SU1276668A1 - Nutrient medium for growing anaerobic spore-forming bacteria - Google Patents

Abstract

The invention relates to medicine, to microbiology, intended for the cultivation of anaerobic bacteria. The purpose of the invention is to increase the biomass yield due to the modification of the nutrient medium. For this, redox-polymer with sulfhydryl groups, a dehydration reaction of polyvinyl alcohol, oxidation of the resulting conjugated double bonds to obtain oL-hydroxy and interaction with potassium sulfhydrate are used as reducton. At the same time betaC rut the following ratio of components, g / l: D-glucose 2.5-3.0; sodium (L chloride 4.0-5.0; redox-polymer with sulfhydryl groups 2.0-3.2, mi-peptone broth - the rest. 2 tablets. o O5 O) 00

Description

one

This invention relates to medicine, in particular, to microbiology, and is intended to grow anaerobic bacteria.

The purpose of the invention is to increase the biomass yield by modifying the nutrient medium.

The invention is illustrated by the following examples.

Example 1. Redoxite with sulfhydryl properties is obtained by sequentially carrying out the dehydration reaction of polyvinyl alcohol, oxidizing the resulting conjugated double bonds to form d-hydroxy and reacting with potassium sulfhydrate.

The nutrient medium is prepared as follows.

 In the conical cones with a capacity of 50 ml, redox polymer is introduced in an amount of 2.0 g, which corresponds to the sulfur content in the polymer of 0.34 g / l. D-glucose 2.5 g / l, sodium chloride 4.0 g / l. The medium is sterilized in an autoclave at 0.5 atm., 30 min. Biomass yield 464 + 50 kdlony / in 0.1 ml, (dilution Yu).

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Example 2. In a conical cone with a capacity of 50 ml, a weight of 3.2 g of polymer was added, which corresponds to a sulfur content of 5 0,8 r / ji in the polymer, 25 ml is poured. m co-peptone broth (amine nitrogen content 120-130 mg%), D-glucose 3.0 g / l, sodium chloride 5.0 g / l, then sterilized at 0.5 atm for 30 minutes. The accumulation rate of clostridia is 536 + 11 m colonies / 0.1 ml (497 + 82 - using a known medium).

Example 3. In a conical cones with a capacity of 50 ml put a portion of the redox polymer 2.8 g, which corresponds to the sulfur content in the polymer of 0.7 g / l, pour 25 ml of m-peptone broth (amino nitrogen content 120-130 mg%), 2 , 7 g / l of glucose, 4,5 sodium chloride, then the medium is sterilized at 0.5 atm for 30 minutes. The intensity of accumulation of clostridium cells in the medium is 709 ± +36 colonies, which is 1.5–2 times more than in the prototype medium.

In tab. Figure 1 shows the intensity (E) of Clostridium perfringens on comparison media after 24 hours of cultivation at 37 ° C.

Table 1

Proposed Wednesday

25 4.0 2.5 Sulfur- 2.0 hydril25 5.0 3.0 amine amine- 3.2 Noananthra 4.0 4.0 2.5 quinone 2.8

ro25 4.0 2.52.8 per ha464 + 50 536 + 11 709 + 36

497 + 82

31

in tab. Figure 2 shows the intensity (E) of accumulation of Clostridium reg-ring cells on comparison media after 4 hours of culture at.

table 2

E (M + m) the number of cells in 0.1 ml dilution

223 + 13

120 + 20

As can be seen from the table. 2, the accumulation of clostridium biomass in the medium according to the invention after 4 hours of cultivation is 1.5-2 times more compared with the prototype medium.

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Claims (1)

Invention Formula Nutrient medium for clostridial effusion, containing D-glucose, sodium chloride, reductone, and miocontal broth, characterized in that, in order to increase the biomass yield, it contains a redox polymer with sulfhydryl groups in the following quantitative ratio of the components, g / l: 2.5-3.0 D-Glucose Sodium Chlo4, 0-5.0 H. Redox Polymer with Sulfhydryl 2,0-3,2 groups of M so-peptone Else broth