SU1312452A1 - Tray for performing luminescent measurements - Google Patents

Abstract

This invention relates to luminescence analysis. The aim of the invention is to increase the luminescence radiation while maintaining the possibility of placing the cell directly in the cuvette holder of a serial spectro fluorometer. For this reason, all faces, except the output one, are made reflective in the cuvette. The light from the excitation source is introduced into the cuvette through the central opening perpendicular to the frontal side of the cuvette and through a prism located on the opposite side, is reflected many times from the edges of the cuvette. 2 Il. (L

Description

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The invention relates to luminescence analysis, in particular, to the technique of measuring the luminescence of solutions, and can be used to measure the intensity and lifetime of the luminescence of weakly absorbing solutions in physicochemical and biomedical research.

The purpose of the invention is to increase the luminescent radiation while maintaining the possibility of placing the cell directly in the cuvette holder of a serial spectrofluorometer,

FIG. Figures 1 and 2 show a cuvette indicating the course of the light rays.

The device consists of a cell for the sample under study, made of quartz or optical glass, on whose face outside or inside a reflective layer 2 of aluminum or silver is applied, on the front side of the layer there is a narrow slot 3 for entering the exciting light, and on the side of the cell there is a window 4 for luminescence light collection. Inside the cell there is a triangular prism 5 with reflective leg faces 6, located at the rear wall opposite the slit.

The device works as follows

The proposed cuvette is placed in the cuvette compartment of the spectrofluorometer, fluorimeter or phosphorimeter. The exciting light enters the cell 1 through the slit 3 located on the front side of the cell, is reflected from the leg faces 6 of the triangular prism 5, hits the opposite face of the cell and is reflected by the reflective layer 2 to the opposite face, etc. until completely absorbed by the subject

by a sample .. The luminescence is collected through a window 4 located on the side face of the cell due to the return of the luminescence light from the reflecting side face and due to the direct exit in the direction of registration. The reflecting side face, opposite to the secondary radiation exit window, doubles the number of reflections of the exciting light.

The use of the invention in comparison with the known devices allows for a higher luminescence intensity of the sample,

The proposed cuvette can be used in standard industrial spectrofluorometers, fluorimeters and phosphorimeters without changing their design.

Claims (1)

Invention Formula Cuvette for fluorescent measurements A scientific research institute containing a cell for the sample under study, bounded by parallel reflective surfaces, has a window in one of the surfaces for entering the excitation light beam, characterized in that A triangular prism with reflective edges is inserted into the cell, the base is mounted to the rear wall of the cell opposite the window for entering the exciting light beam, centered on the reflecting surface of the cuvette, while the surface of one of the side faces of the cuvette is made reflective. "Vv   jfijj about (ptisJ Editor G. Volkova Compiled by B.Shirokov Tehred A. Kravchuk Order 1965/41 Circulation 777 VNIIPI USSR State Committee for inventions and discoveries 113035, Moscow, Zh-35, Raushsk nab., 4/5 Production and printing company, Uzhgorod, Projecto st., 4 J / a i s .g.d Proof-reader I.Erdeyi Subscription