SU1370500A1 - Device for taking samples from electrophoretic column - Google Patents

Abstract

The invention relates to laboratory technology, more specifically to devices for electrophoretic separation and the production of high molecular weight biological substances, and allows for increasing the accuracy of sampling in microgravity seizures by extracting a larger number of fractions. The device contains a hermetically separating rectangular chamber, the upper wall of which is made of polyethylene terephthalate film, and electrode chambers with electrodes separated from the separating chamber by semipermeable membranes. A fractional selection device is installed above the separation chamber, which serves to extract the resulting fractions from the separation chamber. electrophoretic separation of the object under study, by piercing the upper wall of the separation chamber with needles, is located E along the separation chamber in two rows, in one of which are within the needle-intakes fractions, and in the other - drainage needles installed in the cassette. 2 Il. (L

Description

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The invention relates to laboratory technology, more specifically to devices for electrophoretic separation and the production of high molecular weight biological substances.

The purpose of the invention is to increase the accuracy of sampling under microgravity conditions by extracting a larger number of fracdia.

1 shows a device for electrophoresis under microgravity conditions, a general view; figure 2 - the holder with needles and drive.

The device contains a sealed separating chamber 1 of rectangular cross section, the cover 2 of which is made of self-sealing material, electrode chambers 3 with electrodes 4 connected through semipermeable membranes 5, holder 6 with drainage

needles 7 with holes 8 and intake needles 9 connected through channel 10, fitting 11 and flexible pipe 12 to the pump 13. A holder 6 is mounted above the separation chamber in the brackets 14 by means of chassis screws 15 fixedly connected to the holder 6 and installed in the brackets with the help of 1 nuts 16. The brackets are attached to the housing of the separation chamber with screws 17 and straps 18 mounted on the housing of the separation chamber.

The device works as follows.

After the separation chamber 1 is filled with a buffer solution and a mixture of biological test substances is inserted into it, a constant voltage source (not shown) connected to electrodes 4 is inserted. Under the action of an electric field, the mixture of biological test substances begins to divide into zones, each of which particles with the same electrophoretic mobility located along the entire length of the separation chamber.

After the electrophoresis process is completed and the power supply is turned off by rotating the nuts 16 counterclockwise, the screws 15 transfer the forward movement of the holder 6 to the lid 2 of the separation chamber 1, resulting in the needles 1 and 9 piercing 2 and inserting it

These 8 drainage needles 7 are not inserted into the separation chamber. Then, a hand pump 13 takes samples into the intake needles 9, and due to the parallel pairwise arrangement of the drainage and intake needles and their identical diameter, each sample intake needle 9 is taken from the area equal to the width of the needle diameter, and mixing of the samples does not occur. .

Then, clockwise turning the nuts 16, file holder 6 with needles 7 and 9 above dividing chamber 1, unscrew screws 17, remove brackets 14 from separation chamber 1, and rotating holder 16 counterclockwise detach holder 6 from brackets 14.

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Thus, in each intake needle 9 there is a sample taken from a certain zone of the separation chamber 1, which can be examined after removing it from the needle.

The proposed device for sampling allows the selection of a large number of 1x as a result of electrophoretic separation of multicomponent bioobjects under microgravity conditions from virtually any zone of the separation chamber, eliminating the possibility of mixing.

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Claims (1)

Invention Formula A device for sampling from an electrophoretic column in a microgravity condition, containing electrode chambers, a separating chamber with a zipper and a sampling device, characterized in that, in order to increase the sampling accuracy 45 samples under the conditions of microgravity due to the extraction of a larger number of fractions, the sampler is designed as a holder with two rows of selected and drainage needles with the same diameter of 50 RP placed parallel to each other, while the sampling needles are equipped with a flow rate stimulator, and the drainage needles are made with top side opening, separation chamber cover 55 is sealed from a self-sealing material, and the holder is mounted above the cover with the possibility of vertical movement. (pk-.2