SU1707535A1 - Method of determination of elasticity of erythrocytes - Google Patents
Method of determination of elasticity of erythrocytes Download PDFInfo
- Publication number
- SU1707535A1 SU1707535A1 SU894787079A SU4787079A SU1707535A1 SU 1707535 A1 SU1707535 A1 SU 1707535A1 SU 894787079 A SU894787079 A SU 894787079A SU 4787079 A SU4787079 A SU 4787079A SU 1707535 A1 SU1707535 A1 SU 1707535A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- elasticity
- erythrocytes
- determination
- samples
- blood samples
- Prior art date
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Description
создаетс напр жение сдвига.пор дка 200 дин/см2, через 20 с в зазор между цилиндрами введен 1,0 мл 0,8%-ного раствора глютаральдегида, через 10 с вращение наружного цилиндра остановлено . Суспензи эритроцитов помещена в поле зрени светового микроскопа. Подсчитано процентное содержание деформированных (выт нутых) эритроцитов Оно равно 80%.shear stress is created. about 200 dyn / cm2, after 20 s 1.0 ml of 0.8% glutaraldehyde solution is introduced into the gap between the cylinders, and after 10 s the rotation of the outer cylinder is stopped. The erythrocyte suspension is placed in the field of view of the light microscope. The percentage of deformed (elongated) erythrocytes is calculated. It is 80%.
Втора проба: 1,5 мл оставиейс суспензии этих же эритроцитов помещают в зазор между двум коаксиальными цилиндрами. Через 20 с после вращени наружного цилиндра со скоростью 4400 об/мин в зазор между цилиндрами введен 1,0 мл 0,1%-ного раствора глютаральдегида . После прекращени вращени наружного цилиндра суспензию эритроцитов помещают в поле зрени светового микроскопа и подсчитывают процентное содержание выт нутых эритроцитов . Оно равно 43%. Т.е. в исходное состо ние вернулось 80 - 43 37%Second sample: 1.5 ml of the remaining red blood cell suspension is placed in the gap between two coaxial cylinders. 20 seconds after the outer cylinder rotates at a speed of 4400 rpm, 1.0 ml of a 0.1% glutaraldehyde solution is introduced into the gap between the cylinders. After the rotation of the outer cylinder is stopped, the erythrocyte suspension is placed in the field of view of the light microscope and the percentage of erythrocytes is calculated. It is 43%. Those. 80 - 43 37% returned to its original state
упругость равна 37%, она определ етс как достаточна .elasticity is 37%; it is defined as sufficient.
В предлагаемом способе по сравнению с известным происходит сокращение времени исследовани в 70 раз.In the proposed method, in comparison with the known, the study time is reduced by a factor of 70.
Упрощение способа достигаетс за счет отсутстви необходимости использовани чрезвычайно сложной и дорогосто щей микрофотометрлческой техники.Simplification of the method is achieved due to the absence of the need to use extremely complex and expensive microphotometric techniques.
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU894787079A SU1707535A1 (en) | 1989-11-27 | 1989-11-27 | Method of determination of elasticity of erythrocytes |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU894787079A SU1707535A1 (en) | 1989-11-27 | 1989-11-27 | Method of determination of elasticity of erythrocytes |
Publications (1)
Publication Number | Publication Date |
---|---|
SU1707535A1 true SU1707535A1 (en) | 1992-01-23 |
Family
ID=21493950
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SU894787079A SU1707535A1 (en) | 1989-11-27 | 1989-11-27 | Method of determination of elasticity of erythrocytes |
Country Status (1)
Country | Link |
---|---|
SU (1) | SU1707535A1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU2585113C2 (en) * | 2014-06-26 | 2016-05-27 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Московский государственный университет имени М.В. Ломоносова" (МГУ) | Method of measuring erythrocyte deformability distribution parameters |
-
1989
- 1989-11-27 SU SU894787079A patent/SU1707535A1/en active
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU2585113C2 (en) * | 2014-06-26 | 2016-05-27 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Московский государственный университет имени М.В. Ломоносова" (МГУ) | Method of measuring erythrocyte deformability distribution parameters |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Wanson et al. | Rabbit skeletal muscle glycogen: a morphological and biochemical study of glycogen ß-particles isolated by the precipitation-centrifugation method | |
Shiga et al. | The decreased membrane fluidity of in vivo aged, human erythrocytes. A spin label study | |
Nash et al. | Mechanical properties of oxygenated red blood cells in sickle cell (HbSS) disease | |
Hoogeveen et al. | Water-soluble proteins of the human red cell membrane | |
Reinhart et al. | Albumin affects erythrocyte aggregation and sedimentation | |
US5459073A (en) | Method and composition for preserving antigens and process for utilizing cytological material produced by same | |
US3574137A (en) | Multiple-analysis hematology control comprising human red blood cells and fowl red blood cells in an anticoagulant containing human serologically compatible plasma medium | |
AU4411889A (en) | Stabilized lyophilized mammalian cells and method of making same | |
Bjerrum | Hemoglobin-depleted human erythrocyte ghosts: characterization of morphology and transport functions | |
JP4790818B2 (en) | Storage-stable cellular whole blood composition comprising elevated amounts of D-dimer | |
Skutelsky et al. | Distribution of the T-antigen on erythroid cell surfaces. Studies with peanut agglutinin, an anti-T specific lectin | |
JPH0726955B2 (en) | Method for preparing preserved whole blood sample | |
Fineschi et al. | Histological criteria for diagnosis of amanita phalloides poisoning | |
US5089415A (en) | Method for clotting heparized blood by the addition of protamine, thrombin and snake venom | |
Kido et al. | Macromolecular components of the vitelline membrane of hen's egg I. Membrane structure and its deterioration with age | |
SU1707535A1 (en) | Method of determination of elasticity of erythrocytes | |
Colten et al. | Purification of the first component of complement by zonal ultracentrifugation | |
EVANS et al. | A circulating factor (s) mediates cell depolarization in hemorrhagic shock | |
Wright et al. | Acute Babesia bovis infection: A study of the vascular lesions in kidney and lung | |
Durliat et al. | Coagulation in the crayfish, Astacus leptodactylus: attempts to identify a fibrinogen-like factor in the hemolymph | |
Day et al. | Report of the working party on platelets | |
Skaug | Lipoproteins in fluid from non‐keratinizing jaw cysts | |
Waldron-Edward et al. | Studies on Human Gastric Gel Mucin: Isolation and characterization of a major glycoprotein component | |
Lipovac et al. | Influence of lactate on the insulin action on red blood cell filterability | |
Rother et al. | Deviated lysis: lysis of unsensitized cells by complement. V. Generation of the activity by low pH or low ionic strength |