SU1707064A1 - Determination of concentration of biologically active substances - Google Patents

Abstract

The invention relates to the study of biologically active substances, to devices for determining their concentration using a test culture of microorganisms. The purpose of the invention is to improve the determination accuracy by more uniform growth of the test culture. The device contains a container 1 with a bottom 2 and a lid 3 into which gel 4c is poured with a test culture serving as a growth medium, and tubes b and rods 8 are placed in it. -culture. The tubes b are equipped with a measuring scale 10. The test substance 11 is placed in the tubes b on the gel 4, cultivated and measured on a scale of 10 of the microorganism growth zone. The device, in addition to improving the accuracy of determination, allows to speed up the analysis of substances. 3 il. / 3. (Ls

Description

77-70

6

,eight

vj

O 4 o

ABOUT

N

The invention relates to the study of biologically active substances, to devices for determining their concentration using microorganisms as a test culture.

The purpose of the invention is to improve the accuracy of determination by more uniform growth of the test culture of microorganisms.

FIG. 1 shows a device, a longitudinal section; in fig. 2 - the same at work; in fig. 3 - the same, with anaerobic test-culture of microorganisms,

The device contains a container 1 with a bottom 2 and a cover 3. In the container 1 a gel 4 is placed that serves as a medium for the growth of microorganisms, their test culture 5 (Fig. 2 and 3). In the container 1 in the gel 4, vertical graduated translucent tubes 6 are installed with the formation of a gap 7 between their lower edges and the bottom 2 of the container 1 for filling the tubes 6 with gel. The rods 8 are placed inside the tubes 6 and installed with the possibility of their being removed to form cavities 9 inside the tubes 6 inside the tubes 6 for supplying air to the test culture of 5 microorganisms. In addition, the tubes 6 have a measuring scale of 10, and the biologically active substance 11 is placed in the tubes 6 on the surface of the gel 4.

The device works as follows.

The sterilized container 1 with tubes 6 is filled with gel 4 - agar medium with test culture 5 at a temperature. Medium - gel 4 fills all tubes to the same level.

For anaerobic test culture 5, after gel freezing 4, the solution of active biologically active substance 11 is poured directly into tubes 6 on the layer of agar medium of gel 4, and for aerobic test culture 5 the test solution is poured into tubes 6 with rods 8. Then diffusion occurs biologically active substances. After that, the tank Tc with anaerobic test culture 5 is placed in a thermostat for cultivating microorganisms. In the case of an aerobic test culture 5, the residual liquid is first drained from the tubes 6 (turning over the container 1 with the lid 3 closed) and the surface of the gel 4 is dried a little. Then the rods 8 are removed and the container 1 is also placed in a thermostat.

After cultivation in a thermostat, the container 1 is removed and started to measure. To do this, remove the tube 6 from the tank 1 with tweezers. The growth zones of the test culture 5, or their absence, are recorded on a scale of 10 tubes 6. The size of the zones of standardized solutions is used to build a calibration curve and determine the concentration of the test solution of the biologically active substance.

In the case of an aerobic test culture 5 growth

or the absence of growth zones can be observed on the surface of the gel 4 after removing the rod 8. After removing the results, the container 1 and the tubes 6 are cleaned of the gel 4 (it is pushed out of the tubes with a glass rod) and sterilized.

The proposed device allows to significantly increase the accuracy of determining the concentration due to the uniform thickness of the gel 4. Diffusion & upright

downward direction is more efficient, which increases the resolution of measuring the growth zones or the lack of them on a scale of 10 tubes 6. Obtaining results in the form of volume (in milliliters) is more accurate and

more convenient than measuring the diameter of the circles of growth zones. The device is easier to sterilize with hot air and is easy to maintain sterile testing conditions.

The device greatly simplifies all the work in determining the concentration and preparation of sgo for work. It is much smaller and lighter, although it has the ability to receive the same number of measurements. 06slide measurement leads to time savings. A device approximately 5 times less requires an agar medium with a test culture of a microorganism.

Claims (1)

Formula of inventions A device for microbiological determination of the concentration of biologically active substances, containing a container with a bottom and a lid and a gel placed in the container that serves as a medium for the growth of microorganisms, characterized in that, in order to improve the accuracy of determination by more uniform growth of the test culture of microorganisms, vertical graded are installed in the container translucent tubes with the formation of a gap between their lower edges and the bottom of the tank to fill them with gel, while inside the tubes there are rods that are installed with the possibility of extraction, employees to form cavities in the gel for supplying air to the test culture of microorganisms. FIG. 2 2 / J /