SU1630704A1 - Method selection of sorhum genotypes that can assimilate phosphorus compounds which are difficult of access - Google Patents

Abstract

The invention relates to agriculture, in particular to sorghum selection. Seeds of the original varieties are treated with a mutagen buffer solution. Cultivated plants are harvested from And and germinated in germinators containing phosphorus that is difficult to access. On the 5-7th day after the emergence of shoots, seedlings with red spotting are selected. Then they are grown in natural conditions up to the phase of full ripeness of seeds. In Mg, selected genotypes are tested for homozygosity by the ability to use hard-to-reach phosphorus. 1 tab.

Description

The invention relates to agriculture, in particular to sorghum selection.

Example. Take the original variety. sorghum seeds (Kubanska Runn and Efremovsk bela) are treated with a buffer solution of HMM-0.005% mutagen or 0.5% streptomycin for 18 hours. After they are washed in running water, dried and sown in soil. From the obtained plants, seed material M is obtained. The obtained seed material in the amount of 50,000 pieces, the Kubansk wound line and 30,000 pieces. Efremov Bela lines are sterilized with a mixture of 70% ethanol and 3% hydrogen peroxide in a 1: 1 ratio. Sterilized seeds are sown in a germinator, size (15 .20 cm) with a substrate of neutral perlite or

 clean calcined river sand. Substrate moistened with distilled

 water (pH 7.0). In the substrate do

lune holes (about 100 pieces per one germinator), one of the hard-to-reach organic or non-organic phosphorus compounds (bone on flour, tricalcium

phosphate, etc.).

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From above, pre-sterilized seeds are laid directly on the holes in the holes and covered with a substrate. Plant beds are placed in a dark thermostat and seeds are germinated at 28 ° C. After.; the occurrence of the first seedlings of germina with seedlings is exposed to daylight. On days 5–7 after the occurrence of sprouting on the leaves of plants capable of using phosphorus of difficult-to-reach compounds, a red spot appears, induced by excessive doses of absorbed phosphorus. This is confirmed by the data of chemical analysis of plants with a red spot and without it (see table) -, (L

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The total phosphorus content in the dry weight of seedlings of ordinary and phosphorus-effective (FEM) sorghum lines,%

thirty

As follows from the table, the total phosphorus content in plants with a red spot is 3-4 times higher than in plants without a red spot, which confirms the ability of genotypes with a red spot to seize difficult to reach Compound-1 no phosphorus. GV example of 50,000 pieces. Seed N sorghum. Kubanska early proposed method. Bom (in MZ) allocated 85 seedlings and 30,000 of them, the seed of sorghum Efremov bela 36 seedlings with clear symptoms of red spot. They are selected, the roots are washed with running water and planted in the soil to grow in natural conditions until full ripeness of the seeds. Seed progeny obtained from these plants are tested for homozygosity according to the test trait (in M5) for uptake of hard-to-reach phosphorus.

For this, from each ras-1 shade selected and brought to full ripeness (85 pieces, varieties of Korgansky sorghum

40

45

early and 36 pcs. The varieties of Efremov bela) take 300 seeds each and are sown in a germinative plant with the content of inaccessible phosphorus. For comparison, the same seeds are sown in germinator with the same substrate, but without hard-to-reach phosphorus compounds. Condition germination former. All seedlings in plants with a hard-to-reach phosphorus form 100% give a characteristic red leafiness of the leaves, while seedlings in plants that grow without hard-to-reach phosphorus remain green.

The plant genotypes selected by this method can be used as a starting material s for selection in obtaining varieties, lines capable of assimilating hard-to-reach phosphorus compounds.

Taking into account the peculiarities of the mineral nutrition of plants, such genotypes of plants eliminate the need for the introduction of phosphate fertilizers in full demand, which reduces the cost of the resulting environmentally friendly products, reduces environmental pollution by chemical compounds.

F o

 ula invention

A method of breeding sorghum genotypes capable of assimilating phosphorus of difficult-to-reach compounds, which consists in processing the seeds of the original forms with a mutagen, seed material is germinated on a substrate containing difficult-to-obtain phosphorus to obtain seedlings, seedlings with red spots are grown, and they are grown in natural conditions before obtaining the seeds, and in Ma, the isolated genotypes are tested for homozygosity in their ability to use phosphorus of hard-to-reach compounds.

Claims (1)

F o rm la invented chemistry n and I! sorghum, selection of genotypes to assimilate phosphorus of difficult compounds, consisting The method is capable of being available in that the seeds of the initial forms are treated with a mutagen, seed material M _{<5 is} obtained, germinated on a substrate containing inaccessible phosphorus until seedlings are obtained, seedlings with red spotting are isolated, they are grown under natural conditions to obtain M ^ seeds, and in M _{a, the} selected genotypes are checked for homozygosity for their ability to use phosphorus labor / inaccessible compounds.