SU1249416A1 - Method of estimating efficiency of cryogenic action on biological tissue - Google Patents
Method of estimating efficiency of cryogenic action on biological tissue Download PDFInfo
- Publication number
- SU1249416A1 SU1249416A1 SU853884654A SU3884654A SU1249416A1 SU 1249416 A1 SU1249416 A1 SU 1249416A1 SU 853884654 A SU853884654 A SU 853884654A SU 3884654 A SU3884654 A SU 3884654A SU 1249416 A1 SU1249416 A1 SU 1249416A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- bioassay
- bound water
- free
- sample
- ratio
- Prior art date
Links
Abstract
Изобретение относитс к радиоспектроскопии и может найти применение в медицине при проведении криохирургических операций. Способ повышает точность и надежность оценки эффективности криовоздействи . С поверхности биообъекта, подвергающегос замораживанию, берут биопробу, опускают ее в ампулу, которую помещают в зазор посто нного магнита экспресс - анализатора дерного магнитного резонанса, Задава последовательности из двух импульсов, по экспоненциальному спаду эхо-сигнала определ ют врем Т 2 спин-спиновой релаксации, характеризующее степень св занности воды и биоткани с гидрофильными центрами биологических макромолекул и других веществ, вход щих в состав биоткани. Постепенно охлажда биопробу до -180°С, из зависимости I/Т определ ют соотношение свободной и св занной воды биоткани. После отогрева биообъекта вновь берут пробу. Сравнивают соотношение свободной и св занной воды биопробы после обогрева с первоначальным анализом. Отсутствие св занной воды в пробе свидетельствует о необратимом некрозе ткани . с 4 и«л О5The invention relates to radiospectroscopy and can be used in medicine during cryosurgical operations. The method improves the accuracy and reliability of the evaluation of the effectiveness of cryotherapy. A bioassay is taken from the surface of a bio-object under freezing, immersed in an ampule which is placed in the permanent magnet gap of an express nuclear magnetic resonance analyzer. Given a sequence of two pulses, the T 2 spin-spin relaxation time is determined by the exponential decrease of the echo signal characterizing the degree of water and biotissue connectivity with hydrophilic centers of biological macromolecules and other substances that make up biotissue. Gradually cooling the bioassay to -180 ° C, the ratio of free and bound water of biotissue is determined from the I / T dependence. After warming the bio-object again take a sample. The ratio of free and bound water bioassay after heating is compared with the initial analysis. The absence of bound water in the sample indicates irreversible tissue necrosis. with 4 and "l O5
Description
Изобретение относитс к ое.р: юс1:ек pocKonHHj а именно к H,;i;epHOi iy члгвк г ному резонансу (ЯМР), л могкнт чайти применение в медицине при цзоведении криохирургических операций,The invention relates to o.r.: yus1: ek pocKonHHj, namely to H,; i; epHOi iy of the resonant resonance (NMR), which can be used in medicine in cryosurgery,
Цель изобретени - ::1овы1:цение то-:- ности и надежности оценки .эффективно сти криовозцействи ,The purpose of the invention is :: 11: the value of that is: - - ness and reliability of the evaluation of the efficiency of cryo-recovery,
Способ осу1цествпкет|;: сларл юдим образомWay osu1stsevpket | ;: slar yudim way
С поверхности биообъекта, подвергающегос закораживанию, барут бно- пробуэ опускают ее Е ампулv, которую помещают в зазор посто нно-: : магнита эксгфесс-анализатора ЖР, За азаксь последо:вательност к: ;: и, дв ; кмпульс о в 5 по 3к сп о и е иптл an: ь н ому . -. 11 ад у э х о - сигнала определ ют врем i г:ин-чнино вой релаксации Т.,,, характЕр1-1э -ю и:и: степень св занности воды ЁУОТУЯНИ с гидрофильньЕМИ центрами биолог.;ческих макромолекул и друт х ве;дг:С ::-, ::ixo-- д ивдх в состав биоткани,, cci-c rieHiro охлажда биопробу до -iBO C, :з зависимости /Т,., определ ют сооттюши- ние свободной и св занной воды ткани.From the surface of the bio-object, which is subject to freezing, the barboo-probue is lowered by its E ampulev, which is placed in the gap permanently:: the magnet of an ex- test analyzer ЖР, For the following sequence: for::: and, dv; Kmpulis about 5 to 3k sp o and e ipl an: nd nom. -. 11 he ad ex o - the signal determines the time i g: in-relaxation T. ,,, characteristic 1-1-th and: and: the degree of water connection of YOWOTYUYANI with hydrophilic centers of biologic; macromolecules and druses ; dg: C :: -, :: ixo-- d i vdh into biotissue composition, cci-c rieHiro cooling biosample to iBO C,: Dependences / T,., determine the matching of free and bound water of tissue .
Врем анализа составл ет ; уп-т., На основе полученги:й инфорнглдигГ выбирают нео5ходил,1ьш т;ежи1 - кр ---: г;о - - действи на биообъект,,The analysis time is; up-t., On the basis of the obtained: th infornging digger choose neohodil, 1sh t; hedgehog1 - cr ---: g; o - - effect on the biological object,
После отогрева Еиосбьевта -;-овь берут пробу и повтор кт в последовательнос-и прОцеду: Л ;;:: anv:-- за. Сравнивают соотношение :::нобод;:ой и св занной воды биопробь; iocjie отогрева первоначалычъпч ааа у-зок. Прг:: тическое отсутствие сн заг чой , тироое сцидетелтл пгует о neof paTi (екрозе ткани, HCKJ Hi4aK)i;:eM SOTMI iiccTb гюсстановлени жизнедс Я-:-Р: стч r:crji{ их о : 0 пев ,After warming Eosobiev -; - Ov take a sample and repeat the procedure in a sequential procedure: L ;; :: anv: - for. Compare the ratio of ::: foot ;, oh and bound water bioprobes; iocjie warming the initial stream aaa y-sok. Prg. Absence of cramping, tyroe stsideteltl puget about neof paTi (tissue eccrose, HCKJ Hi4aK) i;: eM SOTMI iiccTb russetting I -: - R: st r r
П р и м е Р; Крчо;:оздсй :.тз11 :за эгают о::1ухсль - к tpin-n c:-r. Лт,- ; м ризитую трем :;сдом:з тньп- жи-:)- :;; чистой линии,:.PRI m e R; Krcho;: click: tz11: go about :: 1хсл - to tpin-n c: -r. Lt, -; I have three embraces:; sdom: s tnpzhi -:) -: ;; clean line,:.
Анализ вз той пробь г:ока : а( соотношение ,пной ;н с i-: м ;.:-.чт чоды биоткани -(. 7- :ПAnalysis of the sample taken: o: a (ratio, pnou; n with i-: m;.: -. What is biotissue - (. 7-: P
Выбраны 3 г)с;:.има ь;риг;пс:5Ле-::; -- объэкт - охл );а:зии С-; ;---л;; ,:Selected 3 d) s;: .image; rig; ps: 5Le - ::; - obekt - okhl); a: zii S-; ; --- l ;; ,:
-30,, д(5 -60 1 до ---)(; ( ,-30 ,, d (5 -60 1 to ---) (; (,
Ана:1из проэь) нос,пр cnorD; ;:;- : 1 ;а; ывает соотг:оЕ1ен 5е с::воб;-::мг)й ; сн; -;анной нодь :--5о - :с --:: Ji,j; i ,5 J 93; 5 ,.Ana: 1 out of the nose, pr cnorD; ;:; -: 1; a; It corresponds to the following: OE1en 5e with :: Wob; - :: mg) nd; sn; -; Announcement: - 5о -: s - :: Ji, j; i, 5 J 93; five ,.
Нз cooTi rj iv-;ri,: ,j ; ,: c:if- ii ,I(;лy::: io- .те : 5C- :( м i : - Mbl i nooiiocc и ;К,И,; i f:i :i)r, ;cH C ;киз.:1г:д. .-; т -л I v {- / f iНз cooTi rj iv-; ri ,:, j; ,: c: if- ii, I (; ly ::: io- .te: 5C- :( m i: - Mbl i nooiiocc and; K, I,; if: i: i) r,; cH C; Key.: 1g: d. .-; t-l I v {- / fi
I и :I and:
о-nito-nit
криочоздсгистз: , ;,л:::,:;е. т:-:Cryocarbons:,;, l :::,:; e. t: -:
iHf свободно;- i-: f; ЧК г а ДПОЙiHf is free; - i-: f; CHK g and DPM
: : :ани до Kpno: o nj(ei r TBV;H i i:ie:3a методом :и(. а- crn ма рс чо анса, к по сгк: jir:-:i;cH свободно ; sr : ;: j;-H i;-:i п 5{1;фпктинН,)СТ1 i::: ani to Kpno: o nj (ei r TBV; H ii: ie: 3a by the method: and (. a-crn ma apc chos ans, k by cgc: jir: -: i; cH is free; sr:;: j; -H i; -: i p 5 {1; phptinH,) CT1 i
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU853884654A SU1249416A1 (en) | 1985-03-19 | 1985-03-19 | Method of estimating efficiency of cryogenic action on biological tissue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU853884654A SU1249416A1 (en) | 1985-03-19 | 1985-03-19 | Method of estimating efficiency of cryogenic action on biological tissue |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU1249416A1 true SU1249416A1 (en) | 1986-08-07 |
Family
ID=21173285
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU853884654A SU1249416A1 (en) | 1985-03-19 | 1985-03-19 | Method of estimating efficiency of cryogenic action on biological tissue |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU1249416A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5433717A (en) * | 1993-03-23 | 1995-07-18 | The Regents Of The University Of California | Magnetic resonance imaging assisted cryosurgery |
-
1985
- 1985-03-19 SU SU853884654A patent/SU1249416A1/en active
Non-Patent Citations (1)
| Title |
|---|
| Электронна промышленность, 1982 №. 8, с. 79-80. Фаррар Т. и Беккер Э. Импульсна и .Фурье-спектроскопи ЯМР, - М.: Мир, ,1973, с. 4I-49. * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5433717A (en) * | 1993-03-23 | 1995-07-18 | The Regents Of The University Of California | Magnetic resonance imaging assisted cryosurgery |
| US5706810A (en) * | 1993-03-23 | 1998-01-13 | The Regents Of The University Of California | Magnetic resonance imaging assisted cryosurgery |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Messick | Hematodinium perezi infections in adult and juvenile blue crabs Callinectes sapidus from coastal bays of Maryland and Virginia, USA | |
| Eom et al. | Morphologic descriptions of Taenia asiatica sp. n | |
| Nasidi et al. | Urban yellow fever epidemic in western Nigeria, 1987 | |
| Wong et al. | High prevalence of human skeletal muscle sarcocystosis in south-east Asia | |
| Gunasekaran et al. | Effects of extracorporeal shock wave lithotripsy on the structure and function of rabbit kidney | |
| Moksness et al. | Ageing and back-calculating growth rates of Pacific herring, Clupea pallasii, larvae by reading daily otolith increments. | |
| SU1249416A1 (en) | Method of estimating efficiency of cryogenic action on biological tissue | |
| Sex | Determining age, sex, and reproductive condition | |
| Yourth et al. | On understanding variation in immune expression of the damselflies Lestes spp. | |
| Kingscote et al. | Diseases of wapiti utilizing cattle range in southwestern Alberta | |
| Koeck et al. | Marked loss of sympathetic nerve fibers in chronic Charcot foot of diabetic origin compared to ankle joint osteoarthritis | |
| Nicol et al. | Accumulation of fluorescent age pigments in a laboratory population of Antarctic krill Euphausia superba Dana | |
| Moore | The detection of enteric carriers in towns by means of sewage examination | |
| WEHNERT et al. | In vivo and in vitro studies on the host specificity of Trypanoplasma salmositica | |
| Broughton et al. | Histological differences in the lateral musculature of O‐group roach, Rutilus rutilus (L.) from different habitats | |
| Shamseldean et al. | Romanomermis culicivorax: penetration of larval mosquitoes | |
| Overstreet et al. | Mesenchymal tumors of some estuarine fishes of the northern Gulf of Mexico. II. Subcutaneous fibromas in the southern flounder, Paralichthys lethostigma, and the sea catfish, Arius felis | |
| Cozzi et al. | Diving adaptations of the cetacean skeleton | |
| Frizzell et al. | Ultrasonically induced intravascular streaming and thrombus formation adjacent to a micropipette | |
| Xu et al. | Evaluation of a cohabitation challenge model in immunization trials for channel catfish Ictalurus punctatus against Ichthyophthirius multifiliis | |
| Yeremiev et al. | Histomorphological characteristics of Ascaris lumbricoides and Taeniarhynchus saginatus | |
| Tanaka | Mini-review: the ultrasonographical and serological chanbges and their improvement after praziquantel treatment Schistosoma japonicum infected patients in Leyete, Philippines | |
| Schantz | Immunodiagnostic tests with Echinococcus antigens in sheep with homologous and heterologous larval cestode infections | |
| Wagener et al. | Experimental amoebiasis in cats from acute and chronic human cases | |
| Mays | Serological methods of diagnosis of cancer with special reference to the gruskin test |