SU1501129A1 - Method of simulating endogenous intoxication - Google Patents

Abstract

The invention relates to medicine and is intended to study the pathogenesis and morphogenesis of endogenous intoxication arising under the influence of products of natural metabolism. The aim of the invention is to increase the reproducibility of the model. A mixture of aqueous solutions of lactic acid (330 mg / kg), ammonium chloride (116 mg / kg), urea (3200 mg / kg) and uric acid (68.5 mg / kg) was intraperitoneally administered once to the animal. These compounds are natural metabolites and, when taken in low toxic doses, potentiate each other in a mixture, resulting in a lower level of mortality, which makes it possible to study the development of endogenous intoxication over time. 1 tab.

Description

The invention relates to medicine, namely to general pathology, and is intended to study the patho- and morphogenesis of endogenous intoxication arising under the influence of products of natural metabolism.

The purpose of the invention is to increase the reproduction of the method.

The method is carried out as follows.

Once a day, a mixture of water solutions of lactic acid 330 mg / kg, ammonium chloride 116 mg / kg, urea 3200 mg / kg and uric acid 68.5 mg / kg body weight, which corresponds to 1/2 DC, for each of listed components, with the specified route of administration.

The toxicity values found for each of the components were determined by the method of Litchfield and Wilcoxon, followed by the construction of a dose-dependent direct toxicity and are presented in the table.

Substance

3150

Unlike carbon tetrachloride, these compounds are products of natural metabolism and, when taken in low toxic doses, potentiate the effects of each other in a mixture, causing dystrophic and necrobiotic changes, resulting in impaired physiological functions of organs and systems.

Example. Lactic acid, 330 mg / kg, ammonium chloride, 116 mg / kg, urea, 3200 mg / kg, uric acid, 68.5 mg / kg body weight, which is 1/2 DC, is given to the animal, indicating that the toxic potentiation is action of the above mixture components.

Pathological studies of the liver, kidneys, and lungs of the heart were carried out. Microscopic examination of review preparations stained with hematoxylin and eosin was found: in the kidneys - in the glomeruli - aggregates of erythrocytes with the formation of microbthromes, focal hemolysis, hyperemia loops and their segmental fibrinoid necrosis. The epithelium of the proximal tubule is proteinaceous, here and there hyalino-droplet. On dystrophy with foci of desquamation of the epithelium and destruc- . In the stroma of the kidney, there is a pronounced plethora of small veins, small focal hemorrhages, interstitial and perivascular swelling, in the liver there is plethora and dilation of sinusoids, sometimes beams are compacted, microtromes, and sometimes protein precipitate. In protein hepatocytes, in places hydropic dystrophy

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in the lungs - disttelectasis foci expressed by the hyperemia of the vascular bed, red blood cell sludging. In the lumen of the veins - microthrombosis, in the heart - interstitial edema, capillary hyperemia. Myocardiocytes - focal proteinovy dystrophy, fragmentation and myolysis, hypochromia and vacuolization of the der. Other internal organs showed similar changes, accompanied by dystrophy of cellular elements and microcirculation disorders.

Based on the foregoing, it can be concluded that severe dystrophic and focal necrobiotic changes are found in the internal organs, as a result of pronounced microcirculation disorders, which appear during the modeling of endogenous intoxication with natural metabolites.

These natural metabolites, when administered together, mutually potentiate their toxic properties, leading to a lower level of fatality (compared to the prototype), which makes it possible to study the development of endogenous intoxication over time.

Claims (1)

Formula from the range A method of modeling endogenous intoxication by exposing toxic substances to an experimental animal, characterized in that, in order to increase the reproducibility of the model, aqueous solutions of lactic acid in a dose of 116 mg / kg of ammonium chloride in a dose of 116 mg / kg, urea in a dose of 3200 mg / kg and uric acid at a dose of 68.5 mg / kg.