SU1544381A1 - Method of determining functional state of flora and fauna organisms - Google Patents

Abstract

The invention relates to a laboratory technique and can be used to determine the functional state of plant and animal organisms, including for clinical studies. In order to increase accuracy, freezing of a biological fluid is carried out directly in a micropipette at one temperature, and volume changes are recorded by the level of the biological fluid using an optical device. 1 il.

Description

one

(21) 4428637 / 28-14

(22) 05/17/88

(46) 02.23.90 „Bull. № 7

(71) Smolensk State Medical Institute

(72) N.F.Farashchuk

(53) 615.478 (088.8)

(56) Laboratory work, 1974, No. 11, pp.677-680.

(54) METHOD FOR DETERMINING THE FUNCTIONAL CONDITION OF PLANT AND ANIMAL ORGANISMS

(57) The invention relates to laboratory technology and can be used to determine the functional state of plant and animal organisms, including for clinical studies. In order to improve the accuracy, the freezing of the biological fluid is carried out directly in a micropipette at one temperature, and the volume changes are recorded by the level of the biological fluid using an optical device. 1 il.

This invention relates to medicine and can be used to study the hydration of biocolloids and determine the functional state of plant and animal organisms, including humans.

The aim of the invention is to improve the accuracy of determining the functional state of plant and animal organisms.

The drawing shows the device (micropipette) used when implementing one of the steps of the method.

The method of determining the functional state is carried out as follows.

Micropipette 1 is filled with biological fluid to approximately the level of 0.08-0.09 ml (so that it does not go beyond the scale when the tissue expands) and is inserted into a special clamping device 2, which is a metal rod 3 with two horizontal areas 4 and 5. At the lower platform 5, a rubber gasket 6 is attached to which the lower end of micropipette 1 abuts, and in the upper platform there is an opening 7 for inserting the rod 8, which must fit tightly into the channel 9 of micropipette 1. Using an optical device ) region triple the volume of biological fluid with an accuracy of 0.00005 ml and bring the device with micropipet 1 into the freezing bath with a constant negative temperature for 15-20 minutes (until the tissue freezes. Then remove the device from the bath and through the opening 7 in the upper platform 4 enter In the micropipette 1, the rod 8 is pushed all the way into the frozen tissue at channel 9. At this time, the biological fluid thaws, its level decreases, and the rod 8 remains stationary, fixing the level of the frozen liquid. Using the specified optical device register the amount of frozen bi (L

SP

4 D

00 00

liquid according to the entered rod 8. Calculate the percentage of free water according to the formula

Yo

10,000

where Y is the initial volume of biological

liquid {jq

Y, is the volume of frozen biological fluid;

K is the compression coefficient of the biological fluid when cooled from room temperature to the crystallization temperature of water.

The total water is determined by drying the fabric at 100-105 ° C, followed by weighing, which is related by the difference in po between total and free.

Example. Determination of water fractions in the blood of a healthy person. In the usual way, blood is drawn from a finger, 25 into a micropipette 1 approximately up to a division of 0.09 ml and placed in a clamping device 2. The exact volume of blood is determined using an optical device. It is 0.09225 ml. 30 Place the clamping device 2 with a micropipette 1 in a freezing bath with cooling liquid with a temperature of -10 ° C, after 20 minutes remove the metal rod 8 into the upper opening of the micropipette until it stops. Filter paper removes from the micropipette 1 the remnants of the coolant (alcohol) and again using an optical device on the bottom to the end of the rod 8 register the volume of tissue. He became equal 0,09665 ml. The micropipette 1 is removed from the clamping device 2, the thawed blood is released onto a pre-weighed 45 glass and weighed on an analytical balance. The mass of blood is 0.08121 g. Dry the blood at 10 ° C to constant weight and re-weigh. The dry blood mass is Q 0.016655 g. The coefficient of blood compression ranging from room temperature to the crystallization temperature of free water in experiments is 0.0023, which approximately corresponds to a coefficient of water compression in this temperature range. The percentage of free water in the test blood is

0 09225 Og0023 + (0A09665-0A09225) 0.09225 8, zTb-

“10,000 60.01%.

The percentage of total water is

. I 0A ° 1§§55 0.08121

79.49%

The content of bound water is equal to the difference between total and free: 79.49- -60.01 19.48%.

The advantages of the method are as follows. The amount of biological fluid required for research is significantly reduced, which makes it possible to use the method in clinical laboratory studies, including for mass screening of both sick and healthy people. For its implementation, a drop of blood from the patient’s finger is sufficient.

Labor consumption is reduced, the number of operations is reduced. It is not necessary to work with a dilatometer and a working fluid, to freeze at three different temperatures, to constantly monitor the level of the liquid in the micropipette and repeatedly register it, to put in parallel control experience. Essentially, the work goes on with only one, the most essential part of the dilatometer - the micropipette. This allows you to work with several samples at once. For example, you can simultaneously take blood from 5-10 patients, mark its volume and freeze all samples at the same time in one bath. With the same skills in the work method several times reduces the time spent. During the working day, the proposed method can examine 15-20 blood samples, and with a known method, only 2-3 samples.

I

The accuracy of the method increases.

the use of an optical device that allows the division of the micropipette into 20 parts. In addition, the exclusion from the method of precast dilatometer and working fluid eliminates its rapid evaporation both through the upper hole of the micropipette and through the surface.

5154

connecting parts, which affects accuracy.

Claims (1)

Invention Formula The method of determining the functional state of plant and animal organisms, including determining the amount of free water in a biological fluid by freezing it and recording its volume and determining the bound water by drying and weighing the biological fluid, taking into account the amount of total water u1 due to the fact that, in order to increase the accuracy of determination, freezing of free water in a biological fluid is carried out in a micropipette, and after the biological fluid freezes, a rod is inserted into the micropipette channel until it stops in the frozen liquid with a minimum clearance relative to the walls Water is made from the difference in biological fluid levels before and after freezing with an optical device.