SU1386189A1 - Method of determining atretic changes in oocytes from cavitary follicles of ovary of horned cattle - Google Patents
Method of determining atretic changes in oocytes from cavitary follicles of ovary of horned cattle Download PDFInfo
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- SU1386189A1 SU1386189A1 SU864116687A SU4116687A SU1386189A1 SU 1386189 A1 SU1386189 A1 SU 1386189A1 SU 864116687 A SU864116687 A SU 864116687A SU 4116687 A SU4116687 A SU 4116687A SU 1386189 A1 SU1386189 A1 SU 1386189A1
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Abstract
Изобретение относитс к области биологии размножени и может использоватьс при изучении процесса созревани женских половых клеток, а также при разработке методов культивировани и оплодотворени вне организма ооцитов из полостных фолликулов ичников с целью получени эмбрионов ранних стадий. Целью изобретени вл етс ускорение и упрощение способа при сохранении ооцитов жизнеспособными . После вьщелени ооцита из фолликула отдел ют часть окружающего его йценосного бугорка, обрабатывают ее 0,8%-ным гипотоническим раствором цитрата натри , затем помещают на предметное стекло, диспергируют, нанос т на клетки каплю 1%-ного ацето- орсеина, накрывают покровным стеклом и исследуют под микроскопом. Наличие липидных гранул в цитоплазме клеток йценосного бугорка свидетельствует об атретических изменени х в фолликуле и ооците. с ю елThe invention relates to the field of reproduction biology and can be used in studying the maturation process of female germ cells, as well as in the development of methods for cultivating and fertilizing oocytes from the ovarian follicles of the ovary outside the body in order to obtain early-stage embryos. The aim of the invention is to accelerate and simplify the method while keeping oocytes viable. After separating the oocyte, a part of the surrounding tubercle is separated from the follicle, treated with 0.8% hypotonic sodium citrate solution, then placed on a glass slide, dispersed, a drop of 1% acetorecein applied to the cells, covered with a cover glass and examined under a microscope. The presence of lipid granules in the cytoplasm of the cells of the malignant tubercle indicates atretic changes in the follicle and oocyte. with you
Description
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Изобретение относитс к биологии размножени и может использоватьс при изучении оогенеза, а также при разработке методов культивировани и оплодотворени in vitro ооцитов из п6лостнь1Х фолликулов ичника с целью получени эмбрионов ранних стадий .The invention relates to reproduction biology and can be used in the study of oogenesis, as well as in the development of in vitro cultivation and fertilization methods for oocytes from the ovary follicles of the ovary to obtain early-stage embryos.
Целью изобретени вл етс ускорение и упрощение способа при сохранении ооцитов жизнеспособными.The aim of the invention is to accelerate and simplify the method while keeping oocytes viable.
Пример. Проводились опыты по определению атретических изменений в ооцитах из полостных фолликулов ичников коров чернопестрой породы . . Example. Experiments were carried out to determine atretic changes in oocytes from abdominal follicles of the ovary of a black-mongrel cow. .
Ооциты, окруженные клетками йценосного бугорка, вьщел ют из полостных фолликулов ичника путем разрезани их стенок. Затем под микро- скопом от каждого ооцита при помощи двух заточенных препаровальных игл отдел ют фрагмент йценосного бугорка , помещают его на 3 мин в 0,8%-ный гипотонический раствор цитрата натри , затем перенос т с минимсшьным количеством этого раствора на предметное стекло и диспергируют препаровальной иглой. Затем на клетки нанос т каплю 1% ацетоорсеина, накрывают покровным стеклом и через 1 мин исследуют под микроскопом при увеличении 20x10 и 40x10. Кратковременна обработка фрагмента йценосного бу- горка гипотоническим раствором привоOocytes surrounded by cells of the tubercle of the mesentate are extracted from the cavity follicles of the ovary by cutting their walls. Then, under a microscope from each oocyte, using a two sharpened dissecting needles, a fragment of the tubercle of the tubercle is separated, placed for 3 minutes in a 0.8% hypotonic solution of sodium citrate, then transferred with a minimal amount of this solution onto a glass slide and dispersed dissecting needle. Then a drop of 1% acetoorsein is applied to the cells, covered with a cover glass and after 1 minute they are examined under a microscope at a magnification of 20x10 and 40x10. Short-term treatment of a fragment of the yachi beech with a hypotonic solution of
дит к набуханию клеток и ослаблению межклеточных контактов, что позвол ет диспергировать клетки на предметном стекле. В клетках наблюдают наличие или отсутствие в их цитоплазме липид- ных гранул, которые вл ютс существенным признаком атретических изменений в фолликулах.This leads to swelling of the cells and weakening of the intercellular contacts, which makes it possible to disperse the cells on the slide. The cells observe the presence or absence of lipid granules in their cytoplasm, which are a significant sign of atretic changes in the follicles.
Предлагаемый способ позвол ет оценивать наличие или отсутствие атретических изменений по этому признаку и, таким образом, судить о функциональном состо нии ооцита, а также проводить отбор по этому признаку ооцитов дл культивировани и оплодотворени вне организма.The proposed method makes it possible to assess the presence or absence of atretic changes according to this trait and, thus, to judge the functional state of the oocyte, as well as to select oocytes for this condition for cultivation and fertilization outside the body.
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Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU864116687A SU1386189A1 (en) | 1986-09-10 | 1986-09-10 | Method of determining atretic changes in oocytes from cavitary follicles of ovary of horned cattle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU864116687A SU1386189A1 (en) | 1986-09-10 | 1986-09-10 | Method of determining atretic changes in oocytes from cavitary follicles of ovary of horned cattle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU1386189A1 true SU1386189A1 (en) | 1988-04-07 |
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ID=21256258
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU864116687A SU1386189A1 (en) | 1986-09-10 | 1986-09-10 | Method of determining atretic changes in oocytes from cavitary follicles of ovary of horned cattle |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU1386189A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2463977C2 (en) * | 2007-09-28 | 2012-10-20 | Витролайф Свидн Аб | Needle for taking samples |
-
1986
- 1986-09-10 SU SU864116687A patent/SU1386189A1/en active
Non-Patent Citations (1)
| Title |
|---|
| Gondos Bernard, Ultrastructure , of follicular atresia in the rat.- Gamete Research, 1982, 5,p. 199-206. Marion G. В., Gier H. Т., Chouda- ry S. B. Micromorphology of the bovine ovarian folliculer system, - Journal of Animal Science, 1968, V. 27, p. 451-471. * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2463977C2 (en) * | 2007-09-28 | 2012-10-20 | Витролайф Свидн Аб | Needle for taking samples |
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