SU1465453A1 - Method of producing molybdenum from diluted aqueous solutions - Google Patents

Abstract

The invention relates to biotechnology, the extraction of molybdenum from dilute aqueous solutions using microorganisms, and can be used in the production of metals by hydrometallurgical, as well as in the treatment of industrial wastewater. The aim of the invention is to simplify and cheapen the method for extracting molybdenum from dilute solutions. The method consists in introducing into the cleaned solution containing molybdenum at its initial concentration of 250-100 mg / l, previously consumed biomass of microorganisms, by stirring the solution for 10-20 minutes at a pH of 1.0-2.0. Biomass is separated by filtration or centrifugation, and molybdenum is extracted, desorbed with water at pH 6.0-1.0, or incinerated biomass. The method makes it possible to extract molybdenum by 90-100%. 1 tabl .. about S L

Description

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The invention relates to fatiotexho- logia, namely, to a method for producing molybdenum from dilute aqueous solutions using microorganisms, and can be used to obtain hydrometallurgical metals at the Institute of Metals, as well as in the treatment of industrial wastewater.

The aim of the invention is to simplify and cheapen the process for producing molybdenum from dilute solutions.

The method is carried out as follows.

Pre-grown biomass of microorganisms is introduced into an aqueous solution containing molybdenum at its initial concentration of 250-100 mg / l. The mixture is stirred for 10-240 minutes at pH 1.0-2.0 and ambient temperature. Biomass is separated by filtration or centrifugation, and molybdenum is extracted, desorbed with water at pH 6-10 or incinerated biomass. The biomass can be reused many times, while its sorption capacity is fully preserved.

Microorganism cultures are used — microscopic fungi: M.thermophia. Asp. terreus. Asp. niger, yeast - Candida valida, Soccharomyces.

The recovery rate of molybdenum is 90-100%.

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Molybdenum content in solution Determined by the rhodanide method using a calibration curve, using thiourea as a reducing agent. The amount of adsorbed molybdenum is determined by the difference between its initial amount and rstavim in the solution after adsorption, as well as by direct determination of adsorbed on the bio-mass of molybdenum.

In acidic pH 1, 0-2.0, almost all molybdenum (95-100%) is sorbed on the biomass of microorganisms. At pH 2.0, there is a sharp drop in sorption capacity. Full desorption of molybdenum ions and biomass occurs at pH 9.0. A decrease in ipH of 6.0 results in a decrease in the desorbed metal by more than 50%.

The duration of contact between plant and JBOpa molybdenum and biomass, which is necessary for maximum sorbhii of the metal, depends on the types of microorganisms. The biomass of the sorbic fungus cultures is the maximum amount of molybdenum for 240 minutes and a further increase in the contact time has practically no effect on desorption. Bacteria adsorb it for 100% in the first 10 minutes, yeast - 60 minutes.

Example 1. Preparing a liquid nutrient medium, g / l: NaNO 1,

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| KN, P04

1, jO 0.5-, corn extract 1, sucrose 20, tap water, pH 6.0. Poured into a count of 1 Erlenmeyer in 250 ml of 100 mp in the kaidzu and sterilized at 0.5 atm. I Sow the spores of the fungus Myceliophtho-; ha thermophila (VKM-2148 D). At a speed of 180 rpm, it is cultivated during the day at 40 ° C. Biomass is separated by filtration. I flasks of 250 ml are filled with I, 5 g of biomass (350 mg completely dry) and 50 ml of solution. Ammonium molybdate with a molybdenum concentration of 250 mg / l at pH 2.0 and contacted with stirring for 240 minutes. The biomass containing molybdenum is separated and the amount of molybdenum adsorbed is determined. On the biomass of the fungus M. thermophila is sorbed from a solution of 92% molybdenum or 33 mg Mo / g of absolutely dry biomass. The desorption of molybdenum to an eGHDL-, placing biomass in water, alkalized to pH 6.0, with stirring for 30 minutes. The biomass is separated by filtration and reused 14654534.

for the next molybdenum adsorption. Molybdenum is desorbed from biomass at 100%.

Example 2. Get biomass-. Su M. thermophila according to Example 1. In a 250 ml flask, 1.63 g of biomass (168 mg completely dry) and 50 ml of ammonium molybdate solution with 10 mM / l molybdenum concentration at pH 2.0 were added. In 60 minutes of contact with biomass, it is sorbed from a solution of 60% molybdenum or 181 mg Mo / g of biomass. The desorption process is carried out according to Example 1. Molybdenum is desorbed to 100%.

Example 3. Aspergillus terreus biomass (VKM F-1862) was prepared according to Example 1. 1.5 g of biomass (294 mg dry weight) and 50 ml of solution with an initial molybdenum concentration of 250 mg / l were added to 250 ml flasks, pH 2.0. During the 240 minutes of contact with the biomass, with stirring, 92% molybdenum or 25 39 mg Mo / g of absolutely dry biomass was absorbed from the solution. Molybdenum is desorbed in Example 1 at pH 10.0 at 100%.

Example 4. Get biomass Asp. terreus according to example 1. 30.,

 in flasks of 250 ml., 1.82 g of biomass (349 mg on a dry weight) and 50 ml of a solution with an initial molybdenum concentration of 500 mg / l at pH 2, OO 90 ml of molybdenum are absorbed during mixing with biomass ( 65 mg Mo / g dry biomass). Desorbable molybdenum of Example 1 at pH 8.0 per 100%.

Example 5. Get biomass Asp. niger according to example 1. 1.5 ml of biomass (356 mg on a dry basis) and 50 ml of solution with an initial concentration of 45 molybdenum 250. mg / l, pH 2.0, are introduced into 250 ml flasks. After 60 minutes of contact with the biomass, it was sorbed from a solution of 90% molybdenum (32 mg Mo / g dry biomass). The molybdenum in Example 1 was desorbed to 100%.

Example 6. Preparing a liquid medium, g / l: NaNO, 3 KN, 2, MgSO 0.5, malt sprouts 2.0, tap water pH - 5.0. Poured into Erlenmeyer flasks of 250 mp 100 ml, sterilized at 1 atm. Spores of M. therraophila are infected with spores. Cultivated when swinging 180 rpm for 2 days. Division of biomass prices trifugirrvanie em

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0.8 g of biomass (200 mg dry) and 50 ml of ammonium molybdate solution with a molybdenum concentration of 250 ml / l at a pH of 2.0 are introduced into 250 ml flasks and contacted with stirring for 240 minutes. The amount of adsorbed molybdenum is determined. 100% molybdenum (75 mg Mo / g biomass) is sorbed on biomass.

Example 7. Preparing a liquid medium, g / l: NaNO, 3, KHj, P04 2, MgSO 0.5, corn extract 5; filter paper 20, tap water 5 pH 5.0. Grow and get biomass and in example 6,

1 g (283 mg of dry pulp) and 50 ° MP of ammonium molybdate solution with a molybdenum concentration of 250 mg / l at pH 2.0 are injected into 250 ml flasks, for 240 minutes. The amount of adsorbed molybdenum is determined. 100% molybdenum V is adsorbed on biomass.

Example 8. Preparing a Reader liquid medium, g / l: (NH4) 2.S04 3; tfeSO 0.7; NaCl 0.5, KN, g104 1.0; 0.1; ethyl alcohol 10; yeast extract 1; tap water, pH 6.5. , Poured into 250 ml Erlenmeyer flasks each, sterilized at 1 atm. Ethyl alcohol and (in the form of a sterile solution) are introduced into the flasks after sterilization. Candida valida yeast VKM-247 cultured on wort agar was seeded with culture (one suspension was seeded with 5 flasks.) Cultivated at 180 rpm for 72 hours at 28 ° C. Biomass was separated by centrifugation. In 250 MP flasks, 0.5 g of raw biomass (75 mg, calculated on dry weight) and 10 ml of ammonium molybdate solution with a molybdenum concentration of 250 mg / l at pH 2.0 are added and contacted. for 10 minutes The biomass containing molybdenum is separated, and the flow of molybdenum is determined in solution. 90% of molybdenum or 16 .mg Mo / 1g of absolutely dry biomass is absorbed from the solution on the biomass. Example 1 is desorbed to 100%. Example. The biomass of bacteria Bac.coagulaus VKM-949 is obtained on a mineral medium, g / l: 0.2, MgS04 0.2i NaCl 0.2; Caso, 2; (N114) 4804 1, yeast extract 1.0, sucrose 10.0; vodopro

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water; pH Poured into 250-mp Erlenmeyer flasks of 40 mp each, sterilized at 1 atm from 20 minutes. Sow culture grown on kartsfelno-peptone agar, g / l: peptone 5, chalk, 1; yeast extract 1; 20% potato decoction; pH 7.2.

10 Cultivate by rocking at 180 rpm for 72 hours at 60 C. feoMaccy is separated by centrifugation. About 250 ml of O flask are added to the flasks, 1 g of the raw biomass of bacteria (14 mg in terms of

15 dry weight) and 10 MP of ammonium malibdate with a molybdenum concentration of 250 mg / l at pH 2.0 and contact with stirring for 60 minutes. The biomass is separated and the remaining molybdenum is determined in solution. On biomass 84% of molybdenum or 150 mg Mo / 1 g of absolutely dry biomass is absorbed from the solution. Desorbed in example 1 to 100%.

25 Example 10. Biomass Bac.coagulaus-165 is obtained according to Example 9.

0.2 g of 30 raw bacterial biomass (30 mg in dry weight) and 10 ml of ammonium molybdate with a molybdenum concentration of 250 mg / l at pH 2.0 are added to 250 ml flasks. After 10 minutes of contact, 100% molybdenum is sorbed (83 mg Mo / 1 g dry biomass). Desorbed on. Example 1. The degree of desorption is 100%.

Examples 11-24. The data in examples 11-24 are summarized in table 1.

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Examples 11-15 are carried out according to Example 2; examples 16-20 - according to example 5, only biomass is obtained on agar wort; examples 21-23 - according to example 5, only biomass is obtained on potato-peptone agar; Example 24 - in Example 5, only biomass is obtained on MPA. As can be seen from the table, the biomass of various microorganisms is able to sorb molybdenum.

In the proposed method of extracting molybdenum from diluted gg solutions, microorganisms are used, drip-. in a short time (1-3 days, 10 times faster than in the known method) under aerobic conditions, large biomass on simple composition and cheap synthetic media, which are used in industry to produce BVK, antibiotics, enzymes and other biologically active compounds. Biomass of cellulose-destroying fungi Asp. terreus and Muceliophtora thermophila is a waste in the production of cellulases, and biomass Aspergillus niger in the production of citric acid. Biomass is easily separated from the culture fluid and can be directly used to extract molybdenum.

Claims (1)

The invention The method of obtaining molybdenum from dilute aqueous solutions, which involves contacting the biomass of microorganisms with the treated solution under optimal first conditions to the maximum sorbda and metal, followed by its extraction, in order to accelerate, simplify and reducing the cost of the method; a previously expanded culture of microorganisms is used as biomass and it is contacted at a pH of 1.0-2.0. Bisorbcy Mo cells of various microorganisms