SU1393430A1 - Method of sterilizing a container - Google Patents

Description

(21) 4065603 / 28-14

(22) 03/19/86

(46) 05/07/88. Bul Number 17

(71) Omutninsky Chemical Plant

and All-Union Scientific Research Institute of Applied Biochemistry

(72) E.L. Agafonov, V.A. Bystrykh, Yu.V. Agafonov and N.N. Sidorov

(53) 615.777 / 779 (088.8)

(56) USSR Author's Certificate No. 865296, cl. A 61 L 2/06, 1979.

(54) METHOD OF STERILIZING CAPACITY

(57) The invention relates to sanitation and can be used in medicine and microbiology for sterilizing fermentation equipment. The purpose of the invention is to prevent re-seeding of the container with extraneous microflora. For this, an autoclave with an internal volume of 200 liters is exhausted with fluid steam at a pressure of 1 kg / cm. When heated for 25 minutes, saturated steam is supplied to the autoclave, and the sterilized fermenter is supplied with compressed air at a pressure of 2.7 kg / cm and the temperature of the steam is 130 ° C. The temperature of the walls of the fermenter is also at the end of heating. The saturated steam pressure in the tank is 0.1–0.5 kg / cm less than the pressure of the saturated steam in the tank. After the tempering pressure, the vapor pressure in the autoclave and the fermenter is dumped with 1D atmospheric pressure and the condensate is drained from the autoclave.

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The invention relates to sanitation and can be used in the microbiological, medical and other industrial areas for sterilizing fermentation devices.

The purpose of the invention is to prevent re-seeding of the container with extraneous microflora.

Example. In a 200-liter autoclave (chamber) with an internal volume of 200 liters, a 100-liter metal fer- MeHTatop is sterilized. Pre-press air from the autoclave by flowing steam at a pressure of 1 kg / cm -. When heated for 25 minutes, saturated steam is supplied to the autoclave, and compressed air is fed into the fermenter at a pressure of 2.7 kg / cm. The temperature of the steam is 130 ° C at the end of heating.

After a 3-minute additional depressurization of the autoclave and the fermenter, the process of the pH: holders is carried out until atmospheric and the condensate is drained from the autoclave. At the same time, for 20 minutes, the saturated steam is fed to the fermenter at a pressure of 2.65 kg / cm and a temperature of 129 ° C, and into an autoclave at a pressure of 2.6 kg / cm and a temperature of 128 ° C. Thus, the saturated vapor pressure in the fermenter is 0.05 kg / cm less than the vapor pressure during heating, and the pressure in the autoclave is 0.05 kg / cm less than the pressure

saturation in the fermenter. At the same time, the sterilization criterion inside the fermenter is 39. After the temperature temperature, the vapor pressure in the autoclave and the fermenter is discharged to atmospheric and the condensate is drained from the autoclave.

The proposed method allows to increase the reliability of the sterilization process and to ensure that the requirements for equipment operation under aseptic conditions are met.

Claims (1)

5 claims five The method of sterilization of the tank by heating with saturated steam in the chamber, temperature exposure and subsequent reduction of pressure to atmospheric, characterized in that, in order to prevent re-seeding of the tank with extraneous microflora, before heating, the tank is supplied with compressed gas under saturated vapor pressure and in the tank, while the temperature exposure is carried out with simultaneous supply of saturated steam into the chamber and the tank at a pressure of saturated steam in the tank by 0.1-0.5 kg / cm less, than vapor pressure when heated, and the pressure in the chamber to 0.1-0.5 kg / cm less than the vapor pressure in the vessel. 0 0