SU1275042A1 - Method of producing dry nutrient media - Google Patents

Abstract

The invention relates to microbiology and makes it possible to obtain dry nutrient media in a simplified way by carrying out processes of mixing the components, granulating, and drying with a single technical process in one apparatus. The components of the medium are loaded into a tank in which airflow is created. A granulating liquid preheated to 40-50 ° C is fed into the vortex layer: a protein hydrolyzate or a solution of polymer compounds with a molar mass of more than 500 in an amount of 150-350 mp per 1 kg of dry matter. The granular liquid serves sprayed air compressed to 0.25-0.35 MPa, and the components of the medium used in small quantities are introduced into the composition of the granulating liquid. At the end of the granulation process, also in an air stream (Lax, the medium is dried at 5070 ° C to a residual moisture content of 4-6%. 2zpf-ly. SP 4i tC

Description

This invention relates to microbiology, and more specifically to methods for producing dry culture media.

The purpose of the invention is to simplify and reduce the cost of the method.

The method is carried out as follows.

The prepared dry components of the nutrient medium are loaded into the food tank (dryer-granulator) with a perforated bottom, the hole diameter of which is designed for the finest grinding of solid matter. The suction fan creates the necessary airflow, which, passing through the bottom of the product tank from bottom to top, causes the product to become suspended. At the same time, due to the intense swirling of particles by the air flow, the product is mixed. The region of the velocity of the flow of the vortex medium, lying between the minimum vortex velocity and the velocity Tue of the removal, is chosen in such a way that the uniform vortex is carried out with insignificant losses of removal, without the formation of channels and impacts. For dry nutrient media, an optimum operating flow rate of 2.5–3 m / s can be chosen. Depending on the dispersed composition of the preparation, the duration of mixing of the components of dry nutrient media is 4-6 minutes.

Then, a granulating liquid in the amount of 150-350 ml per 1 kg of the dry preparation, sprayed with compressed air to 0.25-0i, 35 MPa, is irrigated by a pump through a two-material nozzle through a pump nozzle and irrigates the product, which is located in the fluidized bed. At the same time, around the smallest droplets, which are the centers of granulation, due to the collision and sticking of individual particles, the product agglomerates. For nutrient media, the most effective and firmly binding granulating liquids are protein hydrolysates and / or solutions of polymeric compounds 1 - substances with mol. weighing more than 500, having large branched macromolecules that allow the bonding of the surface of the particles to the distance of the length of the molecule to 1.2 nm.

At the end of the granulation, the liquid supply is stopped. Received by

422

the granules are dried in a fluidized bed with a heated stream of air at 50-70 ° C to a residual moisture content of 4-6%,

Example 1. Getting environment

Levin.

The components eivQcocTb of the dryer-granulator are loaded with components, kg: dry nutrient broth - 1,260, sucrose 1,263; agar-agar 1,000; lactose 0.830; sodium phosphate 0.07; sodium chloride 0,430, fodder yeast extract 0,100. A 6% solution of polyvinyl is used as a granulating liquid.

alcohol. To 750 ml of polyvinyl alcohol with a specified concentration, stirring, add the components that make up the medium in small quantities: eosin 0.039 and methylene blue

0,008 kg. To reduce the viscosity, the granulating liquid is heated to 40 ° C. The dry components are mixed with an air stream supplied at a speed of 3 m / s for 6 minutes. The resulting mixture, in a fluidized bed, is irrigated with a granulating liquid, finely sprayed compressed to 0.30 MPa by air, through a two-material nozzle with a flow rate of 50 ml /

/ min After 15 min, i.e. after the flow rate of 750 ml of granulating liquid, the flow is stopped. and the nozzle is blown with compressed air. The granulate is dried in the same apparatus in a fluidized bed heated to the air flow supplied at a speed of 3 m / s for 15 minutes to a residual moisture content of 5%. Total losses are 0.9%. The result is a 5.0 kg environment Levin.

Example 2. Getting environment Buchin.

Claims (3)

Components are loaded into the product capacity of the dryer-granule and kg: dry nutrient broth agaragar 0.85; sodium chloride 1.35, glucose 0.78. Pancreatic sprat hydrolyzate with a solids content of 25% is used as a granulating liquid. To 900 ml of the hydrolyzate with a temperature of 35 ° C, stirring, sequentially, the components that make up the medium in small quantities are introduced: quinosol 0.01 and. water blue 0.059 kg. Mix the dry components with an air stream supplied at a speed of 2.5 m / s for 5 minutes. The resulting mixture, which is in a fluidized bed, is irrigated with a granulating liquid, finely sprayed with compressed air up to 0.25 MPa, through a two-material nozzle at a flow rate of 45 ml / min. After 20 minutes, i.e. After consuming 900 ml of the granulating liquid, the feed is stopped and the bore is blown with compressed air. The granulate obtained is dried in the same apparatus in a fluidized bed, heated to 50 ° C with a stream of air supplied at a speed of 2.5 m / s for 15 minutes to a residual moisture content of 4%. Total loss is 0.8%. The result is 5.0 kg of Buchin medium. Example 3. Preparation of dry nutrient agar. Components are loaded into the product capacity of the dryer-granulator of a torus, kg: dry nutrient broth 1.71, agaragar 1.6 and sodium chloride 0.84. Pancreatic sprat hydrolyzate with a solids content of 50% is used as a granulating liquid. The sprat hydrolyzate in the amount of 1750 ml is heated to 50 ° C. Dry components are mixed in the product tank with an air stream supplied from a speed of 2.5 m / s for 4 minutes. The resulting mixture, which is in the fluidized bed, is irrigated with a granulating liquid, finely sprayed with compressed air to 0.35 MPa, through a two-material nozzle at a flow rate of 85 ml / min. After 21 minutes, i.e. after consumption of 1750 ml of the granulating liquid, the feed is stopped, and the nozzle is blown with compressed air. The resulting granulate is dried in the same apparatus in a fluidized bed heated to 60 ° C with an air stream supplied at a speed of 2.5 m / s for 18 minutes to a residual moisture content of 6%. Total losses are 0.5%. The result is 5.0 kg of dry nutrient agar. The invention provides a cheaper and simpler way, reduces losses, ensures an even distribution of the components of the medium. Claim 1. Method for the production of dry nutrient media, involving the mixing of agar-agar and powdered components with a wetting fluid, preheated to 40-50 ° C and finely divided, granulating and drying the resulting mass in a fluidized bed at 50-70 ° C, different the fact that, in order to simplify and reduce the cost of the method, the mixing of the medium components with the wetting fluid, the granulation and the subsequent drying are carried out sequentially in the fluidized bed, and the wetting liquid is introduced into the MP 150-350 honors per 1 kg of dry product at raspypenii air pressurized to 0.25-0.35 MPa, and the drying is carried out to a residual humidity of 4-6%. 2. The method according to claim 1, wherein the use of indicators, dyes, inhibitors, decomposers when used in the composition of the medium of microadditives, they are introduced into the wetting liquid. 3. The method according to claim 2, characterized in that the solutions of polymeric compounds with mol. weighing from 500 to 1 million or protein idrolizat.