SU1651199A1 - Method for quantitative determination of - and -carotene in biological materials - Google Patents

Abstract

The invention relates to analytical chemistry and can be used in agricultural production, the food industry, in health care in monitoring the content of micro-quantities and in-carotene in feed, food products and other biological materials. The purpose of the invention is to simplify the method and reduce the analysis time. The method includes saponification of the sample to be analyzed, extraction of carotenes with cyclohexane, input of the extract into the chromatographic column (sorbent – silousorb C18), subsequent separation of ft1 - and A kaR · amp; 2 tab. (L

Description

The invention relates to analytical chemistry and can be used to control the content of micro-quantities of tf- and p-carotene in feed, food and other biological materials.

The purpose of the invention is to simplify the method and reduce the analysis time.

The method includes the following operations: saponification of the sample to be analyzed, extraction of carotenes with cyclohexane, input of the extract into a chromatographic column with silensorb C18, subsequent separation (X- and p-carotene and separation Eh

treatment of interfering impurities in a stream of 1–2% isopropanol solution in cyclohexane.

Example 1. Determination of tf- and y-carotene in artificial solutions with and without the addition of vitamin A, E and carotenoids.

1-30 µl of the #- and / 3-carotene solution in cyclohexane with additives and without the addition of other carotenoids, vitamin A, E are introduced into the chromatographic column with Silorb C 18 liquid microcolumn chromatograph Milichrome and eluted with X- and -carotene 1 2 % isopropanol solution

with so

cyclohexane with detection in the UV spectrum at 274 nm. The calculation of the content of L- and / 5-carotene is carried out over the areas of their chromatographic peaks. The results of j determination of tf- and -carotene in artificial solutions are given in table 1.

Example 2. Determination of pd- and |} -carotnna in biological materials.

To 0.5-5.0 g of the analyzed sample, 5-20 ml of a 10% alcoholic KOH solution are added and hydrolysis of i is carried out. during 30 minutes at 60 ° C in an inert gas atmosphere. # And I-carotene are extracted with 1-5 ml of cyclohexane, 15 purified from impurities, 1 to 30 µl of the extract is introduced into the chromatographic column (sorbent siloresorb C 18) of a liquid microcolumn chromatograph Milichrome, and eluted with oi and i-20 carotene 1 - 2 % solution of isopropanol in cyclohexane with detection in the UV spectrum at 274 nm. The calculation of the content of the # - and jb -carotene is carried out over the areas of their chromatographic peaks. 25

The results of the determination (X- and p-carotene in biological materials are given in Table 2.

As follows from the table. 1 and 2 of the data, the proposed 3 method can be used to analyze any biological materials. The sensitivity of determination of # and / 3 carotene is 0.01 mg / l, which is sufficient for the analysis of biological materials, while the 1000-fold excess of other carotenoids, vitamins A and E do not interfere with the determination of tf and | 3-carotene.

Thus, the proposed method of quantifying the # .- and fi- carotene in biological materials has the following advantages as compared with the known method:

the method allows to simplify the determination of & and AND-carotene, since it does not require such complex operations as evaporation of the extract to dryness, dissolving the dry residue in a small amount of organic solvent and separating the interfering impurities on a preliminary chromatographic column;

Claims (1)

the method allows a 2-fold reduction in the analysis time by reducing the number of consecutive operations. Invention Formula The method of quantitative determination of 0Ј- and / 3-carotene in biological materials, including saponification of the analyzed sample, extraction of carotenes, introduction of a sample of the extract into a chromatographic column filled with a sorbent, and separation of the components of the sample in the eluent stream, characterized in that to simplify the process and reduce the time of analysis, cyclohexane is used as an extractant of carotenes, C 18 silorobor is used as a sorbent, and the separation is carried out in a stream of 1-2% isopropanol solution in cyclohexane. Carotenoids are isolated from plants on a chromatographic column and their total content is determined by absorption at 460 nm. Table 1 Table 2