SU1620476A1 - Apparatus for bacteriological analysis of air - Google Patents

Abstract

The invention relates to analytical microbiology, to devices for bacteriological analysis of air. The goal of eHoTeni is to increase the accuracy of analysis and simultaneous seeding of microorganisms on various media. The device comprises a housing 7 with a nozzle 2 for exhausting air, a cover 3 with slots 4 for supplying the analyzed air, placed inside the housing 1 table 5 with a plate 7, in recesses 8 of which cups 9 for the nutrient medium are installed. Table 5 is equipped with blades 12 for its rotation and a centrifugal regulator 13. The cups 9 are attached to the plate 7 by means of the clamp 14. The device provides simultaneous seeding of bacteria on different nutrient media while increasing the accuracy of the final aria-lease. 1 hp ff, 3 ill. eight

Description

This invention relates to analytical microbiology, in particular to devices for bacteriological analysis of air.

The purpose of the invention is to improve the accuracy of analysis and simultaneous seeding of microorganisms on different media.

FIG. 1 shows a device, a longitudinal section; in fig. 2 shows section A-A in FIG. one; in fig. 3 - knot in fig „1 (plate with cups).

The device for bacteriological analysis of air contains a cylindrical body 1 with a pipe 2 for sucking air, a round cover 3 with targets 4 for supplying test air and placed inside the case 1 under the cover 3 a circular horizontal table 5 mounted on axis 6

with the possibility of circular rotation, with a bacterium trapping means located on it, consisting of a circular plate 7 with circular depressions 8 in the upper part of the plate 7. The depressions 8 are evenly placed around the circumference of the plate 7 and the 9 hl nutrient / media cups are installed in them. The slots 4 in the lid 3 are radially evenly circumferentially above the cups 9, while the length 1

slots 4 is equal to the diameter d of the cups 9.

Table 5 with plate 7 and cups 9 is mounted with its axis 6 on a horizontal partition 10 and air holes 11 are made on its periphery, table 5 is equipped with blades 12 for its rotation and centrifugal rotation speed regulator 13. At the center of the plate 7 is placed

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retainer 14 for securing cups 9 in recesses 8.

A device for bacteriological analysis of air works as follows.

Before starting work, the cups 9 are filled with various nutrient media or with one medium and placed in the recesses 8 of the plate 7. Then the lid 3 is closed and the device is connected to the vacuum system (not shown) by means of the nozzle 2.

During operation of the device, the air under investigation is sucked through the slots 4 by the action of a vacuum in the housing 1, hits the surface of the plate 7 with 8 cups 9 located in the recesses and leaves 1 (impacts) the nutrient media contained in the air bacteria. Then the air flows around table 5 and passes through the holes 11 in the partition 10, interacting with the blades 12 on the way, driving table 5 into rotation, which ensures uniform distribution of bacterial aerosol particles over the entire surface of nutrient media, eliminating colony coalescence and accuracy of bacteriological analysis of air. To replace the substrates, open the lid 3, release the latch 14 and remove the cups 9 with the collected bacteria samples, cover with lids and subject to further analysis.

Sampling for several cups 9 with different elective media allows to expand the research capabilities of the device, namely, at the same time to investigate a certain complex of sanitary indicative and pathogenic microorganisms present in the air environment, while obtaining. t representation not only of their quantitative content in a unit volume of air, but also their percentage ratio. The accuracy of the analysis is achieved with the exception of the distortions observed during the sequential sampling of various electoral media and resulting from changes in the concentration of aerosol particles due to the destruction of biological aerodisperse systems, for example, when carrying out volumetric disinfection in the room, the passage of clouds formed by short-term emissions of harmful aerosols or simply due to changes in air flow direction

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in the presence of a constant source of air pollution.

The equality of the inner diameter of the cups 9 to the length of the impacting slit 4 for supplying air on the one hand eliminates wasteful consumption of the drawn in air, which occurs when the width of the annular sludge exceeds the diameter of the cup 9, and on the other hand prevents unproductive use of the nutrient surface in the cups 9 when their diameter is exceeded over the thickness of the annular sediment - the latter also complicates the determination of the fraction of the area of the medium in the plates 9 of the area of the annular sediment formed during the rotation of the table 5 under slit 4, since only a precise determination of the ratio of these areas allows the calculation of bacterial contamination of the air medium.

Thus, the device provides obtaining a given constant pattern of planting bacteria on nutrient media and a comparative analysis of air samples when grown on different nutrient media, which ultimately makes it possible to more accurately assess the bacteriological contamination of the surrounding air environment.

Claims (2)

1.A device for bacteriological analysis of air, containing a cylindrical body with a pipe for air suction, a round lid with slits for supplying test air and a round horizontal table, mounted on the axis with the possibility of circular rotation, placed on the axis under the lid for trapping bacteria, characterized in that, in order to increase the accuracy of the analysis and at the same time seeding microorganisms on different media, the means for trapping bacteria consists of a round plates with circular recesses in the upper part, placed evenly around the circumference and placed in the recesses by the cups for nutrient media, while the slits in the lid for the supply of air are located radially, evenly around the circumference above the cups. 2. A device according to claim 1, characterized in that the length of the slots - equal to the diameter of the cup. 1 / Y //// f //////// x L // //////////// l: AT / / XXXXXKX kXXX X XX 1 ten / J y / : $ S2ZZZZz $ B XXXXXKX kXXX X XX 1 ten A-fi FIG. 2 fie.j