SU1685253A3 - Method for producing a remedy with wound-healing activity - Google Patents

Abstract

The method of obtaining funds with wound healing i action. The invention relates to medicine, in particular to the medical industry, and concerns a method for producing an agent having wound healing activity. The purpose of the invention is the expansion of the spectrum of application due to the manifestation of antibody-stimulating activity. The method is carried out by diluting the cow's colostrum, taken 1-3 days after calving, separating a layer of cream. knocking oil out of it, melting it in a water bath, filtering, washing with water and drying. The method allows to obtain a drug that possesses not only wound-healing, but also anti-antibody-suppressing activity when administered orally. 1 tab.

Description

The invention relates to medicine, in particular to the medical industry, and concerns a method for producing an agent having a wound healing effect.

The purpose of the invention is the expansion of the spectrum of application due to the manifestation of antibody-stimulating activity.

The method is carried out as follows.

The tool is prepared from colostrum collected within 3 days after calving the cow. Take 10 liters of colostrum, add 2 liters of boiled water, mix thoroughly and pass through a separator, turning the screw that regulates the fat content of the cream by 1.5-2, more turnover than when separating ordinary milk. The cream collected for 1-2-3 days mix and churn butter. The whipped oil is placed in a water bath with a temperature of 80-90 ° C and melted for 1 hour. The melted fat is filtered through 2-3 layers of gauze, and then placed in a separating funnel, where it is washed 5-6 times with distilled water until it is washed. water will not become transparent. After the last wash, the separatory funnel with the resulting oil is immersed in a water bath at a water temperature of 70 ° C for 2-3 hours to remove the remaining water. The washed fat is drained into a dark glass dish. 500 g of the preparation is obtained, which is stored in a refrigerator at 4-5 ° C. The tool should be obtained from colostrum collected during the first 3 days of calving, as the content of active substances in it drops sharply.

The agent is a fatty fraction in which biologically and pharmacologically active substances are contained in a concentrated form. In appearance, the product is a homogeneous mass of orange with a faint odor of non-rancid oil. The agent is not an individual chemical compound and therefore does not have a chemical formula.

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By chemical composition, it is a mixture of glycerides (fats), which in turn consist of various fatty acids - saturated and unsaturated, liquid and solid, volatile and non-volatile, soluble in water and insoluble. According to the results of chemical analysis carried out in the laboratory of molecular bases of the Central Research Institute of Epidemiology of the USSR Ministry of Health, 91% of triglycerides, 10 3% of diglycerides, and traces of monoglycerides are contained in the product obtained. Free fatty acids are present in it in the amount of 1.2% cholesterol. 3.4%. fatty acid esters make up 1.6%, dry non-fatty residue 1.73%, 15 The product is practically free from protein, water contains not more than 1.8%. The solution of this agent contains 4-5 times more lipovitamins, 50-100 times more carotene and carotene, than in the fat 20 extracted from ordinary milk.

Wound healing activity was determined on outbred white rats after elimination of an alkaline burn. After treatment with this agent, the skin of 25 experimental animals was intact, while the skin of untreated animals was blackened by a chemical burn, and a black scab was formed at the burn site. The next day, there were also no 30 burn marks on the back of the treated rats. The skin remained white and supple. In animals treated by the time atom, the scab became even more pronounced. 8 a small percentage of cases, these animals even died.35

The wound healing effect was also studied on a model of uninfected extensive wounds in rats. The treatment was started from the second day after the operation. The remedy was applied to the wounds once a day until complete healing was 40. The remedy had a pronounced therapeutic activity and was comparable in activity to olive and sea buckthorn oil.

This tool also has a high regenerative activity in wound healing when measuring the tensile strength of the rumen,

The effect of the obtained agent on the antitoope formation process in rabbits is as follows. For immunization, a S. typhirnumum 1950 bacteria stamp was used in 1% neutral formalin.

First, the bacteria were sown on the nutritional nature of agar Hottinger, then incubated for 1 day in a thermostat at 37 ° C, after which the bacteria were reseed again on Hottinger agar and after 1 day washed with saline. For

Neutral formalin at a concentration of 0.5-1.2% was added to a test tube with bacteria to obtain a killed culture. One day after the treatment of bacteria with formalin in the indicated concentrations, a control seeding was done on Hottinger's agar. The growth of bacteria was observed only in the culture of bacteria with 0.5% formalin.

A suspension of bacteria was centrifuged twice in normal physiological solution for 20 minutes at 3000 xD. Washed from formalin sediment cells dissolved in 2 ml of saline. Then, gradually adding the saline solution to the tube and constantly measuring the suspension of the culture according to the standard, a concentration of 0.5 was obtained; one; 1.5; 2.0 billion microbial cells per ml.

In the experiments, groups of rabbits were used, 5 animals each, weighing 2-2.5 kg. Rabbits that make up the first group received a suspension of bacteria and a drug, rabbits of the second group received a comparison drug (a substance isolated by the same method, but from ordinary milk), rabbits of the third group received only a suspension of Salmonella bacteria.

Suspension of bacteria with increasing doses of 0.5; 1.0; 1.5; 2.0 billion microbial cells were injected in a volume of 1 ml intravenously 4 times with intervals between injections of 4-5 days. The resulting drug and comparator in the amount of 1 ml were orally administered daily throughout the entire course of immunization and blood collection. Rabbits from the experimental group received 23 g of the drug for the entire course, while rabbits from the control group each received 23 g of the comparison preparation

Blood for the study was taken from the ear vein on the 10th, 14th and 21st day after the last immunization. The obtained serum samples were titrated for the presence of antibodies in the reaction of passive hemagglutinitis (RPHA) with erythrocytes loaded with salmonella atigenome (typhimurium).

The results are presented in table.

As the data of this table showed, the obtained agent has a strong stimulating effect on the antibody formation process in rabbits. Moreover, an increased antibody titer in the blood of experimental rabbits is observed throughout the immunization. Comparison drug did not have any influence on the process of antibody formation. The resulting agent can be administered orally, with no toxic reactions or allergic manifestations in rabbits observed.

A similar process is observed in mice immunized with lipopolysaccharide isolated from S. anatum.

Claims (1)

The resulting product retains its activity well throughout the year. The invention The method of obtaining an agent having a wound-healing effect by treating the raw material with water, characterized in that, in order to expand the spectrum use due to the manifestation of antibody-stimulating activity, cows colostrum is used as raw material 1-3 days after calving, after treatment with water it is separated, the cream is separated, the oil is churned, then melted, filtered, washed with water at 70-80 ° C and dry.