SU1509730A1 - Apparatus for scanning thin-layer chromatograms - Google Patents

Abstract

This invention relates to devices for scanning thin-layer chromatograms. The aim of the invention is to simplify and speed up the processing of results. The device is additionally equipped with a light source and a line of photodiodes connected to an analog-to-digital converter, storage and computing devices. The plate moving speed is synchronized with the operation of the storage device by connecting its address inputs to the outputs of a pulse counter, which through an amplifier is connected to the output of a light receiver fixedly relative to the light source and the line is additionally equipped with perforations of the chromatographic plate. 1 il.

Description

The invention relates to thin-layer chromatography, in particular to scanning means for processing the results.

. The purpose of the invention is to simplify and accelerate the processing of results.

The drawing shows schematically a device for the automatic detection of chromatograms in a thin layer of sorbent.

The device contains a chromatographic plate 1 with substances separated on it, a line of light sources 2, a line of photodiodes 3 (only one light source and one photodiode are shown in the diagram), analog-to-digital converter 4 memory device 5 with information 6 and address 7 inputs, computing device 8, an additional light source 9, such as an LED, and

an additional light receiver 10, for example a photodiode, an amplifier 11 and a pulse counter 12. There is a perforation 13 at the edge of the chromatographic plate. The perforation can be done. the yen both on the chromatographic plate itself and on a specially made frame into which this plate can be inserted, or on a board to which this plate can be attached.

The device works as follows.

By moving the chromatographic plate 1 along guides (not shown), the intensity of the light reflected from the plate varies depending on the location of the zones of the separated components, which causes a change in the current of the photodiode 3 bar. This current is converted into binary code in the ADC 4.

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When separating several mixtures on one plate, as well as in the case of multidimensional chromatography, several ADCs can be used as an ADC for each mixture (channel), or one Li.P with dynamic polling of each channel. The binary code from the ADC outputs goes to the information inputs: 6 ZU 5 At the same time, light pulses 5 formed by adding 9 amp light and perforations 13, get to additional light receiver 10, causing the appearance of photocurrent pulses at the input and voltage pulses output of the amplifier 11. As a result, a binary code corresponding to the hole number of the perforation 13 appears on the pulse counter of the pulse counter 12. This code goes to the address inputs 7 of the charger 5 and is the address of the cells in this memory. Current information, attending 3 present the information input 6

Thus, the real xp-phonogram is divided into discrete values, which are memorized; 1 st in the corresponding memory cells, regardless of the speed of the plate moving. The number of these X rates is equal to the number of perforation holes on the plate. Then, similarly to that described, it is possible to perform detection of another chromatographic plate, while all information is recorded in the following cells of the charger 5. With the help of the computing device 8, analyze

improved) To the results for a given program. A microprocessor kit can be used as a computing device.

Lack of precision mechanical parts; complex structural elements, as well as the ability to use any chromatographic plates, makes this device cheaper; BbMs are easy to manufacture and reduce maintenance requirements.

Claims (1)

Claims. A device for scanning thin-layer chromatograms, an adjoining drive, a light source and a line of photodiodes; the outputs of which are connected in series with an analog-digital transducer five 0 oramer. and a memory device, a vychiklkoy device, that is, so that, in order to simplify and speed up the processing of the results, it is equipped with an amplifier, a pulse counter, an additional source of light, and an additional light receiver, connected to the input of the Usggitap g whose output is connected to the input of the pulse counter, the outputs of which are connected to the address inputs of the memory device ;. The information inputs of the latter are connected to the outputs of a known-diffraction converter, while on the chromium then the graphic plate there are perforations along one of its edges.