SU1514764A1 - Nutrient medium for identifying mildew fungi affecting lubricant-coolants - Google Patents

Description

The invention relates to microbi2

and concerns nutrient media for identifying, quantifying, and studying the cultural characteristics of mold fungi affecting coolants. The aim of the invention is to improve the quality of the nutrient medium by accelerating the growth of mold fungi while simultaneously suppressing the growth of bacteria and maintaining the pH at an optimal level. The technical solution is that in a known nutrient medium containing sucrose, mineral salts, agar and water, tannin is additionally introduced in an amount of 0.3-0.52 !. The proposed environment provides accelerated (in 24-48 hours) detection and quantitative accounting of fungi that affect coolant. 7 tab.

The invention relates to Microbiology and relates to nutrient media for the detection, quantification and study of the cultural characteristics of mold fungi, affecting coolant (coolant).

The purpose of the invention is to improve the quality of the nutrient medium by accelerating the growth of mold fungi while simultaneously suppressing the growth of bacteria and maintaining the pH at an optimum level.

In a known medium containing sucrose, potassium phosphate acid monosubstituted, potassium phosphoric acid disubstituted, magnesium sulfuric acid,

sodium nitric acid, potassium chloride, sulfuric acid iron, agar-agar and water, additionally injected tannin at a concentration of 0.3-0.52.

The nutrient medium is prepared as follows. The required number of medium components is poured with distilled water, put on for 40 minutes to swell microbial agar, bring to a boil and filter through a cotton-gauze filter. You can prepare and liquid medium with tannin, excluding agar. Medium agar - dense, without agar - liquid, does not require autoclaving.

1514764 A1

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For comparative tests prepared 5 option ¹ :! nutrient medium, the composition of which is presented in table. one; physical and chemical properties of media - in table. 2 and 3.

To assess the effectiveness of the proposed medium, its comparative tests are carried out with the well-known ChapekDox medium taken in microbiology by the Dani method. The essence of the methods lies in the sowing of nutrient media by microorganisms and their subsequent exposure to conditions favorable for the growth of these microorganisms. Nutrient media 15 are inoculated with a mixture of mushrooms, pure cultures of AvregdSizggee, Azreg £ 111 fungi, a mixture of bacteria isolated from the coolant of the plant immediately before testing. 20

Analyze also 27 samples coolant Carbamol 111, Avtokat, Ukrinol 1M, naturally infected with microorganisms in the circulation systems at the plant (working coolant). The growth control of 25 microorganisms is carried out visually by the amount of biomass, as well as by the number of fungi, bacteria, determined by the pan method. The test results are shown in Table. 4-6. zo

As can be seen from the table. 4 and 5, sample 1 of the proposed medium should be excluded from further tests due to insufficiently active growth of fungi and insufficient inhibition of bacterial growth, sample 5 of agarized medium due to semi-liquid consistency, and liquid due to poor detection of deep growth of fungi in too dark environment. 40

Samples of media 2-4 provide for the germination of fungi much larger than on the environment of čapek-Doksa, and their subsequent more active growth. In addition, the introduction of tannin leads to 45 maintaining the pH of the medium at 4.5–4.6, which is optimal for the growth of most fungi and therefore does not require its adjustment during the growing process (Table 6). 50

Nutrient medium with tannin does not require autoclaving, since tannin prevents the growth of bacteria.

With the growth of colonies of fungi around them formed a zone of enlightenment in the form of rings with a thickness of 1.8-2.2 mm. But the obtained data tannin is used by mold fungi as an additional carbon source, as a result of which the growth rate of fungi on the medium with tannin increases dramatically.

PRI me R. The prepared medium is poured into 9 MP in test tubes. Then they are made of 1 ml of the test medium or coolant and incubated for 1824 hours under conditions that are favorable; ·: for the growth of fungi. Assessment of the growth of fungi is carried out by the amount of biomass visually: zero points - no growth; 1 point - single microcolonies (up to 10); 2 points - single microcolonies (more than 10 pieces) or biomass in the form of a discontinuous ring; 4 points biomass in the form of a continuous layer of different thickness. The results are shown in Table. 7

As can be seen from the table. 7, use the proposed among! with tannin allows you to quickly, much earlier than the visible growth of fungi usually begins, to detect them and determine the number in points.

Thus, the proposed environment provides an accelerated (after 24–48 h) identification, quantitative accounting and rapid study of the cultural features of the fungi affecting the coolant, which allows us to take measures to prevent their growth and, consequently, improve the physico-chemical, technological , sanitary and hygienic and operational indicators of coolant. In addition to a quick study of the mushroom contamination by coolant, the proposed medium can be used for expresscontrol of other materials: food, oil products, water and aqueous solutions, and air for pollution by fungi.

Claims (1)

Formula isoooret of Nutrient medium for the detection of mold fungi affecting coolants, containing sucrose, potassium phosphate-acidic monosubstituted, potassium phosphate disubstituted, magnesium sulfate, sodium nitrate, potassium chloride, iron sulfate, agar-agar, and distillated and discharged, and agar-agar and distilled acid, sodium nitrate, potassium chloride, iron-sulfuric acid, agar-agar, and distilling, and sodium nitrate, potassium chloride, iron-sulfate, agar-agar, and sulfate-acid; and h and ιοί !! and I am with the fact that, in order to improve its quality by accelerating the growth of mold fungi while simultaneously suppressing the growth of bacteria and maintaining the pH at an optimum level, it is up to 1514764 The supplement contains tannin in the following ratio of components, g / l: Potassium phosphoric acid monosubstituted $ 0.6-0.8 Potassium phosphoric acid disubstituted 0,2-0,4 Sulfuric Magnesium 0.4-0.6 Sodium nitrate 1.5-2.5 Potassium chloride Iron sulfuric acid Sucrose Tannin Agar agar Distilled water 0.4-0.6 0,008-0,013 28-32 3-5 18-22 Up to 1 l Table 1 Name of components, g / l Environment options one T ² G 3 | five Potassium phosphoric acid ly monosubstituted 0.5 0.6 0.7 0.8 0.9 Potassium phosphoric acid little disubstituted 0.1 0.2 oh w 0.4 0.5 Magnesium sour 0.3 0.4 0.5 0.6 0.7 Sodium Nitrate 1.0 1.5 2.0 2.5 2.8 Potassium chloride oh w 0.4 0.5 0.6 0.7 Iron sour 0,005 0,008 0.01 0.013 0.02 Sucrose 27.0 28.0 29.0 30.0 31.0 Agar 16,0 18.0 20.0 22.0 24.0 Tannin 1.0 3.0 4.0 5.0 7.0 Distilled water Before 1 l table 2 Characteristics of agar nutrient media Indicator Known Wednesday ChapekDox Options for the proposed environment one 2 3 four five Colour Gray Light brown Gray Consistency Dense Dense Dense Half tion liquid ph 6.25 5.11 4.80 4.71 4.64 4.54 T a b l and cc 3 Characteristics of liquid nutrient media Indicator Known Wednesday ChapekDox Options for the proposed environment Transparency Transparent Transparent Transparent Transparent Share 7 1514,764 δ Pre. '! *? Table. .3 Indicator Colour pH Known Wednesday ChapekDox Options for the proposed environment I Nestsvet- Light brown Korich- Dark other brown 6.20 5.29 4.81 4.60 4.50 4.38 Б and blitz 4 Characteristics of the growth of microorganisms in different variants of dense nutrient media. Dense Nutrient Options mediums Microbial growth after 24 h Clean cultures mushroom Mushroom Mixture With me bacteria PL medium without d) ztoklavyro potassium and zarese;, icroorganisms one + 4- + + + 2 +++ + + + - - 3 ++ + + + + - - four + -Y + + + - - five Wednesday excluded from because of liquid consistency Known Started through Wednesday 7 days + Capek-Doksa + + (Bacterium Note: +++ - the growth of fungi in zhitskoy environment is abundant deep and superficial with good sporulation. Colonies on a dense medium are large, fluffy with good curing. The color is bright. There are clear zones of enlightenment around the colonies. The growth of bacteria on a dense medium is large, the colonies are large, + + - growth of fungi or bacteria is moderate. + - the growth of fungi (bacteria) is weak. Koloim mushrooms flat. Dispute wearing ep nnchnoe or absent. - - microbial growth is absent. Have 1 514764 I) T a b l and c a 4 The growth characteristics of mihros-rga "ismi on different variants are liquid: nutrient media Variants of liquid nutrient media Microbial growth after 24 h ------------------- See bacteria si (inoculation up to; 0 cells / ml) On Wednesday? autocooler and microorganism seeding Clean cultures mushroom Mixes! mushroom I ί one one one 4-4 44 4 ¹ 0 - 2 44- 4 44 - - 3 44 4 4 4-4 - - four 4 4 4 44 4 - - five 444 4 4 4 - - Known Deep Growth ” browsing /: +! · ΠΪΟΧΟ <0 ten four Capek-Doksa Started growth of eez (Bacteria) 7 С 7Т Note: see tab. Table 6 The change in pI is proposed.! ": The environments and environments of ČapekDox a week after cultivation species ~ ’.'IboW Mushroom species Wednesday of čapek - Dt sa, initial value-c'chs pH 4.7 G "~ The proposed Erida 3, the initial pH value of 4.6 AzregDPiz ηΐβετ 4.0 4.6 ΑδρεΓβίΙΙ .пн ogtgay 4.05 4.7 Note; the environment of čapek-dogsz initially has pH 6.0. Acidification is carried out before autoclaving it with a 0.1Ν HC1 solution. II 1514764 12 Table 7 Continuation of table 7 The coolant on the offer · ” five my and known environments Naimova Coolant niya Evaluation mushroom vitality from coolant to Name Viability assessment Coolant mushrooms from coolant in Proposed Famous environment Cha's environment PPRPYAGYARMPY Famous ten peca-doxa environment Cha's environment Through Through Through Through pitch- -Dox 18 h ί 24 h 18h [24 h Through Through Through Through - --- G . - four 18 h 24 h 18 h 24 h 15 UKPINPP 1M Autocat 3 four 0 0 (after filter Autocat 2 four 0 0 · walkie talkies) four - 0 0 Ukrinol 1M 0 0 0 0 Carbamol 111 2 - 0 0 Ukrinol 1M* 0 0 0 0 Ukrinol 1M 0 0 0 0 Tosol OIZ 0 2 0 0 20 'Avtokat 0 0 0 0 Ukrinol 1M 0 0 0 0 Autocat 0 0 0 0 Tosol OIZ one four 0 0 Ukrinol 1M 0 0 0 0 Ukrinol 1M 0 0 0 0 Ukrinol 1M 0 0 0 0 Ukrinol 1M 0 0 0 0 25 Ukrinol 1M 0 0 0 0 Note: * - SOZH Ukrinol 1M may- Tosol OIZ 0 0 0 0 lo-emulsion Autocat 0 0 0 · 0 like Emulsion be- Autocat 0 0 0 0 barking opaque. Autocat (about - thirty ** _ Coolant Tosol OIZ syn- would be taken from tetic type asterisks gives true pro dredge with foam) 0 0 0 0 clear solution. Carbamol 111 * * 4 - 0 0 ** * - Coolant Carbamol 111 Ukrinol 1M 35 semi-synthetic (before filter- type Emulsion 4) - 0 0 translucent.