SU1687261A1 - Disinfectant agent - Google Patents

Abstract

The invention relates to sanitation and can be used for disinfecting the treatment of objects indoors. The purpose of the invention is to reduce toxicity and corrosivity. The disinfectant is an aqueous solution of polyhexene-methylenguanidip gluconate with the structural formula j-NH -C - NII- (f.H2 |; -li N11 tVli2Ov with the ratio of components in an aqueous solution, May% polyhexamethylene gluconate 0 5-1.5 water, the rest 5 gab

Description

The invention relates to sanitation and can be used for disinfecting objects in a closed room.

The purpose of the invention is the reduction of toxicity and corrosivity

The disinfectant is an aqueous solution of polichexane methylenguanidine gluconate with the structural formula

G-KN-S - Sh- (CH21k

Lk NH-C6H1207

when the ratio of components in water

solution, wt.%:

Polyhexamethyl-enguanidine 0.5-1.5 gluconate

WaterEverything

Polyhexamethylene guanidine gluconate

is obtained from polyhexamethylene guanidine hydrochloride. First, polyhexamethylene guanidine base (PGMG) is synthesized. To do this, dissolve in 200 ml (parts) of hot water (80–90 ° C) 100 g (parts) of pgmg hydrochloride. The resulting solution is added to 200 ml of sodium hydroxide solution prepared from 100 g (parts) of caustic soda and 100 ml (parts) of water Gather up a mass of alkaline solution (base PHMG), which is removed on the surface, and remove the alkali by washing the collected mass (base PHMG) with hot water. The base content of the PHMG in the resulting product is dried to a dry residue of 80-85 % Portions of 8.75 g (parts) of the obtained PHMG base are dissolved in 100 ml (parts) of a 10% aqueous solution of calcium gluconate, and then 3 ml (parts) of concentrated sulfuric acid are added to the resulting solution and the calcium sulfate is filtered off. The filtrate is dehydrated in a vacuum and complete

with

about

00

VJ GO

about

will wash the glyp onzt PHMG in the form of a solid product, White color without tzpakhe.

It is established that a disinfectant is used to protect the environment. aeruginosa Nr 103 m g of the rasmestella microorganisms of Staphylococcus chegez, strain 209 GIS n KMVPy. P. A Tarasovich Density vst: cn - 2 billion microbial bodies on 100 al1 surfaces. Infected objects are treated once with 0, ½ by 1.5% dilutions of dry zip j means and means. The bactericidal analysis washes from the surface of the microscopic analysis were carried out and processed and frost for 60 min. Pssme round 1 1111 -1 14- (mines with sterile M ljiiieiiuM tampsch ohm with the latter, anthracic nasal in MS with 2 ml of m of coarse broth (1LPP) and 0.1 ml per cups. Concentrate agar (MPA) as a control serves as a nsPraRotpniys drug test pbyeky. Weight from ITU gfG VO

, О 1 | -OOKpa HOv1 llf GORNOSCH.

ii ;; i og | delg-- 1, 1 I goshorg and -Dtc.cut zkg, cf- MC ii s itporo / Annvi n, hl leoitibK of a different type (sea guineas, Osipa spuctij, crawling (males and females) and age,

MYOCHENMA NUMBER OF ESTATE 14 ZHI

“N” groups of Я -0 s, GOST.

/ 1, FOREIGN SETTING ruipoid FOR CO. CM O r h

DRZZHCHYUG SMU ACTION HI GIOOG1; the preparations were applied in an open way to pre-arytepine skin plots dosing, per unit area m body mass of experimental animals. Test the conditions of the test of dusmatgali — an hour-long fixation of the experimental animals on the heel ppen.i of the exposure (for a month). The area is appli- cated in the study of the local dei ggie stateapl: dna white kris yugsm. marine image 25 cm, rabbits 56; m.

Local deG; steite was evaluated by means of an external palpatory h instrumental examination of the application sites. At the same time, the presence of erythemal reactions of peeling, necrosis of the skin turgor and its elasticity, the presence of infitration and edema were noted. Inaccuracy measured the local temprester and the thickness of the skin fold Allergenic de-types. studied the ne mongrel healthy youngsters in albinos in albinos using scientifically treated skin allergies and specific allergies to and / hrmschp for the month

five

)

0 j 0

 j

0 ri

O ,,

The cumulative ability was determined on white rats with daily for M-ch pts p ld-IOGPI1-. emulsified substances in doses 1/2, 1/5, 1/10, 1/20, LD50.

Comparative corrosion activity of drugs is judged by the time of corrosion corrosion after we use septile agents on g-ory ly and polished surface of metal (12X18H9T) plates (10x10 cm). 1 ml 0.5 ml rubbed onto the gas plates -1.5% of the aqueous solution of the disinfectant. To prevent the plates from drying out quickly, put the plates1 in a wet chamber (desiccator),

The use of the method is illustrated by examples.

Example 1. I Re metal plates (10x10), seeded with 2 billion of scattered mon ((organisms (E. coll, P. aerufjinosa, S. autct;), are subjected to a single-KpaTiioii PG process by wiping with 0.5% i One solution of polyac cyt wlolorya idine gluconate. To compare such plates, the three plates disseminated with the indicated microorganisms are treated once with rubbing with 0.5% water rastirsm hydrochloride pspigsamyt / nidine hydrochloride. . + PGK. And with the test surfaces make i washings with sterile gauze. Part 1 i pppp-). Desinating C) sdcT - oi Tbipaei total death of the literate pi.nsaifTL.HL- / Е coll. Fd. ) by; a and Gramphoid S. ateis of microorganisms.

Having eaten, the treatment with a known agent per plate revealed (M ± m) 50 ± 15 viable L coli cells and 160 ± 40 P. aeruginosa (but to the prototype).

Example 2. Three metal plates seeded with the indicated suspensions of test microorganisms are treated with 1% aqueous disinfectant solution. On plates treated with a polyhexamethyleneesanidine atom, -t.ii3Hccr: ocoui4 ix microorganisms i were not detected, and the same plates processed for comparison with polyhexamystyleneguanidine hydrochloride showed optical flots (34 ± 10) of P. aeruhydroorganisms.

EXAMPLE 3 Three metal plates, seeded with these suspended matter i eats microorganisms, are treated with a P% HI .m water solution of a disinfectant (means. On the plates treated with polyphenol polyamidene, viable test microorganisms are not loaded. However, single plates (28 ± 9) of P. aeruglnosa microorganisms were found on the same plates treated for comparison with psighexamethylene guanidine hydrochloride.

PRI me R 4. The shaved skin of the skin of 10 guinea pigs is wiped with a 0.5% aqueous solution of polyhexemethylene anidine gluconate daily for 20 days. Another group of animals (10 guinea pigs) were treated with polyhexamethylene guanidine hydrochloride. After 12 days, 8 animals treated with a solution of polyhexamethylene guanidine hydrochloride showed redness of the skin. In animals. treated with gluconate polyhexamethylene guanidine, for the entire 20-day period of observation of signs of skin-induced irritating effect was not detected.

EXAMPLE 5 A 10% solution (aqueous) of polyhexamethylene guanidine gluconate is applied daily to a shaved skin surface of 10 guinea pigs daily for 20 days. Another group of animals (10 guinea pigs treated the skin with polyhexamethylene guanidine hydrochloride. In the group of animals treated with a known agent, signs of skin-extending effect were found on day 10, and in the group of animals treated with the proposed remedy, they were not detected in the whole 20 Two days, 1st observation period.

EXAMPLE 6 A 1.5% aqueous solution of polyhexamethylene guanidine gluconate is applied to the shaved skin of the skin of 10 guinea pigs daily for 20 days. Another group of animals (10 guinea pigs) were treated with polyhexamethylene guanidine hydrochloride. In the group of animals treated with a known agent, signs of skin-remitting effect were found on the 5th day, and in the group of animals treated with the proposed agent were not detected for the entire 20-day observation period.

PRI me R 7. Three I mirror-polished surfaces of metal plates (12X18H9T) are applied once with 1 ml of 0.5% aqueous solution of polyhexamethylene guanidine gluconate. For comparison, 0.5 ml of a 0.5% aqueous solution of polyhexamethyleneguanidine hydrochloride is applied to the same plates. Plates to prevent rapid drying of the moisture are placed in a desiccator. On the plates treated with the proposed disinfectant, no traces of corrosiveness were found during the entire 72-hour observation period. On the surfaces of metal plates treated with a known agent, the appearance of a rust stain is noted 2-2.5 hours after application.

EXAMPLE 8. Three mirror-polished plate surfaces (12X18H9 0 are applied once with 1 ml of 1% aqueous solution of polyhexamethylenegluanide gluconate. For comparison, 1 ml of 1% aqueous solution is applied to the same plates. hydrochloride ioligexamethylene guanidine. Plates are placed in a desiccator. On the plates treated with the proposed disinfectant, no traces of corrosive activity are observed over the entire 72-hour observation period. On the surface of the metal plates treated with a known agent, e rust is noted 1.5-2.0 hours after application.

Example 9. Three three mirror-polished surfaces of metal plates П2Х18Н9Т) are applied once with 1 ml of a 1.5% aqueous solution of polyhexamethylene guanidine gluconate. For comparison, 1 ml of a 1.5% aqueous solution of polygamide Jung guanidine hydrochloride is applied to the same plates. Plates are placed in a desiccator. On the plates treated with the proposed disinfectant, no traces of corrosivity were observed over the 72-hour observation period. On the surfaces of metal plates treated with a known agent, the appearance of farther and rust is noted after 1-1 5 hours.

The data given in the examples are presented in tables 1-4. In tab. Figure 5 shows comparative data with the prototype on the minimum inhibitory concentration of antiseptic preparations.

While maintaining the bactericidal activity of the proposed disinfectant, toxicity was achieved by more than 4 times in any route of entry into the body, skin-irritant effect - by 2 times, and corrosivity - by more than 30 times. The coefficient of disinfection of the proposed remedy with an exposure of 1 h is 99.006%. The drug does not penetrate intact skin, does not accumulate in the body and does not have an allergenic effect due to its high molecular weight, it has a low volatility. Using it in the wiping mode in closed rooms in the presence of people does not require respiratory protection.

The disinfectant allows the current disinfection treatment in closed rooms in the presence of people.

Claims (1)

Invention Formula A disinfectant containing an aqueous solution of polyhexamethyleneguanidine salt, characterized in that, in order to reduce toxicity and corrosivity, polyhexamethyleneguanidine gluconate with the structural formula is used as polyhexamethyleneguanidine salt. jNH-C-NH- (ttl2) NH-C6Hi207 in the following ratio of components in aqueous solution, wt.%: Polyhexamethylene guanidine gluconate 0, 5-1.5 Water Remaining. ten Table 1 Comparative bactericidal activity of disinfection drugs Stressive data on the skin irritant effect of antiseptic agents Comparative toxicological evaluation of antiseptic drugs during intragastric and dermal route of intake. table 2 Table 3 Comparative data on the corrosive activity of antiseptics Minimally suppressive concentrations (IPC) of antiseptic drugs (mg / ml) Antiseptic Guest-culture microorganisms E. coll 026 P.aeruginosa No. 103 Polyhexamethyleneguanidine hydrochloride (prototype) Polyhexamethyleneguanidine gluconate Table 4 Table 5 103 S. a and re us number 209 0.07 ± 0.02 0.07 ± 0.02