SU1691391A1 - Method of fibroblast cultivation - Google Patents
Method of fibroblast cultivation Download PDFInfo
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- SU1691391A1 SU1691391A1 SU843787515A SU3787515A SU1691391A1 SU 1691391 A1 SU1691391 A1 SU 1691391A1 SU 843787515 A SU843787515 A SU 843787515A SU 3787515 A SU3787515 A SU 3787515A SU 1691391 A1 SU1691391 A1 SU 1691391A1
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- substrate
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- simplify
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Abstract
Изобретение относитс к биолоши v биотехнологии и может Ьыть использовано рп культивировани фибробластов. Целью изобретени вл етс упрощение способа культивировани фибробластов и повышение выхода жизнеспособных клеток. Это достигаетс использованием подложки из латекса натурального каучука, а также тем, что съем выросших клеток осуществл ют путем раст гивани подложки. 1 табл.The invention relates to bioloshes v biotechnology and can use rp fibroblast cultivation. The aim of the invention is to simplify the method of culturing fibroblasts and increasing the yield of viable cells. This is achieved by using a natural rubber latex substrate, as well as by removing grown cells by stretching the substrate. 1 tab.
Description
Изобретение относитс к биологии и биотехнологии и может быть использовано дл культивировани клеток, в частности, фибробластов .The invention relates to biology and biotechnology and can be used to cultivate cells, in particular fibroblasts.
Цель изобретени -упрощение способа культивировани фибробластов и повышение выхода жизнеспособных клеток.The purpose of the invention is to simplify the method of culturing fibroblasts and increasing the yield of viable cells.
Способ осуществл ют следующим образом .The method is carried out as follows.
Дл изготовлени подложки 2 мл жидкого латекса натурального каучука наливают на дно чашки Петри с площадью дна около 12 см2. После коагул ции латекса на воздухе и образовани пленки ее поверхность промывают бидистиллированной водой,стерилизуют 70%-ным раствором этанола в течение не менее 30 мин и ополаскивают питательной средой, например средой Игла . Затем на поверхность подложки нанос т суспензию клеток линии ВНК-21 в среде Игла без сыворотки в концентрации 10 тыс.To prepare the substrate, 2 ml of liquid natural rubber latex is poured onto the bottom of a petri dish with a bottom area of about 12 cm2. After coagulation of the latex in air and film formation, its surface is washed with bidistilled water, sterilized with 70% ethanol solution for at least 30 minutes, and rinsed with a nutrient medium, such as Eagle's medium. Then, a suspension of VNK-21 cells in serum needle at a concentration of 10 thousand was applied to the surface of the substrate.
клеток на 1 см подложки. После инкубации в известных услови х выросшие клетки снимают с подложки, слегка раст гива ее пинцетом и ополаскива в небольшом количестве среды Игла.cells per 1 cm substrate. After incubation under known conditions, grown cells are removed from the substrate, slightly stretching it with tweezers and rinsing in a small amount of Eagle's medium.
Результаты выращивани фибробластов в сравнении с известным приведены в таблице.The results of growing fibroblasts in comparison with the known are given in the table.
Таким образом, реализаци изобретени позвол ет повысить выход жизнеспособных клеток фибробластов на 30-35% и упростить способ культивировани ь-х.Thus, the implementation of the invention makes it possible to increase the yield of viable fibroblast cells by 30-35% and simplify the culture method.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU843787515A SU1691391A1 (en) | 1984-09-05 | 1984-09-05 | Method of fibroblast cultivation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU843787515A SU1691391A1 (en) | 1984-09-05 | 1984-09-05 | Method of fibroblast cultivation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU1691391A1 true SU1691391A1 (en) | 1991-11-15 |
Family
ID=21137312
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU843787515A SU1691391A1 (en) | 1984-09-05 | 1984-09-05 | Method of fibroblast cultivation |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU1691391A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007114726A1 (en) | 2006-04-06 | 2007-10-11 | Boris Karpovich Gavrilyuk | Monolayer wound coating and a method for the production thereof |
| WO2012115658A1 (en) * | 2011-02-25 | 2012-08-30 | Empire Technology Development Llc | Dynamically alterable cell support |
-
1984
- 1984-09-05 SU SU843787515A patent/SU1691391A1/en active
Non-Patent Citations (1)
| Title |
|---|
| Авторское свидетельство СССР Ns 1251528, кл. С 12 N 5/00, 1984. * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007114726A1 (en) | 2006-04-06 | 2007-10-11 | Boris Karpovich Gavrilyuk | Monolayer wound coating and a method for the production thereof |
| WO2012115658A1 (en) * | 2011-02-25 | 2012-08-30 | Empire Technology Development Llc | Dynamically alterable cell support |
| US9388379B2 (en) | 2011-02-25 | 2016-07-12 | Empire Technology Development Llc | Dynamically alterable cell support |
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