SU1255054A3 - Method of producing n-nitrophenyl-alpha-maltoheptaozide - Google Patents

Method of producing n-nitrophenyl-alpha-maltoheptaozide Download PDF

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Publication number
SU1255054A3
SU1255054A3 SU802903252A SU2903252A SU1255054A3 SU 1255054 A3 SU1255054 A3 SU 1255054A3 SU 802903252 A SU802903252 A SU 802903252A SU 2903252 A SU2903252 A SU 2903252A SU 1255054 A3 SU1255054 A3 SU 1255054A3
Authority
SU
USSR - Soviet Union
Prior art keywords
alpha
nitrophenyl
amylase
producing
maltoheptaozide
Prior art date
Application number
SU802903252A
Other languages
Russian (ru)
Inventor
Раушер Элли
Нойманн Ульрих
Вильхельм Валефельд Аугуст
Хаген Александер
Грубер Вольфганг
Цигенхорн Иоахим
Шаих Ойген
Денеке Ульферт
Михал Герхард
Вайманн Гюнтер
Original Assignee
Берингер Маннхайм Гмбх (Фирма)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Берингер Маннхайм Гмбх (Фирма) filed Critical Берингер Маннхайм Гмбх (Фирма)
Application granted granted Critical
Publication of SU1255054A3 publication Critical patent/SU1255054A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C12Q2334/10—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases p-Nitrophenol derivatives

Abstract

A substrate for the determination of alpha -amylase of the formula I is prepared by reacting the particular phenyl glucoside or nitrated phenyl glucoside with alpha -cyclodextrin, amylase or soluble starch in the presence of Bacillus macerans amylase. The radical R in the formula I has the meaning stated in the claim. <IMAGE>

Description

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Изобретение относитс  к способам получени  производ агх Сахаров, в частности оптически чистого и нит- рофеиил-й-мальтогептаозида..The invention relates to methods for the preparation of sugar and sugar production, in particular, optically pure and nitropheyl-y-maltoheptaoside.

Цель изобретени  - получение оптически чистого продукта, а следовательно , создание возможности прос того определени  и -амилазы в биоло- .гических жидкост х, таких как гепаринова  плазма, моча, сьшоротка кро ви.The purpose of the invention is to obtain an optically pure product and, therefore, to enable the determination of i-amylase in biologic fluids, such as heparin plasma, urine, and the shortening of blood.

Пример, 680 мг амилазы Ва- cillus macerans (Е,С,2,4.1.19 из Bac,mac,DSM-24) - лиофилиза т (навеска Oi46 V/MF, содерл ание белка в насеске 28,5%, свободна от активностей, расщепл юРедактор А,0гар Заказ 4733/60Example, 680 mg of bacillus macerans amylase (E, C, 2.4.1.19 from Bac, mac, DSM-24) - lyophilis t (weight Oi46 V / MF, protein content in the collection is 28.5%, free from activities, split the editor, A 0gar Order 4733/60

Составитель 10, Голова Техред И.Верес Compiled by 10, Head of Techred I.Veres

Корректор Corrector

Тираж 470ПодписноеCirculation 470 Subscription

BlllfflOIi Государственного комитета СССРBlllfflOIi USSR State Committee

по делам изобретений и открытий 113035, Москва, Ж-355 ТауБ1Ска  наб., д.4/5for inventions and discoveries 113035, Moscow, Zh-355 TauB1Ska nab., 4/5

Производственно-полиграфическое предпри тие, г.Ужгоррд, ул.Проектна , 4Production and printing company, Uzhgorrd, Project St., 4

25505422550542

щих п-ннтрофенил-а;-В-гл окозид) смешивают с 400 мгсс-П-п-нитрофенил- глюкозида, 3,5 г сб -цикло декстрина и 70 мл 0,01 М фосфатного буфера Со- 5 ренсена, рН 6,2, Смесь инкубируют 24 ч при 37 С. Образовавшийс  декстрин обрабатывают трюшорэтанолом и отдел ют тетрахлорэтиленовое производное .p-n-ntrophenyl-a; -B-gl okozid) is mixed with 400 mg cc-P-p-nitrophenyl glucoside, 3.5 g of sb-cyclodextrin and 70 ml of 0.01 M Co-5 rensen phosphate buffer, pH 6 2 The mixture is incubated for 24 hours at 37 ° C. The dextrin formed is treated with tryuschol ethanol and the tetrachlorethylene derivative is separated.

10ten

Продукт хроматографируют на сшитом дектране (Сефадекс LH20), лиофи- лизуют и получают 50 мг лиофилиза- та 1 -нитрофенил-сС-мальтогептаозида. Т.пл. (разлож.); cijjj. 194,6°; степень чистоты 99,5% (по данным HPLC).The product is chromatographed on a crosslinked sectane (Sephadex LH20), lyophilized, and 50 mg of 1-nitrophenyl-c-maltoheptaoside lyophilisate are obtained. M.p. (decomposed); cijjj. 194.6 °; 99.5% purity (according to HPLC).

Корректор Л.Ш-шипенкоProofreader L.Sh-shipenko

Claims (1)

СПОСОБ ПОЛУЧЕНИЯ η -НИТРОФЕНИЛ-Л-МАЛЬТОГЕПТАОЗИДА общей формулы где R - и -нитрофенилглюкозид, отличающийся тем, что h-нитрофенилглюкозид приводят во взаимодействие с ρύ -циклодекстрином ’ <9 в присутствии амилазы Bacillus tnacerans при pH 6,2.METHOD FOR PRODUCING η-NITROPHENYL-L-MALTOGEPTAOOSIDE of the general formula where R is and -nitrophenylglucoside, characterized in that h-nitrophenylglucoside is reacted with ρύ -cyclodextrin ’<9 in the presence of Bacillus tnacerans amylase at pH 6.2. >> CHCH
SU802903252A 1977-12-14 1980-04-07 Method of producing n-nitrophenyl-alpha-maltoheptaozide SU1255054A3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19772755803 DE2755803A1 (en) 1977-12-14 1977-12-14 METHOD AND REAGENT FOR DETERMINING ALPHA-AMYLASE

Publications (1)

Publication Number Publication Date
SU1255054A3 true SU1255054A3 (en) 1986-08-30

Family

ID=6026144

Family Applications (2)

Application Number Title Priority Date Filing Date
SU782662348A SU1212332A3 (en) 1977-12-14 1978-09-04 Method of identifying alpha-amylase
SU802903252A SU1255054A3 (en) 1977-12-14 1980-04-07 Method of producing n-nitrophenyl-alpha-maltoheptaozide

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SU782662348A SU1212332A3 (en) 1977-12-14 1978-09-04 Method of identifying alpha-amylase

Country Status (6)

Country Link
CH (1) CH650800A5 (en)
CS (2) CS236751B2 (en)
DE (1) DE2755803A1 (en)
ES (1) ES480029A1 (en)
HU (1) HU189610B (en)
SU (2) SU1212332A3 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4472499A (en) * 1982-01-22 1984-09-18 American Hoechst Corporation Reagents for the determination of enzymes
DE3323245A1 (en) * 1983-06-28 1985-01-10 Merck Patent Gmbh, 6100 Darmstadt METHOD AND REAGENT FOR DETERMINING (ALPHA) AMYLASE
DE3328616A1 (en) * 1983-08-08 1985-02-28 Boehringer Mannheim Gmbh, 6800 Mannheim OLIGOGLUCOSIDE DERIVATIVES

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL179399C (en) * 1976-07-13 1986-09-01 Du Pont METHOD FOR DETERMINING THE AMYLASE CONTENT OF A SAMPLE AND REAGENT TEST PACKAGE THEREFOR.
US4102747A (en) * 1977-07-28 1978-07-25 American Hospital Supply Corporation Amylase determination
DE2752501A1 (en) * 1977-11-24 1979-05-31 Kurt Prof Dr Wallenfels Alpha-d-malto:dextrin derivs. as indicators - for colorimetric analysis of amylase(s)

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Патент DE № 2662348, кл. С 08 В 37/00, опублик. 1978. *

Also Published As

Publication number Publication date
HU189610B (en) 1986-07-28
ES480029A1 (en) 1980-03-01
DE2755803A1 (en) 1979-06-21
CS236758B2 (en) 1985-05-15
SU1212332A3 (en) 1986-02-15
CS236751B2 (en) 1985-05-15
CH650800A5 (en) 1985-08-15
DE2755803C2 (en) 1990-03-15

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