SU1368702A1 - Method of differential diagnostics of chronic hepatitis among children - Google Patents

Abstract

The invention relates to medicine, more specifically to pediatrics. The purpose of the invention is to increase the accuracy of the method. To do this, surface anterior skin surface scarification of the forearm in the upper third is performed in order to determine the RNA content in the macrophage cores. Scarifier remove the layer of the epidermis to the visibility of the capillary loops. The scarified areas are covered with sterile glasses. The severity of liver damage is assessed by the content of RNA in the cell preparations. S

Description

This invention relates to the field of medicine, namely to pediatrics.

The aim of the invention is to improve the accuracy of the method.

Example 1. Child P., 8 years old In the two years she suffered viral hepatitis B. After being discharged from the hospital, complaints of nucleic acid dyspepsa remained for a long time, the drugs were transferred to ethanol for 5-6 minutes. The fixed preparations are brought to water through a series of alcohols of decreasing concentration (96, 75, 50, 25%), after which they are placed in 0.2 M acetate buffer pH 4.2 for 2–3 minutes. Subsequently, the coloring preparations

tic, astheno-vegetative xa, are in a working solution of acridine

Ractera, hyperfermentemi. G 5 years diagnosed with chronic hepatitis. On admission, a state of average severity was noted; pallor, venousness, loss of appetite were observed on the skin manifestations of hemorrhagic syndrome in the form of ecchymosis, telangiectasis and erythema palmar, hepatomegaly (1.5–2 cm below the rib edge).

Liver dense consistency. According to another, a drug was not detected (the imprint of exudate with macroorgan organs was not detected. In the biochemical blood test there was a slight increase in thymol sample - up to 11.4 units (at a rate of 4 units), against the background of normal bilirubin and enzymes. In general analyzes blood and urine without pathology. In the blood, HBS, Ag was detected. In immunological status, a decrease in T-lymphocyte indices was revealed: T-act up to 13% (normal 20.8%) T-total, up to 34% (normal 69.8%), an increase in the level of immunoglobulins Ip, G to 3000 mg% (at a rate of 1230 mg%), in a sample of the skin window, a decrease in chemotaxis akrofagov to 32% (at a rate of 75%),

 For the purpose of determining the content of RNA in Drach. Macrophages, 25 were performed.

phageal cells from the second scarified skin area) are incubated in RNAase solution for 1.5 hours at 37 ° C, after which they are

orange acridine staining. The stained preparations are microscopically examined under a fluorescent microscope and fluorometrically. The RNA content in this example is 0.20 units. Chronic passive hepatitis is diagnosed.

Example 2. Child T. 4 years 11 months, suffered a severe form of viral hepatitis, after which the pathological symptoms persisted.

35 Upon admission of a child of the body of average body, are expressed: asthenia, loss of appetite, the liver is dense and protrudes from under the costal edge on

4–5 s / 3, the spleen is palpated near the surface scarification of the skin of the anterior 40 costal margin, is heard by the surface of the forearm in the upper lymph noise. HBSAg, a third of a pre-treated g biochemical analysis of the blood, was detected with blood with ethyl alcohol. A scarifier with a ,, d 29 units, (norm before 5 units) smears a layer of the epidermis in two areas of fructose-1-phosphate aldolase up (size 0.5x0.5 cm) until visibility 45 8 units. (norm up to 3 units). Reduction of capillary loops (damage to the capillary-prothrombin index to 64%; In immunological status, reduction of T-size of lymphocytes to 43% (normal 69.8%), chemotaxis of macrophages to 35% (normal

l moat and bleeding are not allowed). Scarified areas are covered with two sterile glasses, which

secure with adhesive tape. After 24 h 50 75%). The content of macrophage RNA asepot the onset of the reaction of the glass is removed. After fixation with ethanol, one of the prints is treated with RNAase, and both prints are fixed in a mixture of absolute ethanol and glacial acetic acid in a 3: 1 ratio for 11-12 minutes. Then, in order to remove traces of acetic acid and prevent acid hydrolio orange 1: 10,000 in a 0.2 M solution of acetate buffer pH 4.2. The dyeing time was 2 min, followed by washing in acetate buffer pH 4, .2 and differentiation in acridine orange solution with absolute alcohol 1: 100,000 for 20 s. After treatment with RNAase, the RNA is removed from the nucleus. Pre-fixed

25

phageal cells from the second scarified skin area) are incubated in RNAase solution for 1.5 hours at 37 ° C, after which they are

orange acridine staining. The stained preparations are microscopically examined under a fluorescent microscope and fluoromerized. The RNA content in this example is 0.20 units. Chronic passive hepatitis is diagnosed.

Example 2. Child T. 4 years 11 months, suffered a severe form of viral hepatitis, after which the pathological symptoms persisted.

35 Upon admission of a child of the body of average body, are expressed: asthenia, loss of appetite, the liver is dense and protrudes from under the costal edge on

55

tic inflammatory reaction of 0.15 units. Diagnosis: chronic aggressive hepatitis high degree of activity. . .

Clinical assessment of the current pathological process in a known manner is poorly symptomatic in a number of cases (as with persistent chronic disease). 1

Hepatitis, as well as with active chronic hepatitis), is also subjective from the point of view of a physician in the assessment of symptoms. Biochemical methods for assessing the current pathological process do not provide, in comparison with the proposed method, a sufficient prognosis duration, since only a weakly pronounced cytolysis syndrome is recorded during a period of high activity of the pathological process directly in the liver. Moreover, the indicators of biochemical tests can be normal even with an actively ongoing process in the liver in patients with chronic hepatitis.

The proposed method allows for the content of RNA in preparations of glue

 current rate of severity.

defeat the invention formula

A method for the differential diagnosis of chronic hepatitis in children by examining cells, characterized in that

increase the accuracy of the method, using a contact method, obtain a preparation of skin macrophage cells, stained with acridine orange for RNA, the content of which determines the activity

the process and the content of RNA 0,19 +

0.03 units chronic passive hepatitis is diagnosed, and when the content of RNA is 0.15iJlO, 01 units diagnose chronic active hepatitis.

Claims (1)

-F claims □ A method for differential diagnosis of chronic hepatitis in children by examining cells, characterized in that, in order to increase the accuracy of the method, a skin macrophage cell preparation is obtained by the contact method, stained with acridine orange on RNA, the content of which determines the activity of the 15 process with an RNA content of 0.19 + 0.03 units chronic passive hepatitis is diagnosed, and with an RNA content of 0.15 ^ 0.01 units, chronic active hepatitis is diagnosed.