SU1707535A1 - Method of determination of elasticity of erythrocytes - Google Patents

Method of determination of elasticity of erythrocytes Download PDF

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Publication number
SU1707535A1
SU1707535A1 SU894787079A SU4787079A SU1707535A1 SU 1707535 A1 SU1707535 A1 SU 1707535A1 SU 894787079 A SU894787079 A SU 894787079A SU 4787079 A SU4787079 A SU 4787079A SU 1707535 A1 SU1707535 A1 SU 1707535A1
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USSR - Soviet Union
Prior art keywords
elasticity
erythrocytes
determination
samples
blood samples
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SU894787079A
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Russian (ru)
Inventor
Григорий Яковлевич Левин
Надежда Павловна Котяева
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Нижегородский научно-исследовательский институт травматологии и ортопедии
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Priority to SU894787079A priority Critical patent/SU1707535A1/en
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Description

создаетс  напр жение сдвига.пор дка 200 дин/см2, через 20 с в зазор между цилиндрами введен 1,0 мл 0,8%-ного раствора глютаральдегида, через 10 с вращение наружного цилиндра остановлено . Суспензи  эритроцитов помещена в поле зрени  светового микроскопа. Подсчитано процентное содержание деформированных (выт нутых) эритроцитов Оно равно 80%.shear stress is created. about 200 dyn / cm2, after 20 s 1.0 ml of 0.8% glutaraldehyde solution is introduced into the gap between the cylinders, and after 10 s the rotation of the outer cylinder is stopped. The erythrocyte suspension is placed in the field of view of the light microscope. The percentage of deformed (elongated) erythrocytes is calculated. It is 80%.

Втора  проба: 1,5 мл оставиейс  суспензии этих же эритроцитов помещают в зазор между двум  коаксиальными цилиндрами. Через 20 с после вращени  наружного цилиндра со скоростью 4400 об/мин в зазор между цилиндрами введен 1,0 мл 0,1%-ного раствора глютаральдегида . После прекращени  вращени  наружного цилиндра суспензию эритроцитов помещают в поле зрени  светового микроскопа и подсчитывают процентное содержание выт нутых эритроцитов . Оно равно 43%. Т.е. в исходное состо ние вернулось 80 - 43 37%Second sample: 1.5 ml of the remaining red blood cell suspension is placed in the gap between two coaxial cylinders. 20 seconds after the outer cylinder rotates at a speed of 4400 rpm, 1.0 ml of a 0.1% glutaraldehyde solution is introduced into the gap between the cylinders. After the rotation of the outer cylinder is stopped, the erythrocyte suspension is placed in the field of view of the light microscope and the percentage of erythrocytes is calculated. It is 43%. Those. 80 - 43 37% returned to its original state

упругость равна 37%, она определ етс  как достаточна .elasticity is 37%; it is defined as sufficient.

В предлагаемом способе по сравнению с известным происходит сокращение времени исследовани  в 70 раз.In the proposed method, in comparison with the known, the study time is reduced by a factor of 70.

Упрощение способа достигаетс  за счет отсутстви  необходимости использовани  чрезвычайно сложной и дорогосто щей микрофотометрлческой техники.Simplification of the method is achieved due to the absence of the need to use extremely complex and expensive microphotometric techniques.

Claims (1)

Формула изобретени  Способ определени  упругости эритроцитов путем помещени  проб крови в искусственный сдвиговый поток, отличающийс  тем, что, с целью упрощени  и ускорени  способа, исследуютс  две пробп крови, которые фиксируютс  после сн ти  сдвигового напр жени  растворами глютаральдегида в концентрации 0,8 и 0,1%, затем подсчитывают содержание деформированных эритроцитов в обеих пробах и по разнице между ними определ ют упругость эритроцитоп.The invention of the method for determining the elasticity of red blood cells by placing blood samples in an artificial shear flow, characterized in that, in order to simplify and speed up the method, two blood samples are examined, which are fixed after removing the shear stress with glutaraldehyde solutions at a concentration of 0.8 and 0, 1%, then calculate the content of the deformed erythrocytes in both samples, and the elasticity of the erythrocytope is determined from the difference between them.
SU894787079A 1989-11-27 1989-11-27 Method of determination of elasticity of erythrocytes SU1707535A1 (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2585113C2 (en) * 2014-06-26 2016-05-27 Федеральное государственное бюджетное образовательное учреждение высшего образования "Московский государственный университет имени М.В. Ломоносова" (МГУ) Method of measuring erythrocyte deformability distribution parameters

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2585113C2 (en) * 2014-06-26 2016-05-27 Федеральное государственное бюджетное образовательное учреждение высшего образования "Московский государственный университет имени М.В. Ломоносова" (МГУ) Method of measuring erythrocyte deformability distribution parameters

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