SU1242803A1 - Method of determining lactate concentration - Google Patents
Method of determining lactate concentration Download PDFInfo
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- SU1242803A1 SU1242803A1 SU843727803A SU3727803A SU1242803A1 SU 1242803 A1 SU1242803 A1 SU 1242803A1 SU 843727803 A SU843727803 A SU 843727803A SU 3727803 A SU3727803 A SU 3727803A SU 1242803 A1 SU1242803 A1 SU 1242803A1
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Abstract
Изобретение относитс к исследованию материалов при помощи электрических средств. Способ основан на .электролизе исследуемого раствора, в который введен медиатор, при 20- 25 С с введенным в него цитохромом В, в фосфатном буферном растворе рН которого 7,0(-7,5. С целью увеличени чувствительности способа электролиз провод т в присутствии лактатдегидро- геназы и восстановленного никотина- мидадениндинуклеотида в концентраци х , соответствующих предельному току . Это приводит к циклическому превращению лактата в биоферментной системе при участии медиатора, что приводит к увеличению тока ферментного электрода и соответственно к увеличению чувствительности способа. I табл. с о (ПThe invention relates to the study of materials using electrical means. The method is based on electrolysis of the test solution into which the mediator is introduced, at 20-25 ° C with cytochrome B introduced into it, in a phosphate buffer solution whose pH is 7.0 (-7.5. To increase the sensitivity of the method, electrolysis is carried out in the presence of lactate dehydrogenase and reduced nicotinamide adenine dinucleotide in concentrations corresponding to the current limit. This leads to cyclic transformation of lactate in the biofermental system with the participation of the mediator, which leads to an increase in the current of the enzyme electrode and GOVERNMENTAL to increase the sensitivity of the method. I Table. with a (P
Description
Изобретение относитс к области исследовани материалов с помощью электрических средств путем измерени тока ф(1рментных реакций при элек ролизе и может быть использовано при определении концентрации лактата в крови, микробиологических и биологических растворах.The invention relates to the field of materials research using electrical means by measuring the current f (1 fast reactions during electrolysis) and can be used to determine the concentration of lactate in the blood, microbiological and biological solutions.
Цель изобретени - увеличение чувствительности способа определе:ни концентрации латката.The purpose of the invention is to increase the sensitivity of the method defined: no latcate concentration.
Эти превращени ил.пюстрируютс следующей схемой:These transformations are illustrated by the following scheme:
цитохром Вcytochrome b
Л и- Fe П + НАДНLI- Fe P + NADH
Fe - е где ЛFe - e where L
1+1+
П + Fe ЛДГP + Fe LDG
Л + НАД электрохимически L + OVER Electrochemically
FeFe
лактат; П - пируват; ДДГ - НДЦ зависима лактатдегидрогеназа.lactate; P - pyruvate; FGD - NDC dependent lactate dehydrogenase.
Дл ос5 ществлени способа примен ют ферментный электрод, анод которого изготовл ют из стеклоуглерода с нанесенным на него цитохромом J и лактатдегидрогеназой, покрытый ди.злизной ме мбранойэ а катод - на серебра, покрытого AgCl.To implement the method, an enzyme electrode is used, the anode of which is made of glass carbon with cytochrome J deposited on it and lactate dehydrogenase, coated with membrane mucosa, and the cathode - on silver coated with AgCl.
Пример 1. Электролиз исследуемого раствора провод т в присутствии цитохрома В, концентраци которого 60 ед/мл, НДЦ зависимой лактатдегидрог1гназы , концентраци которой 60 ед/мЛ; 5 М1%М лактата в (); М фосфатном буфере. Определение йави- симости вел| Мины тока от концентрации НАДН ведут при рН 7,5, OB относительно Ag/AgCl.Example 1. The electrolysis of the test solution is carried out in the presence of cytochrome B, the concentration of which is 60 units / ml, NDC-dependent lactate dehydrogen hormone, the concentration of which is 60 units / ml; 5 M1% M lactate in (); M phosphate buffer. Definition of yaviimosti led | The current mines from the concentration of NADH are carried out at pH 7.5, OB relative to Ag / AgCl.
Зависимость величины тока от концентрации НАДН приведена в таблице .The dependence of the current on the concentration of NADH is given in the table.
Составитель И, Рогаль 1 едактор А. Шишкина Техред Н.Бонкало Корректор . ШарошиCompiled by And, Rogal 1 edaktor A. Shishkina Tehred N. Bonkalo Corrector. Sharoshi
Заказ 3695/40 Тираж 778Г одписноеOrder 3695/40 Circulation 778G single
ВНИИПИ Государственного комитета СССРVNIIPI USSR State Committee
по делам изобретений и открытий П30355 Москва, Ж-35, Раушска наб,, д, 4/5for inventions and discoveries P30355 Moscow, Zh-35, Raushsk nab ,, d, 4/5
Производственно-полиграфическое предпри тие, г, З жгород, ул. Проектна , 4Production and printing company, G, Z zhgorod, st. Project, 4
Из таблицы видно, что наибольша величина тока (предельный), т.е. 18 нА, наблюдаетс при концентрации 1 мМ, а при дальнейшем увел1-гчении концент)зации. НАДН величина тока снижаетс ,.It can be seen from the table that the current is the highest (the limiting), i.e. 18 nA, is observed at a concentration of 1 mM, and with further increase in concentration concentration. NADH current magnitude decreases,.
Предлагаемое изобретение позвол ет увсгли лить ч гвствительность определени лактата и снизить его предел обнару кени .The present invention allows the sensitivity of lactate to be detected and reduces its detection limit.
25 ф о р м у J а25 f o rm y J
б р еb e
тени the shadows
Способ определени концентрации лактата, включающий электролиз ис- следуемсго раствора при 20-25 С с введением в него цитохромом Вд, в фосфатном буферном растворе рН 7,0- 7.,5, содержащем медиатор, измерение тока, по которому определ ют кок- Ц€;нтрацию л:актата9 отличающийс TeMj что, с целью увели-, чени чувствительности способа, электролиз провод т в присутствии лактатдегидрогеназы, вз той в коли- че.стве 10-120 ед/1уш, к восстановленного никотинамидадениндинуклеотида в Концентрации ,0 мМ с последующим измерением тока относительно Ag//u5Cl электрода,, The method of determining the concentration of lactate, which includes electrolysis of the test solution at 20–25 ° C with the introduction of cytochrome Vd in it, in a phosphate buffer solution pH 7.0-7., 5, containing a mediator, measurement of the current, which is determined by €; the calculation of l: actat9 differs in TeMj that, in order to increase the sensitivity of the method, the electrolysis is carried out in the presence of lactate dehydrogenase, taken in a quantity of 10-120 units / 1ush, to the reduced nicotinamide adenine dinucleotide in Concentration, 0 mM s subsequent measurement of the current relative to the Ag // u5Cl electrode,
Claims (1)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU843727803A SU1242803A1 (en) | 1984-02-29 | 1984-02-29 | Method of determining lactate concentration |
| LTRP1159A LT2317B (en) | 1984-02-29 | 1993-09-27 | THE FUNCTION OF DETERMINING THE LACTATE CONCENTRATION |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU843727803A SU1242803A1 (en) | 1984-02-29 | 1984-02-29 | Method of determining lactate concentration |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU1242803A1 true SU1242803A1 (en) | 1986-07-07 |
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ID=21114044
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU843727803A SU1242803A1 (en) | 1984-02-29 | 1984-02-29 | Method of determining lactate concentration |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU1242803A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| LT3750B (en) | 1994-01-06 | 1996-03-25 | Biochemijos Institutas | Method for making of fermentation electrode for determinating of l-lactate |
-
1984
- 1984-02-29 SU SU843727803A patent/SU1242803A1/en active
Non-Patent Citations (1)
| Title |
|---|
| Methods of Enzymatic Analyses, ed Ъу Н. и. Bergmeyer assisted Ъу. К. Gavehn, Academie Press. Inc. New Jork, London,. 1974, v 3, p. 1483-1489. Патент US № 4129479, кл. G 01 N 27/46, опублик. 1981. * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| LT3750B (en) | 1994-01-06 | 1996-03-25 | Biochemijos Institutas | Method for making of fermentation electrode for determinating of l-lactate |
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