SU1528435A1 - Method of revealing calcium ions in neuron structures - Google Patents

Method of revealing calcium ions in neuron structures Download PDF

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Publication number
SU1528435A1
SU1528435A1 SU874255912A SU4255912A SU1528435A1 SU 1528435 A1 SU1528435 A1 SU 1528435A1 SU 874255912 A SU874255912 A SU 874255912A SU 4255912 A SU4255912 A SU 4255912A SU 1528435 A1 SU1528435 A1 SU 1528435A1
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SU
USSR - Soviet Union
Prior art keywords
solution
acetone
calcium ions
potassium
mononeurons
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SU874255912A
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Russian (ru)
Inventor
Владимир Владимирович Казанский
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Институт Физиологии Им.И.П.Павлова
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Abstract

Изобретение относитс  к медицине и может быть использовано в гистологии. Целью изобретени   вл етс  улучшение способа вы влени  ионов кальци  в структурах нейрона и сокращение сроков фиксации исследуемой ткани. Способ заключаетс  в добавлении в фиксирующий раствор четырехокиси осми  2%-ного раствора пироантимоната кали  в равных пропорци х (1:1).This invention relates to medicine and may be used in histology. The aim of the invention is to improve the method of detecting calcium ions in the structures of the neuron and to reduce the time of fixation of the examined tissue. The method consists in adding a 2% solution of potassium pyroantimonate in equal proportions (1: 1) to the fixing solution of osmium tetroxide.

Description

(21)4255912/28-14(21) 4255912 / 28-14

(22)03.04.87(22) 04/03/87

(46) 15. 12.89. Бюл. N 46 (1) Ингтитут физиологии им. И.П.Павлова(46) 15. 12.89. Bul N 46 (1) Ingtitu physiology them. I.P.Pavlova

(72) В.В.Казанский (53) 615.475 (088.8) (56) Ки щенко Л.И., Павленко В.К. Ультрас:тр уктурна  локализаци  ионов к альцн  п механорецепторных нейронах рецептора раст жени  речного рака.(72) V.V. Kazansky (53) 615.475 (088.8) (56) Ki Schenko L.I., Pavlenko V.K. Ultras: transduction of ions to alcn p of mechanoreceptor neurons of crayfish stretch receptor.

Питологи , 1981, 23.Psychologists, 1981, 23.

с.89-90.pp.89-90.

(54)СПОСОБ ВЫЯВЛЕНИЯ ИОНОВ КАЛЬЦИЯ В СТРУКТУРАХ НЕЙРОНА(54) METHOD FOR DETECTING CALCIUM IONS IN NEURON STRUCTURES

(57) Изобретение откоситс  К мелицине и может быть использовано в гистологии . Цепь изобретени  - улучшение способа вы влени  ионов кальпи  в структурах нейрона и сокращение сроков фиксаи и исследуемой ткани. Способ заключаетс  в добавлении в фиксирующий раствор четырехокиси осми  2 -ного раствора пироантимоната кали  в равных пропорци х (1:1).(57) The invention skims Melicin and can be used in histology. The circuit of the invention is an improvement in the method of detecting calpi ions in the structures of a neuron and shortening the time of fixation and tissue under study. The method consists in adding a 2-potassium pyroantimonate solution in equal proportions (1: 1) to the fixing solution of osmium tetroxide.

Изобретение относитс  к медицине иThe invention relates to medicine and

может быть И(,ггс:льзовано в гистологии.may be And (, ghz: lzlovo in histology.

Цель И ,с1бретени  - улучшение спо- с:обл и сокращение сроков фиксации.Goal And, from 1bt, it is to improve the way of: region and reducing the time of fixation.

Способ осуществл ют путем добавлени  в фикси;1ую1ций раствор четьфехоки- си осми  , 2%-ного пироантимоната кали  в iiaBHbix пропорци х (1:1).The method is carried out by adding to the fix; 1st solution, a solution of four osteocaphochic acid, 2% potassium pyroantimonate in iiaBHbix proportions (1: 1).

Claims (1)

П р и м е р. Препарат - мононейроны спинпого мозга л гушки получают путем обработки спинного мозга препарированного животного тампоном, смоченным в фиксир:ло1гдем растворе - четырех- окись осми  и 2%-нь й раствор пироантимоната кали  в равных пропорци х, в течение 5 мин. Последующую обработку провод т по схеме электронно-микроскопического вы влени  кальц11 . На электронограммах гранулы пироантимоната кали  равномерно распределены по всей цитоплазме мононейронов. Формула изобретени PRI me R. The preparation — mononeurons of the brain's spinal cord — is obtained by treating the spinal cord of an animal prepared with a swab moistened in fix: with a solution — osmium tetroxide and 2% potassium pyroantimonate solution in equal proportions for 5 minutes. Subsequent processing is carried out according to the scheme of electron microscopic detection of calcium 11. Potassium pyroantimonate granules on electron diffraction patterns are evenly distributed throughout the cytoplasm of mononeurons. Invention Formula Способ вы влени  ионов кальци  в структурах нейрона путем фиксации срезов ткани в водном растворе четырех окиси осми , обработки последовательно спиртом, смесью спирта с ацетоном (151), далее ацетоном, пропитки смесью ацетона с аралдитом, заливки в капсулы чистым аралдитом с последующим термостатированием и исследованием на электронном микроскопе, отличающийс  тем, что, с целью улучшени  способа и сокращени  сроков фиксации исследуемой ткани , в водный раствор четырехокиси осми  добавл ют 2%-ный раствор пироан- тимоиата кали  в равных пропорци х (1:1).The method of detecting calcium ions in neuron structures by fixing tissue sections in an aqueous solution of four osmium oxides, processing successively with alcohol, a mixture of alcohol with acetone (151), then acetone, impregnating with a mixture of acetone with araldite, pouring into capsules with pure araldite followed by thermostating and testing on an electron microscope, characterized in that, in order to improve the method and reduce the time of fixation of the tissue being examined, a 2% solution of potassium pyroanthymioate in equal amounts is added to an aqueous solution of osmium tetroxide oportsi x (1: 1). (Л(L
SU874255912A 1987-04-03 1987-04-03 Method of revealing calcium ions in neuron structures SU1528435A1 (en)

Priority Applications (1)

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SU874255912A SU1528435A1 (en) 1987-04-03 1987-04-03 Method of revealing calcium ions in neuron structures

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SU874255912A SU1528435A1 (en) 1987-04-03 1987-04-03 Method of revealing calcium ions in neuron structures

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SU1528435A1 true SU1528435A1 (en) 1989-12-15

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