SU1345115A1 - Method of determining aggregation capacity of thrombocytes - Google Patents

Abstract

The invention is intended to investigate the platelet component of hemostasis. The purpose of the invention is to improve the accuracy and sensitivity of the device. The device for mixing blood plasma consists of a bed 1, a bearing 2, a tube 3, a cell 4 and a driven pulley 5 The photoelectrocolorimeter cell is filled 90% of platelet-rich blood plasma, the aggregating agent J is added and closed. provide rotation in a plane perpendicular to the passage of a beam of light at a speed of 40-60 rpm. The air bubble moving along the cuvette provides atraumatic mixing of platelets, and the rotation of the cuvette excludes the sedimentation of platelets and their aggregates, 1 dl., 3 table. OO 4 followed

Description

1134

The invention relates to medicine, specifically to clinical angiology and coagulogy, and can be used to study the platelet component of hemostasis.

The purpose of the invention is improving the accuracy and sensitivity of the method "

The goal is achieved by providing rotation of 90% filled blood plasma and a closed cuvette at a speed of 40-60 rev / min relative to the axis of the light beam. Puzzéek of air remaining in the cuvette, moving along the perimeter of the cuvette, provides for the platelet mixing which is necessary for ensuring contact these cells and their interactions between themselves, and the rotation of the cuvette excludes the sedimentation of platelets and their aggregates (plasma stabilization)

The drawing shows a diagram of an apparatus for carrying out the method.

A device for mixing blood plasma enriched with platelets contains a bed 1, a bearing 2, a tube 3, a cuvette holder 4, a driven pulley 5, connected by a driving pulley on an electric motor shaft with a variable number of revolutions

The method is carried out as follows.

A FEK-56 photoelectric calorimeter cuvette with a volume of 1.7 megapixels and with a working layer thickness of 3 mm is filled with 90% of platelet-rich (250 x X 10 Vl - 300 X lOVn) test plasma, add ADP, close and provide rotation in the joints perpendicular to the passage of the light beam relative to the axis of the latter at a speed of 40-60 rpm. The air bubble remaining in the cuyette (10% of the cell volume), moving along the perimeter of the latter, provides atraumatic mixing of platelets, and rotation of the cuvette excludes sedimentation of platelets and their aggregates about

As the platelets aggregate and disaggregate, the optic density changes, as recorded by the EPP-09-MZ recording electronic potentiometer.

The following parameters are distinguished on the aggregatograms: T ,, is the time of the start of aggregation (from the moment of addition of the inducer of aggregation (adenosine diphosphate) to the plasma before the start of aggregation), c-Tj, is the time of aggregation (from the moment of addition of adenosine diphosphate to the point of minimum optical density of the plasma) 5 s; T, is the rate of aggregation (the time over which the optical density of the plasma decreases by 50%), s;

: four

- maximum aggregation time

(the time for which the optical density of the plasma does not change from the maximum aggregation until the beginning of the disaggregation), s; T5, - disaggregation rate (the time over which the optical density of the plasma increases by 50%), Co

TabLo and 2 present data confirming the achievement of a positive effect from the use of the proposed method in comparison with the known method.

The tabLOZ provides data on the reproducibility of the method using the rotation speed of the cuvette 40. and 60 rpm about

Claims (1)

Invention Formula The method of determining the aggregation capacity of platelets, including the preparation of enriched thrombocytes. container plasma, adding an aggregating agent, mixing the photometer in the cuvette and registering the optical density of the mixture, characterized in that, in order to improve the accuracy and sensitivity of the method, the movement is carried out by rotating the closed cell at a speed of 40-60 rpm, while filling the cuvette the analyzed mixture is not less than 90%. te h o h n ..vO -C-4 c - " CMG - CM “-Sch - I-, .m " COOP - With f P r.-cm-- CN CM .1345115 Table 3 Specific examples of the implementation of the proposed method for determining platelet aggregation Ti 20 400 Editor L.Povkhan Compiled by ArAgureyev Tehred M. DIAYAK 4913/43 Circulation 775 Subscription VNIRSHI State Committee of the USSR for inventions and discoveries 113035, Moscow, Zh-35, Raushsk nab., d.A / 5 Production and printing company, Uzhgorod, Projecto st., 4 20 20 20 405 810 800 Corrector S.Cherni