SU1515862A1 - Chamber for preparing frozen biological objects for examinations - Google Patents

Abstract

The use of bentonization refers to the analysis of biological research and can be used, for example, in the processing of tissue for microscopic examination. The aim of the invention is to improve the quality of the prepared object. The frozen bioobjects are placed in perforated containers 6 and placed in containers 3 located in housing 1 around central channel 2. When rotating the booster 7 of the medium flow, the replacing ice medium circulates between the central channel 2, where it is cooled by the conical surface of the refrigerant 10, and capacitance. 3, where laminar flow and replacement fluid are created. In this case, the identity of the biomass processing is ensured, rtpoqecc replacement is accelerated. 2 Il. t ate SL 00 Od 1C

Description

31515

The invention relates to biological research and can be used, for example, in tissue processing for microscopic examination.

The aim of the invention is to improve the quality of an object prepared for research.

In figure 1, the image on the cryochamber

for the preparation of frozen bioobjects for research, longitudinal section; figure 2 is the same, top view.

The cryochamber includes a cylindrical fluoroplastic housing 1 with a central channel 2, four tanks 3 arranged around it, connected by lower inflow channels A and upper inflow channels 5 with a central channel 2. In each

Cylindrical perforated containers 6 with frozen b and object are fastened from the containers 3 in the middle one by one. The bottom and roof of the containers 6 are made of metal mesh with a hole size of 0.25 mm. In the central channel 2 (in its middle zone) there is installed a medium flow booster 7 replacing ice in a bioobject installed on piTOKe 8 exiting through channel 9. In the lower part of the central channel 2, the refrigerant line 10 with a conical surface 11 is strengthened. sealing plugs 12. In one of the containers 3 through the channel 13 entered the sensor 14. In the center of the housing 1 passes the reference cone 15.

The camera is operated as follows.

The frozen bioobjects are placed in cylindrical perforated containers 6, which are each installed in containers 3 filled with cooled replacement medium. The containers 3 are sealed by the tube and 12. When rotating the booster 7 of the medium flow in the central channel 2, the ice-sweeping medium circulates between the central channel 2 where it is cooled by a conical surface 11

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Claims (1)

propodp 10, and capacitors 3 on the lower and upper avenue of the receiving channels, 5. The conic surface 1 1 of the cold storage conduit 10 serves to reflect the flow of substituting medium into the lower flow channels 4 located at an angle close to direct to it. Manufacturing of the fluoroplastic body 1 contributes to stabilizing the temperature of the substituting medium. Due to the presence of the central channel 2 with the booster 7 placed in its middle zone, the fluid flow in the containers 3 creates laminar flows of the replacement liquid, which circulates through the lower 4 and upper 5 transfer channels 1 between the central channel 2 and the containers 3. Size perforated containers 6 with a frozen bioobject, one at a time in a container 3, provides for the creation of identical washing conditions for the frozen bioobjects in containers. The identity of the substitution conditions, the laminar flow of the substitution fluid determine the identity of the biomaterial processing (a, speeds up the substitution process, which increases the morphological accuracy of the information being removed, reduces the likelihood of artifacts. Formula of the invention for the preparation of frozen biological objects for studies containing a cylindrical body with containers the length of the perforated containers with the bioobject, the stimulator of the flow of the medium that emulates the ice in the bioobject, is cold, due to the fact that - The aim is to improve the quality of the prepared object, a central channel is made in the corgus, containers for containers are located around it and communicated with it in the upper and lower parts by the overflow channels, while the rear of the medium that replaces the ice is installed in the central channel, in its middle zone, and the cold pipeline has a section with a conical surface, reinforced at the bottom of the central channel. one 12 FIG. 2