SU1538513A1 - METHOD FOR ISOLATION OF HUMAN SOMATOTROPIN - Google Patents

METHOD FOR ISOLATION OF HUMAN SOMATOTROPIN

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Publication number
SU1538513A1
SU1538513A1 SU3669173/14A SU3669173A SU1538513A1 SU 1538513 A1 SU1538513 A1 SU 1538513A1 SU 3669173/14 A SU3669173/14 A SU 3669173/14A SU 3669173 A SU3669173 A SU 3669173A SU 1538513 A1 SU1538513 A1 SU 1538513A1
Authority
SU
USSR - Soviet Union
Prior art keywords
carried out
isolation
somatotropin
human
chromatography
Prior art date
Application number
SU3669173/14A
Other languages
Russian (ru)
Inventor
В.А. Ежов
П.М. Рубцов
А.А. Баев
А.Г. Козловский
Н.М. Кузьмина
П.С. Свердлова
К.Г. Скрябин
Original Assignee
Институт биохимии и физиологии микроорганизмов
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Институт биохимии и физиологии микроорганизмов filed Critical Институт биохимии и физиологии микроорганизмов
Priority to SU3669173/14A priority Critical patent/SU1538513A1/en
Application granted granted Critical
Publication of SU1538513A1 publication Critical patent/SU1538513A1/en

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  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Способ выделения соматотропина человека, включающий дезинтеграцию клеток биомассы бактерий Escherichia coli, несущих рекомбинантную плазмидную ДНК, обработку полиэтиленимином, фракционирование сульфатом аммония и гельхроматографию, отличающийся тем, что, с целью увеличения степени очистки препарата, дополнительно проводят хроматографию на анионообменной целлюлозе, далее раствор концентрируют и гель-хроматографию проводят дважды, а полученный раствор диализуют и лиофилизируют.A method for isolating somatotropin human, comprising disintegration of a biomass of cells of bacteria Escherichia coli, harboring the recombinant plasmid DNA polyethyleneimine treatment, ammonium sulfate fractionation and size exclusion, characterized in that, in order to increase the degree of drug treatment in addition is carried out by chromatography on an anion exchange cellulose, hereinafter, the solution is concentrated and gel chromatography is carried out twice, and the resulting solution is dialyzed and lyophilized.

SU3669173/14A 1983-12-05 1983-12-05 METHOD FOR ISOLATION OF HUMAN SOMATOTROPIN SU1538513A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SU3669173/14A SU1538513A1 (en) 1983-12-05 1983-12-05 METHOD FOR ISOLATION OF HUMAN SOMATOTROPIN

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SU3669173/14A SU1538513A1 (en) 1983-12-05 1983-12-05 METHOD FOR ISOLATION OF HUMAN SOMATOTROPIN

Publications (1)

Publication Number Publication Date
SU1538513A1 true SU1538513A1 (en) 2000-04-20

Family

ID=60527290

Family Applications (1)

Application Number Title Priority Date Filing Date
SU3669173/14A SU1538513A1 (en) 1983-12-05 1983-12-05 METHOD FOR ISOLATION OF HUMAN SOMATOTROPIN

Country Status (1)

Country Link
SU (1) SU1538513A1 (en)

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