SU1268567A1 - Method of producing ampholyte-carriers for isoelectric focusing of proteins - Google Patents

Method of producing ampholyte-carriers for isoelectric focusing of proteins Download PDF

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Publication number
SU1268567A1
SU1268567A1 SU843845379A SU3845379A SU1268567A1 SU 1268567 A1 SU1268567 A1 SU 1268567A1 SU 843845379 A SU843845379 A SU 843845379A SU 3845379 A SU3845379 A SU 3845379A SU 1268567 A1 SU1268567 A1 SU 1268567A1
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SU
USSR - Soviet Union
Prior art keywords
proteins
isoelectric focusing
hours
carriers
molar ratio
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Application number
SU843845379A
Other languages
Russian (ru)
Inventor
Андрей Петрович Мурель
Аго Антсович Конгас
Светлана Владимировна Вильде
Оскар Георгиевич Киррет
Марет Савельевна Панк
Original Assignee
Ордена Трудового Красного Знамени Институт Химии Ан Эсср
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Priority to SU843845379A priority Critical patent/SU1268567A1/en
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Publication of SU1268567A1 publication Critical patent/SU1268567A1/en

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Abstract

Изобретение относитс  к биохимии и может быть использовано при разделении смеси белков. Целью изобретени   вл етс  упрощение технологии процесса получени  амфолитов-носителей дл  изоэлектричеСкого фокусировани  белков. Поставленна  цель достигаетс  взаимодействием а шнокислоты с этиленимином при их мол рном соотношении 1:5-8,8 при 50-100°С в течение 2-10 ч с последующей обработкой полученного продукта смесью непрес S дельных карбоновьк кислот при мол рном соотношении аминокислЬты и не (Л предельной кислоты 1:1-5 при 50-100 4 с в течение 5-20 ч.This invention relates to biochemistry and can be used in the separation of a mixture of proteins. The aim of the invention is to simplify the process of obtaining ampholytes carriers for isoelectric focusing of proteins. This goal is achieved by the interaction of amino acids with ethyleneimine at their molar ratio of 1: 5-8.8 at 50-100 ° C for 2-10 hours, followed by treatment of the resulting product with a mixture of non-fresh S carboxylic acids at a molar ratio of amino acid and not (L limit acid 1: 1-5 at 50-100 4 s for 5-20 hours

Description

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Claims (1)

Формула изобретенияClaim Способ получения амфолитов-носителей для изоэлектрического фокусирования белков путем взаимодействия аминокислот с органическим соединением, отличающийся тем, что, с целью упрощения технологии процесса, в качестве органического соединения используют этиленимин и взаимодействие проводят при молярном соотношении аминокислоты и этипеними— на 1:5-8,8 при 50—100 6С в течение 2— 10 ч с последующей обработкой полученного продукта смесью непредельных карбоновых кислот при молярном соотношении аминокислоты и непредельной кислоты 1:1-5 при 50-100°С в течениеA method of producing carrier ampholytes for isoelectric focusing of proteins by the interaction of amino acids with an organic compound, characterized in that, in order to simplify the process technology, ethyleneimine is used as an organic compound and the interaction is carried out at a molar ratio of amino acid to etipenim of 1: 5-8, 8 6 at 50-100 C for 2- 10 hours, followed by treating the resultant product with a mixture of unsaturated carboxylic acids at a molar ratio of amino acid and unsaturated acid of 1: 1-5 at 50-100 ° C. flow 5-20 ч.5-20 hours ВНИИПИ Заказ 5991/23 Тираж 379 · ПодписноеVNIIIPI Order 5991/23 Circulation 379 · Subscribed Произв.-полигр. пр-тие, г. Ужгород, ул. Проектная, 4Custom polygr. ave, city of Uzhhorod, st. Project, 4
SU843845379A 1984-12-14 1984-12-14 Method of producing ampholyte-carriers for isoelectric focusing of proteins SU1268567A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SU843845379A SU1268567A1 (en) 1984-12-14 1984-12-14 Method of producing ampholyte-carriers for isoelectric focusing of proteins

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SU843845379A SU1268567A1 (en) 1984-12-14 1984-12-14 Method of producing ampholyte-carriers for isoelectric focusing of proteins

Publications (1)

Publication Number Publication Date
SU1268567A1 true SU1268567A1 (en) 1986-11-07

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Family Applications (1)

Application Number Title Priority Date Filing Date
SU843845379A SU1268567A1 (en) 1984-12-14 1984-12-14 Method of producing ampholyte-carriers for isoelectric focusing of proteins

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SU (1) SU1268567A1 (en)

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Выложенна за вка DE № 2814408, кл. С 07 С 103/52, 1978. *

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