SU1459656A1 - Method of diagnosis of toxemia - Google Patents

Abstract

This invention relates to the diagnosis of toxemia. The aim of the invention is the early diagnosis of the disease. The washed erythrocytes of the examined patient are incubated for 15 min with aniline naphthalene sulfonate, the precipitate is separated by centrifugation, a 1% solution of Triton X-100 is added to the supernatant, measured on a fluorimeter, and the amount of bound aniline naphthalene sulfonate with erythrocytar is determined paste the patient. At a value of 0.875 nmol per 1 million erythrocytes and above, toxemia is diagnosed.

Description

one

The invention relates to medicine, namely to the diagnosis of toxemia, and can be used to detect and determine the toxicity of substances causing the syndrome of endogenous intoxication, as well as to evaluate the effectiveness of detoxification therapy.

The aim of the invention is the early diagnosis of the disease.

Example 1. Red blood cells washed by an isotonic sodium chloride solution of the patient under study (0.5 ml erythrocyte paste) are incubated for 15 seconds with 0.5 ml of a solution containing 8 X XYu® mol 1.8 aniline naphthalene sulfonate (1.8 ANS) , centrifuged for 2 minutes at 8000 th-rpm, 0.5 ml of supernatant was taken, 2.0 ml of 1% aqueous solution of Triton X-100 was added, measured on a fluorimeter (recovery 365 nm, Lregistr. 465 nm) the amount of unloaded 1.8 ANS against a solution containing 0.5 ml of the initial solution 1.8 ANS and 2.0 ml of 1% aqueous solution of Triton X-100, by calculation l the amount of bound ANS 1.8 and the last index in excess of the limit of 0.875 nmol / million diagnosed toxemia

z- ()

l f. /

BUT, ,

where L, - the number of Holy Zavshagos 1.8 ANS,

(L

(

nmol 1 million er.

)

Z

li

- original amount, nmol;

-relative fluorescence intensity over time t

1 - relative fluorescence intensity of the control; E - the number of red blood cells in 1 mm

million

Data taken as the norm was obtained during the examination in terms of clinical examination of eight healthy people - 0.477: t2X

X0.199 -. million

The group of patients studied consisted of 15 people with the syndrome of endogenous intoxication on the basis of hepatocerebral insufficiency (liver tumor, exacerbation of chronic active hepatitis) - 2; diffuse peritonitis (perforated stomach, gangrenous appendicitis) - 4; sepsis - 2;

four

sd

with

C5

sd

about

acute suppurative lung diseases complicated by pleural empyema - 3; burn disease - 1, post-resuscitation disease - 1, hepatorenal failure (mechanical jaundice, poison death by the toadstool) - 2; The average number of CVs of 1.8 ANS in this group is 1.432 ± 0.054, which is significantly (, 001) different from the norm.

The table presents the survey data of a group of patients with endogenous intoxication syndrome.

The average in the group of patients studied with endogenous intoxication syndrome (1.432 + 0.054) was significantly different from normal (, 64,, 001).

Example 2. Bg. J., was admitted to hospital for residual effects of acute abscess of the upper lobe: patient E. - exacerbation of chronic pneumonia on the basis of bronchiectasis. The data of clinical, biochemical and immunological blood tests did not allow objectifying the diagnosis of toxemia, despite the presence of clinical signs of intoxication in patient J., the data of the listed blood tests were identical. The study of the sorption of the ANS probe on the surface of the erythrocyte membrane according to the proposed method gave the following indicators:

patient J. 1,307 nmol / million cells, in a patient with E-0.817 nmol / million. cells, which made it possible to objectively expose the patient J. along with the main diagnosis of endogenous intoxication syndrome and carry out appropriate detoxification therapy, which resulted in a significant improvement in the patient’s condition, which is also reflected in the sorption indicators of the ANS probe according to the proposed method -

one week after starting treatment, 0.628 nmol / million. cells.

Thus, according to well-known clinical tests, early diagnosis of toxemia is difficult, while at the same time

The method of fluorescent probing of erythrocyte membranes allows objectively and with sufficient accuracy to diagnose toxemia in the early stages of its development.

20

Claims (1)

Invention Formula A method for diagnosing toxemia by determining blood biochemical parameters, characterized in that, in order to diagnose the disease early, the amount of aniline naphthalene sulfonate with erythrocyte paste of the patient is examined and, if this indicator is 0.875 nmol, per 1 million erythrocytes and higher, toxemia is diagnosed. D. R. L. BUT. G. WITH. InLiltrative pulmonary tuberculosis in the phase of decay and contamination, right-sided spontaneous pneumothorax1 552 Acute abscess of the left lung, pleural empyema is total, bronchial fistula 1,371 Right nonspecific empyema of the pleura, condition after splenectomy, liver closure, drainage of the subdiaphragous abscess 1,252 Post-reanimation disease, condition after pulmonectomy for hypoplasia of the left lung, intrapleural empyema1,330 Vascular endarteritis obliterans lower limbs, sepsis1,430 Diffuse peritonitis, perforated stomach 1,344 Diffuse peritonitis, gangrenous perforated appendicitis1,583 6 Table continuation