SU1320223A1 - Device for identification of microorganisms - Google Patents

Abstract

The invention relates to medical technology and is intended for microbiology. The purpose of the invention is to stabilize moisture during the cultivation of microorganisms. The device consists of a container 1 in which a working panel 2 is installed. In the working panel 2 there are cells 4 The bottom of the cells is made in the form of cones. In the container 1 there are longitudinal grooves. Vertices of cones are located along the axis of the grooves. In the cells 4, the wall of the container 1 is made stepwise, which leads to the formation of a gap between the lid 3 and the working panel 2, the device is made of a polymeric material. 3 il. SO GO o y to with FT8.1

Description

11320223

This invention relates to microbiology and is intended for the identification of microorganisms.

The aim of the invention is to stabilize the moisture during cultivation of microorganisms.

Fig, 1 shows the device, a general view; in fig. 2 - working panel; in fig; 3 - base.

The device consists of container 1, in which working panel 2 is installed, and screws 3. In working panel 2 there are cells 4 for differential Differential diagnostic diagnostics solutions, they are prepared on the basis of phosphate buffers. A differentiating substrate is used as the main component; the change in pH is detected using an indicator. For example, urea is used as a substrate for detecting urease, bromocresol red is used as an indicator. A suitable hydrogen sulfide scavenger is sodium thiosulfate in the presence of an iron salt. For the detection of lysine decarboxylase, ornithine, and dihydrolase arginine diagnostic media. The bottom of the cells is made in the form of cones 5. The container uses lysine, ornithine, and arginer 1 has longitudinal grooves nick and bromthymol blue indicator 6, along which vertices are located. To determine the fermentation of the carbon cone 5, to maintain optimum the humidity in the cells 4 of the wall 7 of the container 1 is made of stepwise, sorbitol) is used appropriately, which leads to the formation of the zazy substrates in the presence of indicar between the lid 5 and the working patch of phenol red. Substrate 2

The device is made from polides (glucose, lactose, mannitol, sucrose, arabinose, maltose, inosi30

Compounds for the determination of deaminases are amino acids such as phenyl-alumina material by means of a vacuum formulator and tryptophan, while chromogenic material is quenched. For the manufacture of the carrier, the sub-lactosidase substrate is; a stratum (working panels 2 with cells | 4 and base) use a rigid white PVC lighting film of group II, S 0.55 mm. For the manufacture of the cover 3, a transparent vinyl calender calender is used: a film of the type CPS, S 0.5-0.7 mm. Substrates are applied to the centers of the cells 4 as a point with 35 pipettes, 0.02 mm each, into one of the parts of the pipette dispenser P1-0.02. Pa- ® 4 of the working panel 2 “With the same barrel panel 2 with cells 4, they are placed in the base of the container and closed with the cover 3, after which the device is packed tightly in polyethylene. - The device is used as follows

Components of the device are washed and sterilized with ultraviolet light. The cells 4 of the working part of the panel 2 are filled into the container in 45 containers, closed with various differential diagnostics 3, sealed in polyethylene and stored in liquid form, and then dried. The number of cells 4 corresponds to the number of tests ensuring the complete differentiation of bacterium from the agar medium. Use the terium of the intestinal family. , CL difely fresh cultures (18-24 h), renziopschi suggests using cultures older than 30 h. The following tests can give false samples: sodium citrate, few negative results. rub, sodium citrate with glucose. Suspension of microorganisms should lysine, arginine, ornithine, phenylalanine, j have exactly visible turbidity and equal.

0-nitrophenyl-p-galactopyranoid, etc.

Example A reagent mixture is prepared consisting of 120 g of carbohydrate and 0.85 g of phenol red, which is dissolved by heating in 1 liter of phos-. Fat buffer pH 8.0. The prepared solution is excavated with a dispenser.

way digging and other solutions of differential-diagnostic media. So, all 20 cells 4 of the working panel 2 are filled. Then the panels 2 are dried in a drying cabinet for 4 hours with further sterilization with ultraviolet for 1 hour. Then the panel 2 with cells 4

Then, as needed, they are used to identify microorganisms. For this purpose, a suspension of microorganisms is prepared.

indole, acetylmethylcarboiyl, urease, hydrogen sulfide, glucose, / 1-galactoside, lactose, mannitol, sucrose, icoit, sorbitol, arabinose, maltose.

at least a turbidity standard of -500 ppm / ml microbial cells for freshly isolated cultures. Lower cultures can be found in museum cultures.

Differential-diagnostic media solutions are prepared on the basis of phosphate buffers. A differentiating substrate is used as the main component; the change in pH is detected using an indicator. For example, urea is used as a substrate for detecting urease, bromocresol red is used as an indicator. A suitable hydrogen sulfide scavenger is sodium thiosulfate in the presence of an iron salt. To detect lysine, ornithine decarboxylase, and arginine dihydrolase, lysine, ornithine, arginine, and bromothymol blue indicator are used. To determine the fermentation of carbon, sorbitol, appropriate substrates are used in the presence of a phenol red indicator. Substrate-

 lysine, ornithine, arginic and brominated blue indicator are used. To determine the fermentation of carbon, sorbitol), appropriate substrates are used in the presence of a indicator of phenol red. Substrate-

Dov (glucose, lactose, mannitol, sucrose, arabinose, maltose, inosi30

5 nick and tryptophan, and the chromogenic-galactosidase substrate is 35 pipettes, 0.02 mm each in one of the 4 working panels 2 "Similar

0-nitrophenyl-p-galactopyranoid, etc.

Example A reagent mixture was prepared consisting of 120 g of carbohydrate and 0.85 g of phenol red, which was dissolved by heating in 1 liter of phosphate buffer pH 8.0. The prepared solution is excavated with a dispenser.

5 nick and tryptophan, and the chromogenic-galactosidase substrate is 35 pipettes, 0.02 mm each in one of the 4 working panels 2 "Similar

45 Thiomineal in a container, close the lid 3, sealed in polyethylene and the store of ananism from an agar medium. Only fresh cultures (18-24 hours) are used. Cultures older than 30 hours can give false-negative results. Microbial suspension must have exactly visible turbidity and is equal to

way digging and other solutions of differential-diagnostic media. So, all 20 cells 4 of the working panel 2 are filled. Then the panels 2 are dried in a drying cabinet for 4 hours with further sterilization with ultraviolet for 1 hour. Then the panel 2 with cells 4

Thickening in a container, closed with a lid 3, sealed in polyethylene and stored with agar medium. Only fresh cultures (18-24 hours) are used. Cultures older than 30 hours can give false-negative results. Suspension of microorganisms must have exactly visible turbidity and equal.

Then, as needed, they are used to identify microorganisms. For this purpose, a suspension of microorganisms is prepared.

at least a turbidity standard of -500 ppm / ml microbial cells for freshly isolated cultures. Lower cultures can be found in museum cultures.

ten

f5

levels of enzymatic activity than in fresh clinical ones, therefore, a more dense inoculum is prepared, approximately equal to the turbidity standard of 1 billion / ml of microbial cells. The suspension is prepared in 4 ml of sterile saline solution pH 6.0 + 0.5,

Inoculation and incubation.

Open the packaging. Record sample number and other required information. Open the lid 3 of the container and place it on the table. Take out the working panel 2 and pour water into the grooves 6 of the base of container 1. Insert panel 2 with cells 4 into the base of container 1, Pipette 0.15 ml of the microorganism suspension into each reaction cell 4, except for the hydrogen sulfide test, 2Q where just one drop of the suspension (0.05 ml). Pour over. cell 4 with hydrogen sulfide dough 0.1 ml of melted and sufficiently cooled (to) semi-liquid agar. In order to create anaerobic conditions, anaerobic conditions are covered with sterile vaseline oil over the test cells; lysine, arginine, ornithine, and hydrogen sulfide (0.1 ml - 2-3 h), close the lid 3 of the container. They are incubated for 18-24 h at t. After the end of the incubation, the lid 3 of the container is opened and 1 porridge of 10% FeClj is added to the cell 4 with the phenylalanine deaminase test, and the rum is relative to the working panel.

Acetylmethylcarbinol test - 1 liter of 6% o-naphthol and then 1 ka of 40% KOH; 1 drop of Ehrlich reaction into a cell with dough n-indole formation,

Reading the reaction.

Read all reactions immediately positive or negative with lacno with color changes, test for acetylmethylcarbinol.

The test for methylmethyl carbinol is allowed to stain for about 10 minutes, then read the reaction.

The device allows to sufficiently accurately identify the bacteria from the intestinal intestine to the species, has a high response rate, is convenient to install, economically, always in use and does not require the consumption of bacteriological utensils.

Claims (1)

Formula invented A device for identification of microorganisms, made in the form of a tineer with a crush and a working panel with cells, characterized in that, in order to stabilize the quality during the cultivation of microorganisms, long grooves are made in the container, and the bottom of the working cells is not filled in the form of cones, tires which are located along the wasps of the grooves, and the cover is installed with , rum relative to the working panel. Acetylmethylcarbinol test - 1 drop of 6% o-naphthol and then 1 drop of 40% KOH; 1 drop of Erlich reagent into a cell with the test for indole formation, Reading the reaction. All reactions are read immediately as positive or negative according to color variations, excluding the acetylmethylcarbinol test. The acetylmethylcarbinol test is allowed to stain for about 10 minutes, then read, the reaction. The device allows you to accurately identify the bacteria of the intestinal family to the species, has a high response rate, is convenient in setting, economical, always ready for use and does not require the cost of bacteriological dishes. Invention Formula A device for identifying microorganisms, made in the form of a container with a crush and a working panel with cells, characterized in that, in order to stabilize the moisture during the cultivation of microorganisms, longitudinal grooves are made in the container, and the bottom of the working panel cells are conical, whose tops are located along the the axes of the grooves, and the cover is installed with )Yu l n A: Aloid fig 2 AND  turn fiyo.Z Editor K. Rybchenko Compiled by C „Klypkin Tehred I.Popovich Order 2612/22 Edition 499 Subscription VNIIPI USSR State Committee for inventions and discoveries 113035, Moscow, Zh-35, Raushsk nab., 4/5 Production and printing company, Uzhgorod, st. Project, 4 Proofreader L, Pilipenko