SU139876A1 - The method of obtaining labeled on the floor of silkworm hybrids yes - Google Patents

Description

The supereducational genetic labeling of silkworm eggs on the floor by autosome transplantation has long been known. However, the task of industrial use in the production of only males was not provided. This is due to the fact that, when marking males for the mutant white trait, the grenes decreased the viability of the males at the embryonic stage of their development. Decreased viability did not allow recommending male white grene for industrial feeding.

The proposed method does not have this disadvantage due to the creation of breeds labeled by sex, combining precise sex separation of breeding material and high viability of silkworm hybrids.

A distinctive feature of the proposed method for obtaining sex-labeled hybrids of silkworm at the egg stage is that two translocated labeled sex-based breeds resolve. These breeds with similar external, but non-allelomorphic recessive signs of coloration of male creeps have a zero percent overlap.

As a result of this crossing, both mutations become heterozygous. As a result, the signs caused by mutations are normal. At the same time, the color of the greens in male hybrids becomes, instead of white, light brown, and the female embryo egg still maintains a dark color. Dividing such a green by the floor gives an error of 1.4%. The viability of caterpillars of light brown greny is 77.8% versus 70.2% of the viability of caterpillars from dark eggs of the same origin (/ tf 0.998).

FIG. Figure 1 shows the gren of two translocant lines (W and 4). Dark eggs are female, whites are male.

AO 139876-2 In FIG. 2 - hybrid of green. Dark eggs are female, light brown ones are male.

For the implementation of the method, there is a silkworm line (with white color, grains and eyes are crossed with another mutant line, in which the pigment is absent not only in the green and the eyes, but also in the skin of the caterpillar (. When crossing these lines, the mutations turn into heterozygous state (W-W WzWft} The scheme of industrial use of sex-labeled alkaline rocks is that the circular cocoon rocks intended for industrial hybridization must have as a male of white greens and eyes and normally pigmented Skin: Breeds with sturdy cocoons — white grains and eyes and transparent skin.In the first stages of breeding, the potentially slightly reduced viability of white-eyed males does not have any economic value. males of round breeds and females of elongated females must be fed. Thanks to this, feeding of less viable transparent males is excluded.

An industrial hybrid lawn between the two translocant lines can be accurately divided by the floor by automata working on photovoltaic cells and transfer to the industrial fertilizing plants only male.

The subject is invented and

The method of obtaining sex-labeled hybrids of the silkworm and egg stages, characterized in that, in order to increase the viability of these hybrids and more accurately divide them by sex, two translocated labeled sex-breed breeds with external similar, but non-allelomorphic, recessive signs of the color, male sex, having a zero percent overlap.

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