SU130160A1 - Method for determining the toxicity of fungi - Google Patents

Method for determining the toxicity of fungi

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Publication number
SU130160A1
SU130160A1 SU642904A SU642904A SU130160A1 SU 130160 A1 SU130160 A1 SU 130160A1 SU 642904 A SU642904 A SU 642904A SU 642904 A SU642904 A SU 642904A SU 130160 A1 SU130160 A1 SU 130160A1
Authority
SU
USSR - Soviet Union
Prior art keywords
toxicity
fungi
determining
minutes
exposed
Prior art date
Application number
SU642904A
Other languages
Russian (ru)
Inventor
И.И. Очкина
Н.А. Спесивцева
Original Assignee
И.И. Очкина
Н.А. Спесивцева
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by И.И. Очкина, Н.А. Спесивцева filed Critical И.И. Очкина
Priority to SU642904A priority Critical patent/SU130160A1/en
Application granted granted Critical
Publication of SU130160A1 publication Critical patent/SU130160A1/en

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Description

Существующие способы определени  токсичности грибов, выдел емых из кормов и натологических материалов, представл ют собой комнлекс СЛОЖНЫХ, продолжительных и дорогосто щих исследований.Existing methods for determining the toxicity of fungi released from feed and natural materials are a complex of complex, lengthy and expensive studies.

Предлагаемый способ позвол ет в течение времени от 3 мин до 2 час определить токсичность культуры гриба, благодар  использованию тестобъекта протозоа.The proposed method makes it possible to determine the toxicity of the culture of the fungus over a period of time from 3 minutes to 2 hours, thanks to the use of a protozoa test object.

Способ быстрого определени  токсичности грибов заключаетс  в следующем. Культура гриба, выделенна  обычным микологическим методом на ПЛОТНОЙ питательной среде, отдел етс  от нее и экстрагируетс  при температуре минус 4° нейтральной водопроводной водой в течение суток. Затем на предметное стекло нанос т одну каплю культуры протозоа Paramaecium caudatum или Tetrahymena pyriformis.A method for rapidly determining the toxicity of fungi is as follows. The culture of the fungus, isolated by the usual mycological method on a DILIC nutrient medium, is separated from it and extracted at a temperature of minus 4 ° with neutral tap water for a day. Then, one drop of Paramaecium caudatum or Tetrahymena pyriformis protozoan culture is applied to a glass slide.

Под воздействием экстрактов из культур грибов гибель и разрущение простейщих организмов наступает в течение не более 3 мин при воздействии резкотоксичных щтаммов, в течение не более 8 мин при воздействии токсичных щтаммов и в течение не более 1-2 час при воздействии слаботоксичных щтаммов.Under the influence of extracts from cultures of fungi, death and destruction of simple organisms occurs within no more than 3 minutes when exposed to sharply toxic substances, within no more than 8 minutes when exposed to toxic substances and for no more than 1-2 hours when exposed to weakly toxic substances.

Предмет изобретени Subject invention

Способ определени  токсичности грибов, отличающийс  тем, что, с целью ускорени  определени  токсичности грибов, поражающих корма, в качестве тест-объекта используют протозоа Paramaecium caudatum или Tetrahymena pyriformis.The method of determining the toxicity of fungi, characterized in that, in order to accelerate the determination of the toxicity of fungi that infect fodder, the protoma Paramaecium caudatum or Tetrahymena pyriformis is used as a test object.

SU642904A 1959-11-03 1959-11-03 Method for determining the toxicity of fungi SU130160A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SU642904A SU130160A1 (en) 1959-11-03 1959-11-03 Method for determining the toxicity of fungi

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SU642904A SU130160A1 (en) 1959-11-03 1959-11-03 Method for determining the toxicity of fungi

Publications (1)

Publication Number Publication Date
SU130160A1 true SU130160A1 (en) 1959-11-30

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Family Applications (1)

Application Number Title Priority Date Filing Date
SU642904A SU130160A1 (en) 1959-11-03 1959-11-03 Method for determining the toxicity of fungi

Country Status (1)

Country Link
SU (1) SU130160A1 (en)

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