SU125882A1 - Installation for working with aerosols - Google Patents

Description

Existing installations for aerosol contamination and inhalation of vaccines and drugs by animals can only be used for small experimental animals.

The installation proposed by the visitor is devoid of this significant drawback and is structurally different from the existing ones in that it uses a gas mask equipped with a filter and breathing tube that connects the gas mask to the camera. This achieves the possibility of conducting experiments on the contagion and inhalation of microbial suspensions, vaccines and medications for large animals.

FIG. 1 shows the installation diagram; in fig. 2 - general view of the camera; in fig. 3-mount hatch cover Calgier.

The installation consists of a mask gas mask 1, a gas mask filter 2, a breathing gas mask tube 3, a chamber 4, an air pump 5 valve, a spray gun 6, an air hose from the compressor 7, a compressor 8, an intake hose for the compressor 9, an air suction hose 10, a hose, connecting the installation with a gas meter 11, a gas meter 12 and a gas intake tube 13.

The basis of the installation is a camera made of organic glass (plexiglass) with a thickness of 8-10 mm.

In the front wall of the chamber there is a hatch 14, through which small animals are placed in the chamber in cages or freely. The lid has a rubber gasket 15 and is hermetically fastened to the chamber wall with bolts 16. In the right wall, closer to the base, a circular hole 17 is cut out, around which two Plexiglas rings are pasted one on top of the other. On the outer ring, which has a somewhat larger circumference, the edge of a rubber glove with a long cuff is tightened and strengthened. In the same wall, near the bottom of the chamber, a hole 18 is drilled into which a hose is inserted to drain excess air from the chamber into a vessel with a disinfectant solution in an anti-gas box or

M 125862

muffle furnace. There is a hole in the upper wall of the chamber for the thermometer 19, and in the back a hole for the open water pressure gauge.

The sprayer is mounted on a special bracket in the upper part of the rear wall of the chamber. The aerosol from the sprayer enters the chamber through a short rubber-EO tube. The compressor with its outlet is connected via a rubber hose to the sprayer and the inlet to the gas meter.

It is enough these elements when carrying out experiences with small laboratory animals, hinder, and in the camera.

For experiments on large animals, the installation provides the following. At the bottom of the chamber, closer to the front wall, there is a version in which a corrugated breathing gas mask is inserted, going to the gas mask worn on the animal's face. Valve mask 21 opens when inhaling. When exhaling, the air exits through a gas mask filter that has exhalation valves 22.

The missing amount of air for a large animal; into the chamber (when the animal inhales) through an additional air suction hose connected by a tee to a hose going from the gas meter to the compressor. The air inlet is at the bottom of the chamber and has an intake valve.

For small animals, the breathing tube of the gas mask is left closed, and excess aerosol (air) is removed from the chamber with a hose to drain excess air into one of the above-mentioned disinfectants.

A suspension of microbes, vaccines, etc. in the calculated concentrations is poured into the nebulizer and connected to the compressor and to the chamber. Small animals, nutrient media and other objects are placed inside the chamber, the bolts of the hatch cover are tightened tightly, and then the compressor is turned on. The aerosol enters the chamber in the form of a grayish cloud. -. The walls of the chamber remain dry. Manometer pressure should not exceed 0.5-1 cm of water column.

Spraying the calculated amount of suspension (solution) and maintaining the desired exposure, the compressor is turned off. The atomizer opening is closed with a rubber stopper from the inside of the chamber, the atomizer is separated and the chamber is flushed with fresh air using a compressor. The test animals are then removed from the camera, and the camera is disinfected by closing all outlets.

A rubber stopper is removed from the orifice of the breathing tube of the gas mask for placing small animals on large animals. The gas mask is worn on the subject of the experimental animal, while ensuring that the obturator fits tightly to the lower part of the muzzle, and strengthens the mask on the head with the help of special belts. Animals are fixed in the appropriate machines.

Make sure that the animal is well fixed and the suction valve is working right (the petal is raised when inhaling), turn on the compressor and spray the required amount of suspension of the solution.

At the end of the experiment, the mask of the gas mask is separated from the breathing tube of the gas mask and immersed in the disinfectant solution. The chamber is flushed with fresh air and disinfected with formalin vapors, ethylene oxide or others. To neutralize formalin vapors, the chamber is treated with ammonia. Mask gas mask is disinfected in the chamber in the same way and air. The animal's face is treated with a disinfector.

When working with bacterial sprays, the rules of personal prophylaxis are observed: protective clothing, gas mask and rubber gloves are worn.

In the process of repeated experiments for more than two years, it was found out that the installation fully corresponds to the purpose.

Subject invention

Installation for working with aerosols, consisting of a gas meter, a compressor, a chamber and hoses, characterized in that, in order to carry out experiments and carry out inhalation of microbial suspensions, vaccines and medicament drugs by large animals, a gas mask is used, equipped with a filter and breathing tube connecting the gas mask to the camera.