SU1474722A1 - Method for simulating of torsion dystonia - Google Patents

Abstract

This invention relates to neurology, neuropathophysiology, and can be used to modulate torsion dystonia. The aim of the invention is to increase the reproducibility of the method. The method is carried out by introducing into the cortex the cerebellum of kainic acid in a dose of 0.5-1.0 µg. Electrostimulating zones of drug administration with a frequency of 100-300 Hz, with a voltage of 5-10 V for 20-30 s.

Description

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The invention relates to the field of medicine, in particular, neurology and neuropathophysiology, and can be used to simulate torsion dystonia.

The aim of the invention is to increase the reproducibility of the method.

The goal is achieved by injecting 0.5-1.0 µg of kaic acid into the cortex of the worm of the cerebellum, and then after 30-60 minutes, this zone is irritated by an electric current of 100-300 Hz voltage of 5-10 V for 20 -30 seconds

The method is carried out as follows.

An animal (cat, rat) under nemutin (40 mg / kg) or hexenal (100 mg / kg) anesthesia is implanted with irritating electrodes into the bark of a worm, and a cannula tip is placed between the electrodes. Che

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10–14 days after surgery, 1.0 µg of kainic acid was injected with a cannula in a volume of 5.0 µl of the buffer solution, and 30–60 min after the introduction, they were subjected to electric current (100–300 Hz, 5–10 V) for 20-30 s. Changes characteristic of torsion dystonia are observed: slow voluminous rotational movements of the extremities, turns along the longitudinal axis of the body, involving torso muscles or neck muscles, as well as a combination of these changes, changes in the position of the body, tremor of the extremities. These changes are observed both directly during the period of irritation with electric current, and within 5-10 minutes after its cessation. Symptoms of torsion dystonia in the above volume were easily reproduced in all experimental animals during and after

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Example 1 A rat weighing 270 g under Nembutal anesthesia implanted irritating electrodes into the cortex of the black cerebellum (U-lobule), Adrian's concentric electrodes into the digastric and triceps muscles of the shoulder and also implant the cannula into the paleocerebellum cortex between the irritating electrodes. 10 days after surgery, 1.0 µg of kainic acid was injected in a volume of 2.5 µl of buffer solution through a pre-implanted cannula using a microinjector. 30 minutes after the administration, the cortex of the paleocerebellum is subjected to high-frequency (100 Hz) electric current (5.0 V a for 30 s). The development of symptoms of torsion dystrophy, athetoid-like movements of the trunk, limbs, tremor of extremities characteristic of torsion dystonia is noted. simultaneous contraction of the aitagonist muscles. These changes continue for 6 minutes after the cessation of irritation. Thus, acute torsion dystonia has been reproduced in the squat.

PRI mme R 2. A 300-rat under kembutal anesthesia is implanted with irritating electrodes in the region of the cerebellar cortex (U-lobule), the concentric electrodes in two and

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the cannula is planted into the paleocerebellum cortex between the irritating electrodes. 13 days after surgery, 1.0 µg of kaic acid was injected in a volume of 5.0 µg of buffer solution through a pre-implanted cannula using a microinjector. 30 minutes after the administration, the cortex of the paleocerebellum is exposed to high-frequency (300 Hz) electric current (10V) for 20 s. Developmental symptoms of torsional dystonia of position-tonic disorders, athetoid-like movements of the trunk, neck, and extremities of a rotational nature, as well as tremor, are noted. When studying the myogram of antagonist muscles, a simultaneous contraction of these mice is noted. These changes are noted within 8 minutes after cessation of irritation. Thus, acutely forming torsion dystonia syndrome has been reproduced in the animal.

Claims (1)

Invention Formula A method of simulating torsion dystonia by stimulating brain structures, characterized in that, in order to increase the reproducibility of the method, kartic acid worm is introduced into the cortex of the cerebellum in a dose of 0.5-1.0 µg, then after 30-60 minutes electro-stimulation is performed zones of drug administration with a frequency of 100-300 Hz, voltage 5-10 V for 20-30 s.