SU1538513A1 - METHOD FOR ISOLATION OF HUMAN SOMATOTROPIN - Google Patents
METHOD FOR ISOLATION OF HUMAN SOMATOTROPINInfo
- Publication number
- SU1538513A1 SU1538513A1 SU3669173/14A SU3669173A SU1538513A1 SU 1538513 A1 SU1538513 A1 SU 1538513A1 SU 3669173/14 A SU3669173/14 A SU 3669173/14A SU 3669173 A SU3669173 A SU 3669173A SU 1538513 A1 SU1538513 A1 SU 1538513A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- carried out
- isolation
- somatotropin
- human
- chromatography
- Prior art date
Links
Landscapes
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Способ выделения соматотропина человека, включающий дезинтеграцию клеток биомассы бактерий Escherichia coli, несущих рекомбинантную плазмидную ДНК, обработку полиэтиленимином, фракционирование сульфатом аммония и гельхроматографию, отличающийся тем, что, с целью увеличения степени очистки препарата, дополнительно проводят хроматографию на анионообменной целлюлозе, далее раствор концентрируют и гель-хроматографию проводят дважды, а полученный раствор диализуют и лиофилизируют.A method for isolating somatotropin human, comprising disintegration of a biomass of cells of bacteria Escherichia coli, harboring the recombinant plasmid DNA polyethyleneimine treatment, ammonium sulfate fractionation and size exclusion, characterized in that, in order to increase the degree of drug treatment in addition is carried out by chromatography on an anion exchange cellulose, hereinafter, the solution is concentrated and gel chromatography is carried out twice, and the resulting solution is dialyzed and lyophilized.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU3669173/14A SU1538513A1 (en) | 1983-12-05 | 1983-12-05 | METHOD FOR ISOLATION OF HUMAN SOMATOTROPIN |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU3669173/14A SU1538513A1 (en) | 1983-12-05 | 1983-12-05 | METHOD FOR ISOLATION OF HUMAN SOMATOTROPIN |
Publications (1)
Publication Number | Publication Date |
---|---|
SU1538513A1 true SU1538513A1 (en) | 2000-04-20 |
Family
ID=60527290
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SU3669173/14A SU1538513A1 (en) | 1983-12-05 | 1983-12-05 | METHOD FOR ISOLATION OF HUMAN SOMATOTROPIN |
Country Status (1)
Country | Link |
---|---|
SU (1) | SU1538513A1 (en) |
-
1983
- 1983-12-05 SU SU3669173/14A patent/SU1538513A1/en active
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