Prior to acclimatization, blackberry plants are grown on rooting nutrient based on the recipe Murashige-Skoog (1962) and diluted twice. The composition of the medium, mg / l:

Ammonium nitrate 825.0

Potassium nitrate 950,0

Calcium chloride 220.0

Magnesium sulfate 185.0

Potassium phosphate monosubstituted 85.0

Iron sulfate 13,6

Ethylene diamine tetraacetate sodium 18.7

Boric acid 3.1

Manganese sulfate 11.2

Sulfuric acid cycle 4,3

Potassium iodide 0.42

Sodium molybdate 0,13

Copper sulfate 0.013

Cobalt chloride 0,013

Myoinositis 50.0

Thiamine 0.25

Pyridoxine 0.25

Nicotinic acid 0,25

Ascorbic acid 0.5

Indolylmasl on acid 1.0

Sucrose 15,000

Agar 7000

Water Up to 1 l

After the rooting of the plants, the coatings are removed from the cultivation vessels, the vessels are horizontally secured in a roller and rotated in a vertical plane for 10-14 days. A rotation period of less than 10 days does not ensure full acclimatization of plants (Table 1). Rotation within 10-14 days gives 100% survival of plants. An increase in the duration of rotation over 14 days is impractical, since this leads to a lengthening of the technological cycle of the production of planting material and drying of the nutrient medium, which is an unfavorable factor for the development of plants.

After rotation, the regenerative plants are removed from the nutrient medium, kept in water for 24 hours, and planted in the substrate.

The proposed method reduces the infection of plants (Table 2) and increases their survival rate both when landing in perlite and in the peat-sand mixture (Tables 2 and 3).

For planting in the substrate, plants are regenerated with 11-15 leaves. The effect of the number of leaves on the survival rate of blackberry plants is given in Table. four.

Thus, the proposed method of preparing regenerate plants for planting in non-sterile conditions reduces the infection of plants, increases their survival by 1.7-1.9 times.

In the production of planting material in the amount of 1000 plants per year, the economic effect of using the invention reaches 4,000 rubles. savings compared with the known method.

Claims (2)

1. A method of preparing regenerated plants for planting in non-sterile conditions, including acclimatizing plants to environmental conditions by removing the coating from a cultivation vessel, characterized in that, in order to increase the survival rate of plants in non-sterile conditions and reduce their infection, acclimatize plants are carried out at the plant rooting stage, after removal of the cover, the cultivation vessel is placed in a horizontal position on a roller and rotated in a vertical plane for 10-14 days, and then Eni-regenerates incubated for 24 hours in water. 2. A method according to claim 1, characterized in that plants are regenerated with 11 to 15 leaves for planting in the substrate. Table 1 Table 3 Table 4