SU1268567A1 - Method of producing ampholyte-carriers for isoelectric focusing of proteins - Google Patents
Method of producing ampholyte-carriers for isoelectric focusing of proteins Download PDFInfo
- Publication number
- SU1268567A1 SU1268567A1 SU843845379A SU3845379A SU1268567A1 SU 1268567 A1 SU1268567 A1 SU 1268567A1 SU 843845379 A SU843845379 A SU 843845379A SU 3845379 A SU3845379 A SU 3845379A SU 1268567 A1 SU1268567 A1 SU 1268567A1
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- SU
- USSR - Soviet Union
- Prior art keywords
- proteins
- isoelectric focusing
- hours
- carriers
- molar ratio
- Prior art date
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Abstract
Изобретение относитс к биохимии и может быть использовано при разделении смеси белков. Целью изобретени вл етс упрощение технологии процесса получени амфолитов-носителей дл изоэлектричеСкого фокусировани белков. Поставленна цель достигаетс взаимодействием а шнокислоты с этиленимином при их мол рном соотношении 1:5-8,8 при 50-100°С в течение 2-10 ч с последующей обработкой полученного продукта смесью непрес S дельных карбоновьк кислот при мол рном соотношении аминокислЬты и не (Л предельной кислоты 1:1-5 при 50-100 4 с в течение 5-20 ч.This invention relates to biochemistry and can be used in the separation of a mixture of proteins. The aim of the invention is to simplify the process of obtaining ampholytes carriers for isoelectric focusing of proteins. This goal is achieved by the interaction of amino acids with ethyleneimine at their molar ratio of 1: 5-8.8 at 50-100 ° C for 2-10 hours, followed by treatment of the resulting product with a mixture of non-fresh S carboxylic acids at a molar ratio of amino acid and not (L limit acid 1: 1-5 at 50-100 4 s for 5-20 hours
Description
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Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU843845379A SU1268567A1 (en) | 1984-12-14 | 1984-12-14 | Method of producing ampholyte-carriers for isoelectric focusing of proteins |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU843845379A SU1268567A1 (en) | 1984-12-14 | 1984-12-14 | Method of producing ampholyte-carriers for isoelectric focusing of proteins |
Publications (1)
Publication Number | Publication Date |
---|---|
SU1268567A1 true SU1268567A1 (en) | 1986-11-07 |
Family
ID=21159165
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SU843845379A SU1268567A1 (en) | 1984-12-14 | 1984-12-14 | Method of producing ampholyte-carriers for isoelectric focusing of proteins |
Country Status (1)
Country | Link |
---|---|
SU (1) | SU1268567A1 (en) |
-
1984
- 1984-12-14 SU SU843845379A patent/SU1268567A1/en active
Non-Patent Citations (1)
Title |
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Выложенна за вка DE № 2814408, кл. С 07 С 103/52, 1978. * |
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