SU1712418A1 - Nutrient medium for synthesis of @@@-carotene - Google Patents

Abstract

This invention relates to the microbiological industry and concerns the production of vitamins, in particular (3-carotene). The aim of the invention is to increase the degree of isomeric count of the target product. This is ensured by the fact that the nutrient medium for the biosynthesis of carotene, containing oil-containing components selected from the row: vegetable oil, soap stock, phosphatide emulsion, and water, additionally contains waste of sunflower seed purification, in the following ratio of components, wt, %: waste from cleaning sunflower seeds 3.0-7.0; oil-containing components selected from the series: vegetable oil, soap stock, phosphatide emulsion 2.0-6.0, water the rest. 2 tab. •

Description

(L

with

In the amount of 20% of the volume of the nutrient medium, component, wt.%:

Cleaning waste

sunflower3.0

Vegetable oil 6.0

Water91.0

and grown under aerobic conditions until jS-carotene accumulates 49937 µg / 100 ml of culture fluid for 120 hours.

The biomass amount is 3.0 g / 100 ml of a.s.s., biomass productivity is 16646 µg / ha.s, or 16.6 g / kg, degree of isomeric purity of 8-carotene is 97.5%.

PRI mme R 2. The process is conducted according to example 1.

The composition of the nutrient medium, wt.%:

Cleaning waste

sunflower4.0

Vegetable oil 4.0

Water .92.0

The accumulation of D-carotene is 96117 µg / 100 ml, the amount of biomass is 4.7 g / 100 ml of al.s.beet, the productivity of biomass is 20,450 µg / ha.s.w. or 20.5 g / kg, isomeric purity / 3-carotene, 97.8%. ,

PRI me R 3. The process is conducted according to example 1.

The composition of the nutrient medium, wt.%:

Cleaning waste

sunflower7.0,

Vegetable oil 2.0

Water91.0

The accumulation of jS-carotene 68321 µg / 100 ml, the amount of biomass is 6.5 g / 100 ml of a.s.w., the productivity of biomass is 12,422 µg / ha.s.w. or 12.4 g / kg, isomeric purity / 5-carotene 98.9%.

PRI me R 4. The process is conducted according to example 1.

The composition of the nutrient medium, wt.%:

Waste scrubbing seeds

sunflower 4.0

Phosphatide emulsion 4.0

Water 92.0

Accumulation of D-carotene. 112702 µg / 100

ml, the amount of biomass is 4.9 g / 100 ml of a.s.s.

biomass productivity of 23,000 mcg / ha.s.v.

or 23.0 g / kg, isomeric purity

carotene 97.8%.

PRI me R 5. The process is conducted according to example 1.

The composition of the nutrient medium, wt.%:

Cleaning waste

sunflower 4,0

Soapstock4.0

Water 92.0

The accumulation of D-carotene 105546 µg / 100 ml, the amount of biomass of 4.3 g / 200 ml. Asv.w., biomass productivity 24545 µg / ha.s.v.

or 24.5 g / kg, the degree of isomeric purity Lkarotina97,8%.

PRI me R 6. The process is conducted according to example 1.

5The composition of the nutrient medium, wt.%:

Waste from the cleaning of sunflower seeds6.0

Phosphatide emulsion 4,0 Water 90,0

0 Accumulation / W-carotene 135844 µg / 100 ml, the amount of biomass is 5.2 g / 100 ml.a.s.w., biomass productivity is 26124 μg / g.s.v., or 26.1 g / kg, isomeric degree purity / J-carotene 98.7%.

5 Limer 7. The process is conducted according to an example.

one.

The composition of the nutrient medium, wt.%:

Cleaning waste

sunflower .7,0

0 vegetable oil 2.0

Water91.0

The accumulation of / 5-carotene 68312 mcg / 100 ml, the amount of biomass 5.5 g / 100 ml. A. c.in., the productivity of biomass 12420 mcg / g and.s.w. 5 or 12.4 g / kg, degree of isomeric purity 99.1%.

PRI me R 8. The process is conducted according to example 1.

The composition of the nutrient medium, wt.%: 0 Waste cleaning seeds

sunflower7.0

Phosphatide emulsion 2.0

Water91.0

Accumulation of 3-carotene 71925 µg / 100

5 ml, the amount of biomass is 6.0 g / 100 ml of a.s.s.

biomass productivity 11987 mkg / g a.s.s.v.

or 12.0 g / kg, isomeric purity

/ 5 carotene 99,0%.

PRI me R 9. The process is conducted according to example 0 1.

The composition of the nutrient medium, wt;%:

Cleaning waste

sunflower7.0

Soapstock2.0

5Water91.0

Accumulation of / S-carotene 67832 µg / 100

ml, the amount of biomass is 5.0 g / 100 ml of a.s.s.

biomass productivity 13566 mcg / g as.s.v.

or 13.6 g / kg, isomeric purity

0 D-carotene 98.9%.

Example 10. The process is conducted according to the example.

one.

The composition of the nutrient medium, wt.%: Waste cleaning seeds 5 sunflower 3.0

Vegetable oil 6.0 Water, 91.0

Accumulation of 3-carotene 49937 µg / 100

ml, the amount of biomass 3.0 g / 100 ml.a.s.v ..

biomass productivity is 16645 µg / g a.s.s. or 16i6 g / kg degree of isomeric purity) of carotene 97.5%.

EXAMPLE The process is conducted according to example 1.

The composition of the nutrient medium, wt.%;

Waste cleaning seeds,

sunflower3.0

Phosphatide emulsion 6.0

Water91.0

Accumulation / Z-carotene 54856 µg / 100 ml, the amount of biomass 3.5 g / 100 ml a, q, B., the productivity of biomass 15673 µg / g Al.s. or 15.6 g / kg, isomeric purity / S-carotene 97.4%.

Example 12. The process is carried out according to example 1.

The composition of the nutrient medium, wt.%:

Cleaning waste

sunflower3.0

CoariCTOK6,0,

Water91.0

The accumulation of D-carotene 45615 µg / 100 ml, the amount of biomass is 2.7 g / 100 ml of al.s.beet.in, the productivity of biomass is 16894 µg / ga.s.s. or 16.9 g / kg, isomeric purity) of 8-carotene, 97.6%.

PRI me R 13. The process is conducted according to example 1.

The composition of the nutrient medium, wt.%:

Cleaning waste

sunflower2.0

Phosphatide emulsion 4.0

Water94.0

The accumulation of / 3-carotene 30857 µg / 100 ml, the amount of biomass 3.8 g / 100 ml, biomass productivity of 8120 µg / g as.v. or 8.1 g / kg, degree of isomeric purity) of carotene 96.8%.

PRI me R 14. The process is conducted according to example 1.

The composition of the nutrient medium, May,%:

Cleaning waste

sunflower; 8.0

Phosphatide emulsion 4.0

Voda88,0

The accumulation of / S-carotene is 29754 ml, the amount of biomass is 6.9 g / 100 ml of rpb, the productivity of biomass is 4312 µg / g as.v. or 4.3 g / kg, the degree of isomeric purity of carotene is 96.7%.

The compositions and properties of nutrient media in examples 1-14 are given in figd.1.

With the introduction into the nutrient medium of waste of cleaning sunflower seeds in the amount of 3.0-7.0% (examples 1-12), the activity of the obtained culture liquid is at the level of 45615-135844 µg / 100 ml. At the same time, the biomass productivity (12.026, 1 μg / g) meets the requirements of the technical conditions for the feed preparation of microbial carotene (not less than 10 g / kg / carotene).

The degree of isomeric purity of β-carotene in the biomass containing waste of sunflower seeds is 97.4-99.1%.

High biomass productivity and high degree of isomeric purity of the target product () of 8-carotene allow to obtain crystalline / carotene corresponding to the world standard (mass fraction of b-carotene - isomeric purity of 99.9%, 182-184 ° С) .

With the introduction of 2.0 and 8.0% of the waste of sunflower seed cleaning (examples 13 and 14) into the composition of the medium, the productivity of biomass decreases and is at the level of 4.3-8.1 g / kg.

Table 2 presents comparative data on the main characteristics of the biomass obtained from the proposed and known nutrient media. Biomass, both proposed and known, was grown under conditions.

Thus, the proposed nutrient medium provides for obtaining biomass with a higher degree of isomeric purity / 3-carotene (by 1-2%) while maintaining its activity.

Claims (1)

Invention Formula Nutrient medium for biosynthesis / 3-carotene containing oil-containing components selected from the series: vegetable oil, soap stock, Phosphatide emulsion, si, and water, characterized in that, in order to increase the isomeric purity of the target product, it additionally contains waste of cleaning sunflower seeds in the following ratio of components, wt.%: Waste of cleaning sunflower seeds 3,0-7,0 Oil-containing components selected from the series: vegetable oil, soap stock, Phosphatide. i emulsion: 2.0-6.0 WaterEverything Table I