SU1388427A1 - Apparatus for measuring heat production of microorgganisms - Google Patents

Abstract

The invention relates to thermometry and can be used in determining the heat production of microorganisms in the process of growing them in a fermenter. The purpose of the invention is to improve the accuracy - due to the fact that the device is equipped with a thermostatic tank 10 filled with thermostatic liquid 9, the latter are placed in series with heat meters 5 and 6, forming the cup 8, which is mounted on the shaft 4 of the agitator 2, and The lateral surface of the heat meter 5 has a spiral-shaped groove, and on the bottom of the glass 8 a transverse groove and the shaft 4 of the agitator 2 are designed as a thermal bridge. 1 il.

Description

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The invention relates to heat metering and can be applied when determining the heat production of microorganisms in the process of growing them in a fermenter.

The purpose of the invention is to improve the accuracy of the measurement of the heat production of microorganisms during their cultivation in the fermenter.

The drawing shows devices for measuring the heat production of microorganisms.

A device for measuring heat production consists of a fermenter 1 with a stirrer 2, which are enclosed in a dry air thermostat 3. Equal-sensitizer is installed on the shaft 4, which acts as a thermal bridge

heat meters 5 and 6 are electrically connected in series with each other and the secondary device 7 and forming the cup 8.

The outer side surface of the glass 8 is provided with a spiral-forming groove, and its bottom is provided with a transverse groove. The outer surface of the cup 8 is bathed in a thermostatic liquid 9 poured into a thermostatic container 10,

In order to provide isothermal conditions for cultivation due to the intensive heat removal of the heat production of microorganisms, the shaft and the stirrer are made of a highly heat-conducting material that does not act oppressively to penetrating microorganisms. covered with a film of a material that does not inhibit the development of microorganisms.

The rotation of the agitator is carried out from the electric motor 11 of the belt drive 12 through a pulley 13 mounted on a heat-insulated shaft end 4, which is mounted on bearings 14 and 15.

The device works as follows.

Before starting the measurements, the fermenter is filled with the nutrient mixture, culture conditions are created and the steady state determines the amount of heat needed to be brought through the agitator to the nutrient medium in order to maintain

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these temperature conditions of cultivation, since, in the presence of aeration, the amount of heat carried away by air and water vapor exceeds the amount of heat released by dissipative forces .. For greater accuracy of measurement and efficiency, the amount of heat removed from the fermenter can be compensated by an additional heater installed in the fermenter. Then, the inoculum is sown,

In the process of cultivation, when heat production is released, the last over the shaft 4 of the agitator 2, which acts as a thermal bridge, and then through the heat meters 5 and 6 is diverted to the temperature-controlled fluid, Thermal EMF in the heat meters 5 and 6 is fixed by the secondary device: 7, the most continuously fix the amount of specific heat generated during the growth of microorganisms,

Heat flow sensors with a sensitivity of 1.01 W / (mV) were used as heat meters. The presence on the surfaces of heat meters 5 and 6 of the spiral-forming and transverse grooves makes it possible to reduce the area of the washed surface of the heat meters by increasing the heat transfer coefficient from heat meters to the washing liquid, as the laminar flow near the heat meters is destroyed. The reduction in the area of heat meters 5 and 6 leads to an increase in the measurement accuracy, since

with the same heat removal from the volume of cul- the metal mixture of the shaft is much lower. The presence of grooves increases heat transfer by more than 30 times.

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Thus, a device for measuring the heat production of microorganisms in the fermenter allows increasing the accuracy of measuring the heat of their viability in processes proceeding with intense heat release by 35 percent or more by stabilizing the desired cultivation temperature provided by the best heat sink of heat production from the volume of the fermenter through the agitator shaft and heat meters to a thermostated fluid washing a heat meter.

3.1388427

Claims (1)

The invention is made in the form of a glass. A device for measuring thermocoupler is formed electrically. microorganism dukdia, which are interconnected thermo-sensitizer with a stirrer and heat meter, electrical elements and installed trichetically bound to the secondary shaft on the agitator shaft, on the outer side The boron, different from that, the surface of the heat meter is made with the purpose of increasing the accuracy, it has an oral groove, and on its supply there is a thermostatic capacity, the transverse groove and shaft of the agitator are installed in the latter and the JQki is designed as a thermal bridge.