SU1635042A1 - Method of sampling gas in vials - Google Patents
Method of sampling gas in vials Download PDFInfo
- Publication number
- SU1635042A1 SU1635042A1 SU884394417A SU4394417A SU1635042A1 SU 1635042 A1 SU1635042 A1 SU 1635042A1 SU 884394417 A SU884394417 A SU 884394417A SU 4394417 A SU4394417 A SU 4394417A SU 1635042 A1 SU1635042 A1 SU 1635042A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- vial
- sampling
- volume
- inert gas
- gas
- Prior art date
Links
Landscapes
- Sampling And Sample Adjustment (AREA)
Abstract
Изобретение относитс к гаэо- хроматографическому анализу содержани кислорода в герметичных флаконах с биопрепаратами. Цель изобретени - повышение точности анализа. Способ отбора пробы из флакона, включающий предварительный ввод инертного газа с последующим отбором пробы, заключаетс в вводе инертного газа во флакон, выравнивании в нем давлени до атмосферного, последующем вводе дозированного объема инертного газа и отборе пробы в количестве, равном объему дозы введенного инертного газа. 2 ил. с SSThe invention relates to a gas chromatographic analysis of the oxygen content in sealed vials with biological products. The purpose of the invention is to improve the accuracy of the analysis. The method of sampling from a vial, which includes the preliminary introduction of an inert gas followed by sampling, consists in introducing an inert gas into the vial, equalizing the pressure in it to atmospheric, then introducing a metered volume of inert gas and sampling in an amount equal to the volume of the inert gas injected. 2 Il. with SS
Description
Изобретение относитс к технике анализа состава газов, в частности к газохроматографическому анализу содержани кислорода в герметичных флаконах с биопрепаратами с помощью шприца.The invention relates to a technique for analyzing the composition of gases, in particular, to gas chromatographic analysis of the oxygen content in sealed biopreparations vials using a syringe.
Цель изобретени - повышение точности анализа.The purpose of the invention is to improve the accuracy of the analysis.
На фиг.1 и 2 изображена схема осуществлени предлагаемого способа.Figures 1 and 2 depict the implementation of the proposed method.
Газ (азот или гелий) из баллона 1 через редуктор 2 постзгпает в ресивер 4 и далее через шланг 6 и полую иглу 7 выходит в атмосферу.При этом давление в ресивере на 0,001 - 0,1 больше атмосферного. Перед отбором пробы ток газа между баллоном 2 и ресивером 4 перекрывают вентилем 3. Через несколько секунд давление в ресивере 4 становитс равным атмо сферному, что контролируют по дифференциальному манометру 5, который должен показывать нуль. Иглой 7 прокалывают пробку флакона и через 2-3 с давление в системе ресивер - флакон становитс примерно равным атмосферному . Чем больше объем ресивера, тем меньше разница давлений во флаконе и атмосфере, что видно из следующей формулы:Gas (nitrogen or helium) from the cylinder 1 through the reducer 2 is postzgpaet into the receiver 4 and further through the hose 6 and the hollow needle 7 is released into the atmosphere. At the same time, the pressure in the receiver is 0.001 - 0.1 more than atmospheric. Before sampling, the gas flow between the cylinder 2 and receiver 4 is blocked by valve 3. After a few seconds, the pressure in receiver 4 becomes equal to atmospheric pressure, which is controlled by a differential pressure gauge 5, which should indicate zero. The needle 7 pierces the cork of the vial and after 2-3 seconds the pressure in the receiver-vial system becomes approximately equal to the atmospheric pressure. The larger the volume of the receiver, the smaller the pressure difference in the vial and atmosphere, as can be seen from the following formula:
00
со Oiwith Oi
f где Рf where P
Р«т tP "t t
АBUT
ЧH
vp + vvp + v
- конечное давление во флаконе;- final pressure in the bottle;
р - атмосферное давление; д°р начальна разница давлений в атмосфере и флаконе;p is atmospheric pressure; d ° p is the initial pressure difference in the atmosphere and vial;
V - объем флакона;V is the volume of the bottle;
Vp - объем ресивера. Так, например, при объеме флакон 20 см 5 и объеме ресивера 2000 CMS ошибка определени концентрации кислорода в случае разрежени во флаконе уменьшаетс примерно в 100 раз, что вполне достаточно дл проведени анализа на уровне 0,1% содержани кислорода (нормы, рекомендованной ВОЗ дл сухих биологических препаратов).Vp is the receiver volume. For example, with a vial volume of 20 cm 5 and a 2000 CMS receiver volume, the error in determining the oxygen concentration in the case of a dilution in the vial is reduced by about 100 times, which is quite enough to perform an analysis at 0.1% oxygen content (the WHO recommended standard for dry biological products).
Иглу из флакона извлекают и в него ввод т шприцем газ-носитель в объеме, равном объему отбираемой пробы, и этим же шприцем отбираютThe needle from the vial is removed and a carrier gas is introduced into it in a volume equal to the volume of the sample taken, and the same syringe is taken
пробу дл последующего анализа его состава.sample for subsequent analysis of its composition.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU884394417A SU1635042A1 (en) | 1988-01-11 | 1988-01-11 | Method of sampling gas in vials |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU884394417A SU1635042A1 (en) | 1988-01-11 | 1988-01-11 | Method of sampling gas in vials |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU1635042A1 true SU1635042A1 (en) | 1991-03-15 |
Family
ID=21362134
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU884394417A SU1635042A1 (en) | 1988-01-11 | 1988-01-11 | Method of sampling gas in vials |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU1635042A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102052075A (en) * | 2009-10-30 | 2011-05-11 | 中国石油化工股份有限公司 | Sampling device, system for carrying out field sampling in oil field, method and application thereof |
-
1988
- 1988-01-11 SU SU884394417A patent/SU1635042A1/en active
Non-Patent Citations (1)
| Title |
|---|
| Лучинкин В.В. и др. Метод хранени и отбора без доступа воздуха жидких проб дл хроматографического анализа. - В кн.: Получение и анализ чистых веществ, вып.4.Йэд-во Горьковского универститета, 1979, с.106, 107. * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102052075A (en) * | 2009-10-30 | 2011-05-11 | 中国石油化工股份有限公司 | Sampling device, system for carrying out field sampling in oil field, method and application thereof |
| CN102052075B (en) * | 2009-10-30 | 2014-11-26 | 中国石油化工股份有限公司 | Sampling system for carrying out field sampling in oil field, method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Burris | [37] Nitrogen fixation—Assay methods and techniques | |
| Hintelmann et al. | Measurement of mercury methylation in sediments by using enriched stable mercury isotopes combined with methylmercury determination by gas chromatography–inductively coupled plasma mass spectrometry | |
| Capasso et al. | A simple method for the determination of dissolved gases in natural waters. An application to thermal waters from Vulcano Island. | |
| Kampbell et al. | Analysis of dissolved methane, ethane, and ethylene in ground water by a standard gas chromatographic technique | |
| Stainton | A syringe gas-stripping procedure for gas-chromatographic determination of dissolved inorganic and organic carbon in fresh water and carbonates in sediments | |
| Salata et al. | A rapid and precise method for measuring stable carbon isotope ratios of dissolved inorganic carbon | |
| Bergersen | Ammonia? An Early Stable Product of Nitrogen Fixation by Soybean Root Nodules FJ Bergersen | |
| Tesch et al. | Microdetermination of nitrates and nitrites in saliva, blood, water, and suspended particulates in air by gas chromatography | |
| IE43530L (en) | Collecting blood sample | |
| Inn et al. | Measurement of stratospheric sulfur constituents | |
| Williams et al. | Gas-liquid chromatographic determination of vinyl chloride in alcoholic beverages, vegetable oils, and vinegars | |
| SU1635042A1 (en) | Method of sampling gas in vials | |
| US5328823A (en) | Enzyme-based biosensors for detecting noble gases | |
| Maeda et al. | Simultaneous liquid chromatographic determination of water-soluble vitamins, caffeine, and preservative in oral liquid tonics | |
| Marcy et al. | A rapid method for the simultaneous determination of major organic acids and sugars in grape musts | |
| Rasmussen et al. | Determination of atmospheric halocarbons by a temperature-programmed gas chromatographic freezeout concentration method | |
| Tarli et al. | Gas-chromatographic determination of pantothenates and panthenol in pharmaceutical preparations by pantoyl lactone | |
| Heinemeyer et al. | Automated gas injector system for gas chromatography: Atmospheric nitrous oxide analysis | |
| Holt et al. | A laboratory technique for releasing and measuring denitrification products trapped in soil | |
| Rosen et al. | Analysis of vinyl chloride by mass fragmentography | |
| Berck | Potentiometric determination of phosphine | |
| Cook et al. | Measurement of oxygen, nitrogen, and carbon dioxide in beverage headspace | |
| Hysert et al. | Rapid gas chromatographic method for analysis of dimethyl sulfide in beer | |
| Anbar | Isotopic analysis of oxygen in water | |
| Sondack | GLC analysis of ergonovine maleate |