SU1440916A1 - Strain of arthrobacter flavescens bacteria for purifying waste water from sulfurous compounds - Google Patents

Abstract

The invention relates to the microbiological industry and relates to a new bacterial strain used to purify sulphide-containing wastewater from ash factories of leather factories, stratal waters of serum mines, wastewater from tissue dyeing in textiles, balneological resorts. The aim of the invention is the bacterial strain Arthrobacter flavescens VKPM B-3496, capable of pinching and oxidizing higher concentrations of sulfur compounds at a higher rate. The strain is able to withstand and oxidize sulfides to thiosulfate. concentrations above 1.5 g / l at pH 11.0-11.5 with an average oxidation rate of 300 mg / lh. 1 that bl. i (L 4ib 4a; about 9

Description

The invention relates to the microbiological industry and concerns a new bacterial strain used to clean sulphide containing soffax libix water of the ash workshops of kozhzanods, stratum waters of sulfur mining mines, textiles in the textile industry in balneological resorts, to

The purpose of the invention is a strain of bacteria rthrobacter flavescens, capable of sustaining and oxidizing higher

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concentrations of sulfur compounds with greater speed, 15

The strain was transported by autoselling from a bioreactor biofilm with a weighted loading layer with it, and microflora fixed to it, adapted to high concentrations of sulfur compounds. The adaptation of biogasks was carried out by gradually increasing the load on the bioreactor of sulfur compounds, in particular (8-G6 g NdZ / LoSut) .25

The strain is stored in a column of semi-liquid agar at 4 s for 2-3 months1. In order to preserve oxidative activity with sodium sulfide every two to three months, the culture must be transplanted onto Mllai plates on which then paper blocks are impregnated solution with a concentration of 250-500 mg / l. After 7 days of storage in plates of 35 kamie-soaked, the culture is again seeded for long-term storage in semi-folding nutrient agar tubes.

Strain A, flavescens deposited 40 in the All-Union collection, industrial microorganisms under the number VKPM B-3496 and has the following characteristic;

Cultural and morphological signs. Polymorphic mobiles: dad- dads ranging in size from 0.7 to μm, the transition w 1e to cocci with a diameter of about 0–35 μm depending on the stage of development. Propagation by transverse de-zation does not form spores. Gram-positive, non-acid-resistant.

When seeding on nutrient agar, Petri dishes after 18-24 hours form round smooth, slightly convex, ligated colonies with smooth edges. The diameter of the colonies 2-4 mm. The pigment color is orange (from light orange

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go to juicy orange). When sowing nutrient agar column growth is strictly in the upper part of the agar, In the broth give weak diffuse cloud.

Chemoorganotrophs with respiratory metabolism, Catalosopositive. Hugh Leorson glucose is not fermented. The starch is decomposed, the gelatin cleaved. Cellulose is not cleaved. Indole do not form. Restore. nitrates and nitrites,. The strain grows in a wide range of pH and is able to withstand highly alkaline conditions caused by the presence of Na2S in wastewater. Strain A, flavescens VKPM B-3496 can purify wastewater with a sulfide concentration in the original wastewater over 1.5 g / l and hold the pH in the bioreactor is 11.1-11.5. Thus, the range of pH values at which the strain vitality occurs is 7 pH 11.5.

The optimum temperature conditions for the development of the strain are 20- ..- When the temperature drops to 15 ° C or the temperature rises to 40-50 seconds, the oxidative activity of the strain drops by half.

Strain - strict aerobic „The most effective oxygen concentration in the bioreactor is 1-3 mg / l.

For example, Biomass of the strain is increased by MPa in a thermostat at 37 ° C for 3 days. The obtained biomass is used to purify the wastewater of the ash workshop at the tannery with a sodium sulphide concentration of 2283 mg / l (in terms of hydrogen sulfide). The oxidation process is carried out in two successively located bioreactors with a fluidized bed of sandy load,

The strain is introduced into the first bioreactor in an amount of 0.1 g / l (by dry weight). The duration of aeration was 6.9 h, - pH 11.0, temperature, average oxidation rate 297.3 mg / l "h. Water after the first bioreactor, containing mg / l sulphides and oxidized sulfur compounds, mainly represented by thiosulfates, enters the second bioreactor, into which 0.1 g / l (known as dry matter. Thiobacillus tbidparus) is introduced. aeration 2.3 hours Pa from the entrance 31144091

No bioreactor was found in water. Purified water is discharged to the city sewage treatment plant.

Thus, the average oxidation rate of hydrogen sulfide in the second stage is 10b mg / l, the total treatment time is 9.2 hours, the degree of water purification from hydrogen sulfide is 100% .10

The test results of the oxidative activity of a new strain of A. flavescens VKPM B-ZA96 compared to the known are presented in the table.

The results indicate that using the BCS strain B-3496, a high oxidation rate of sulfide to thiosulfate can be achieved, while the strain is able to withstand and oxidize sulfites at a concentration of over 1.5 g / l at pH 11.0-11 ,five.

Claims (1)

Invention Formula The strain of bacteria Arthtobacter flavescens VKPM B-3496 for the purification of wastewater from sulfur compounds.