SU1313812A1 - Method for testing bactericides for fighting petroleum industry microflora - Google Patents

Description

 3

The invention relates to the field of fights with bacterial contamination of productive layers of oil fields and the inner surface of oil field equipment and can be used in the oil, gas, oil refining, medical and microbiological industries.

The purpose of the invention is to improve the accuracy of the test results.

Before starting the dosing of a bactericide, samples of a solid substrate are infected once with oilfield microorganisms and until the maximum possible (or other conditions given for testing the number of microorganisms fixed on the surface of the solid substrate) are enriched, the liquid medium is enriched with nutrient components necessary for accelerated development of microorganisms, then liquid the medium is replaced by the natural liquid habitat of the microorganisms. After stabilization of the number of microorganisms on the surface and substrate in a liquid |, th medium dosed bactericide after cessation of dosage which continue feeding the liquid medium to remove germicide from the surface of the solid substrate, after which the determined number visivpshh microorganisms attached to a solid substrate.

Example. A known method using as seed seed wastewater Samotlor field containing 2, kg / / cm SSC and 3.6-10 cells / ml heterotrophs growing on m septon agar (TMS), determine the minimum concentration of formaldehyde, suppress the complete microflora of wastewater at a contact time of 24 hours. This concentration is 75 mg / l,

The flow chambers -3, which are glass vessels of np - the mongole angle of 250x20x3 mm with nozzles in the tops and lower end clamps, are hung from steel samples of 20x15x0.3 mm (6 samples each in the needle). In flowing chambers 4-6 with a length of 100 mm and an internal diameter of 5 mm, 2 g of crushed core rock of the BV-8 productive layer of the Samotlor field (fraction 0.5-1.0 mm) are placed and the chambers are closed at the ends of glass swabs.2 .2

cotton wool Flow chambers are placed in a thermostat with a temperature of + 35 ° C and connected with flexible plastic tubes (inner diameter 4 mm) with

metering pumps designed for continuous pumping of fluid through the flow chambers, and with drain tanks located outside the thermostat. The entire system is sterilized by sesame filling the system with a 30% formalin solution, after which the disinfecting solution is squeezed out of the system with a 10% solution of Postgate-B nutrient medium in sterilized formation water of the Samotlor field, which is pumped with dosing pumps at a speed of 500 cm per day every camera. After removing traces from the system

Formalin, 5 cm of waste water from the Samotlor field with a content of sulfate reducing bacteria — 2.410 cells / ml is introduced into each chamber, after which the solution is continued to be pumped through the chambers.

Postgate-B d in formation water with

at the same rate for 15 days (until the microorganism content in the water at the outlet from the chamber is stabilized). Then, a 10% solution of the post-post gate medium, pumped through chambers 1 and 4, is replaced with sterilized formation water (control), and the solution pumped through chambers 2, 3, 4, and 6 with the same in formaldehyde solutions.

35 water with a concentration: chambers 2, 4 - 75 mg / l; chambers 3, 6 - 1000 mg / l.

After 24 hours, the formaldehyde solutions are replaced with sterile formation water.

40 to remove the chambers from formaldehyde, after which the pumping of water through all the flow chambers is stopped, the steel samples and rock samples from the chambers are removed and subjected to examination,

45 in order to determine the residual contents of living cells of mycoorganisms.

The research results are summarized in table. 1 and indicate that formaldehyde concentration

50 75 mg / l with a contact time of 24 hours is sufficient to suppress microorganisms that are suspended in water, and insufficient to suppress microorganisms sorbed on the surface of the formation and samples of steel coated with corrosion products.

Formaldehyde concentration in water up to 1000 mg / l is sufficient for almost complete destruction.

microorganisms sorbed on solid substrates.

Comparative results of testing the effectiveness of the use of the form-part of expensive and time-consuming

The lane for the suppression of SSC is given in the industrial tests of the Bactettable. 2. pesticides, which greatly accelerates

The rates of research on the selection and results of tests of the agreement of technologies for the use of bacteriinally preventable methods allow opioids to combat sulfate reduction

determine the dosage and duration of microbiological corrosion on

injecting bactericides sufficient for oil deposits.

Table 1

Indicators Samples of steel. Edi- Samples of formation rock

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 I-j i lL-1 1E1 1 11

Concentration

formaldehyde

mg / lO 75 1000 mg / l O 75 1000

The residual content of sulfate reducing bacteria, cells / cm 2,0-10 3,25-100,4 cells / g 2,4-10 l.l-io GR

Residual TBC, 54

cells / cm 2,6-10 9,2-10 24,0 cells / g 4,8-10 7,0-10 3,6-0

Table -2 Method Concentration of formaldehyde, mg / l

50 75 1 100 250 500 1000

Prototype

On suspension of SSC cells in water

Base

SRB in the PPP of injection wells The proposed SRB on samples of formation rocks of UHV on samples of tubular steel suppression of bacteria in field conditions. Consequently, the application of the proposed test method makes it possible to refuse to conduct pain 100

100

100

100

62.0

100

24

48

100

13

65

100

Claims (4)

1. METHOD FOR TESTING BACTERICIDES FOR THE FIGHT AGAINST OIL INDUSTRIAL MICROFLORA, comprising contact of oilfield microorganisms with a bactericide and subsequent determination of the number of surviving microorganisms, characterized in that, in order to increase the accuracy of the test results, samples of the solid substrate once infect the microorganisms to the maximum heat level the latter, fixed on the surface of a solid substrate, the liquid medium is enriched with nutrient components, after Num laziness tion of microorganisms in a liquid medium is introduced bactericide. 2. The method of pop. 1, characterized in that after the enrichment of the liquid medium with nutrient components, the liquid medium is replaced with formation or oilfield wastewater. 3. The method according to claims 1 and 2, characterized in that as a solid substrate using rock samples of oil-bearing strata and / or metal samples of oilfield equipment. 4. The method according to claims 1 to 3, about t l and characterized in that formaldehyde is used as a bactericide. □ about go 1 “131