SU1319352A1 - Method for purifying h4 hystone received from thymus tissue - Google Patents
Method for purifying h4 hystone received from thymus tissueInfo
- Publication number
- SU1319352A1 SU1319352A1 SU3861169/14A SU3861169A SU1319352A1 SU 1319352 A1 SU1319352 A1 SU 1319352A1 SU 3861169/14 A SU3861169/14 A SU 3861169/14A SU 3861169 A SU3861169 A SU 3861169A SU 1319352 A1 SU1319352 A1 SU 1319352A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- protein
- column
- hydrochloric acid
- histones
- histone
- Prior art date
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
FIELD: molecular biology. SUBSTANCE: method involves solving a sample of general histone sulfate received from calf thymus in hydrochloric acid adding drop-by-drop perchloric acid to reach the end concentration of 0.5 M. Insoluble H2A, H2B, H3 and H4 histones precipitate is formed. The precipitate is separated from H1 histone soluble in perchloric acid by means of centrifuging. The sample having H2A, H2B, H3 and H4 histones is solved in a solution consisting of 0.02 N hydrochloric acid, 0.05 M sodium chloride solution with pH 1.7. P-30 Acrylex chromatography is performed at the room temperature. Protein elution from the column is carried out by means of source solution. Recording the amount of protein output from the column is made in spectrophotometric way at 230 H wave length. Protein fractions corresponding to each protein peak are united, subjected to dialysis against 0.01-0.02 N hydrochloric acid at 4 C and lyophilized. Protein output from a column is 95% EFFECT: high bactericide activity of the preparation. 1 dwg
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU3861169/14A SU1319352A1 (en) | 1985-01-07 | 1985-01-07 | Method for purifying h4 hystone received from thymus tissue |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU3861169/14A SU1319352A1 (en) | 1985-01-07 | 1985-01-07 | Method for purifying h4 hystone received from thymus tissue |
Publications (1)
Publication Number | Publication Date |
---|---|
SU1319352A1 true SU1319352A1 (en) | 1995-09-10 |
Family
ID=60519405
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SU3861169/14A SU1319352A1 (en) | 1985-01-07 | 1985-01-07 | Method for purifying h4 hystone received from thymus tissue |
Country Status (1)
Country | Link |
---|---|
SU (1) | SU1319352A1 (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100356853C (en) * | 2001-08-29 | 2007-12-26 | 瑞典环境Svv股份公司 | Antimicrobial agent |
RU2471873C2 (en) * | 2010-11-30 | 2013-01-10 | Учреждение Российской академии наук Институт биологии Уфимского научного центра РАН (ИБ УНЦ РАН) | Method of preparative recovery of basic proteins from supramolecular structures of escherichia coli growing population |
CN106232614A (en) * | 2014-05-29 | 2016-12-14 | 新加坡科技研究局 | Protein extracting method |
-
1985
- 1985-01-07 SU SU3861169/14A patent/SU1319352A1/en active
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100356853C (en) * | 2001-08-29 | 2007-12-26 | 瑞典环境Svv股份公司 | Antimicrobial agent |
RU2471873C2 (en) * | 2010-11-30 | 2013-01-10 | Учреждение Российской академии наук Институт биологии Уфимского научного центра РАН (ИБ УНЦ РАН) | Method of preparative recovery of basic proteins from supramolecular structures of escherichia coli growing population |
CN106232614A (en) * | 2014-05-29 | 2016-12-14 | 新加坡科技研究局 | Protein extracting method |
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