SU1366923A1 - Spectrofluorimeter - Google Patents

Abstract

The invention relates to the field of spectral instrumentation and can be used in instruments for luminescent (fluorescent) spectral analysis. The goal is to expand the range of analyzed objects by expanding the working spectral range of the spectrofluorimeter by combining and subsequent division of optical paths. separation of the wavelengths of the emission of excitation and fluorescence on the common for two paths chamber-collimator objective. This allows the use of a spectrofluorometer to assess the quality of different, fibrous materials. 2 Il.

Description

The invention relates to spectral instrumentation and can be used in instruments for luminescent (fluorescent) spectral analysis.

The aim of the invention is to expand the functionality of the spectrofluorometer by expanding the working spectral range.

FIG. 1 shows the principal optical layout of the spectrofluorimeter; in fig. 2 shows the characteristics of two types of spectral separation coatings that can be applied to the outer surface of the chamber-collimator lens.

The spectrofluorometer contains optically coupled source 1. of the excitation path, focusing system 2 of the excitation path, entrance slit 3, collimator lens 4 of the excitation path, diffraction grating 5 of the excitation path, rotary mirror 6 of the excitation path, chamber-shaft 25 excitation radiation wavelength lens 7, which for the luminescent surface of the excitation non-path is a chamber, transparent object fixed in a fluorescence path for a collision of stimulating radiation through a focusing lens Thread 2, an entrance slit 3, and to limatorny lens 4 is directed onto the diffraction grating 5 at an angle of incidence of the definitions where diffracted

20 Ruets. The diffracted radiation of the rotating mirror 6 is directed into the lens objective7 and is focused in the form of a spectrum. The output slit 8 causes a certain spectral inter

the holder 9. The irradiated part of this surface emits fluorescence

the spectral slit 8, which is the output for the excitation path and the input for the fluorescence path, the holder 9 of an opaque object with a luminescent surface (a variant of the object in the immediate vicinity of the spectral slit 8 is shown), a reflective element 10 fluorescence tract , a fluorescence path diffraction grating 11, a fluorescence chamber 12 lens, a fluorescence path exit slot 13 and a fluorescence path detector 14.

FIG. 2 shows the dependence of the reflection coefficient on the wavelength for two types of coatings: the C15-29I-24I coating, fifteen-fold, obtained by evaporation in vacuum of zinc sulfide and magnesium fluoride — the coating C23-57IE-41IE, twenty-four layers, obtained by evaporation in vacuum zirconia and silica

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The use of a particular type of coating depends on the problem being solved with the help of the device. The reflecting element introduced into the optical path of the separation of the wavelength and radiation of fluorescence is made in the form of a spherical concave mirror centered with respect to the objective 7. Radius

excitation radiation wavelength shaft onto the luminescent surface of a non-opaque object hardened in

The curvature of the reflective element 10 is so coordinated with the radius of curvature of the outer surface of the lens 7 and with its focal distance that a centered optical system consisting of two mirrors is formed, similar to the fluorescence path of a Cassegrain lens. The focus of this system is slot 8.

Spectrofluorometer works as follows.

The radiation from the source 1 in the optical path of the selection of the frequency of the exciting radiation through the focusing system 2, the entrance slit 3 and the collimator lens 4 is directed to the diffraction grating 5 at a certain angle of incidence, where it is diffracted. The diffracted radiation of the rotating mirror 6 is directed into the lens objective7 and is focused in the form of a spectrum. The output slit 8 imposes a certain spectral range of wavelengths of the excitation radiation on the luminescent surface of an untransparent object hardened in

the holder 9. The irradiated part of this surface emits fluorescence

to slit 8, which for the optical pathway of fluorescence wavelength is input. Fluorescence emission from the reflective outer surface of the lens objective 7

and a reflective element — a spherical concave mirror 10 — is directed in the form of a parallel beam to the diffraction grating 11. The diffracted radiation is focused by a camera: a spectral objective 12 in the form of a spectrum; 14.

Spectrum scanning by wavelengths of both excitation and fluorescence radiation is performed by rotating diffraction gratings 5 and 11 using stepper motors (not shown).

The invention allows the spectrum to be scanned by wavelength independently in each optical path, which ensures the use of

spectrofluorometer to analyze a wide range of materials for which the required wavelengths of the excitation radiation must be selected from a wide spectral range

In addition, the invention allows simultaneous scanning of wavelengths in both paths, which makes it possible to analyze complex multicomponent substances.

Claims (1)

The invention includes a spectrofluorometer comprising an optically coupled optical path for selecting a frequency of excitation radiation and an optical path for selecting a frequency for fluorescence emission, which include the common path for two paths. a camera-collimator lens and a spectral slit, characterized in that, in order to expand the range of objects being analyzed by expanding the working spectral range, a reflective element optically coupled to the spectral slit is introduced into the optical path of fluorescence emission frequency selection - Matrix lens, made with the possibility of selective spectral reflection of fluorescence radiation. te / % % .N S Sh “N §1 §