SU1224723A1 - Method of determining toxicity of polymer material - Google Patents
Method of determining toxicity of polymer material Download PDFInfo
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- SU1224723A1 SU1224723A1 SU833612471A SU3612471A SU1224723A1 SU 1224723 A1 SU1224723 A1 SU 1224723A1 SU 833612471 A SU833612471 A SU 833612471A SU 3612471 A SU3612471 A SU 3612471A SU 1224723 A1 SU1224723 A1 SU 1224723A1
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- time
- toxicity
- polymer material
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Description
Изобретение относитс к биологии и медицине, а именно к определению токсичности полимерных материалов.The invention relates to biology and medicine, in particular to the determination of the toxicity of polymeric materials.
Цель изобретени - повьппение точности способа определени токсичности полимерных материалов.The purpose of the invention is to improve the accuracy of the method for determining the toxicity of polymeric materials.
Пример 1, Испытанию на токсичность была подвергнута выт жка из системы дл переливани крови, разные детали которой изготовлены из полимерных материалов (поливинипхло- ридного пластика, медицинской резины и капронового полотна). Выт жку готовили по известной методике.Example 1 A toxicity test was drawn from a blood transfusion system, the various parts of which were made of polymeric materials (polyvinyl chloride plastic, medical rubber and nylon cloth). Wash was prepared by a known method.
Дл определени степени токсичности выт жку сравнивали с контрольным раствором. Контрольной пробой служила глюкозоцитратна среда (глюкоза 4 г, цитрат натри 0,58 г, вода дистиллированна 100 мл)оTo determine the degree of toxicity, the draws were compared with the control solution. The control sample was glucose citrate medium (glucose 4 g, sodium citrate 0.58 g, distilled water 100 ml) o
Опытным образцом в эксперименте служила выт жка, доведенна до изотонии . На 100 мл, выт жки добавл ли 4 г глюкозы и 0,58 г цитрата натри The prototype in the experiment served as stretching, reduced to isotony. In 100 ml of the extract, 4 g of glucose and 0.58 g of sodium citrate were added.
В качестве клеточного тест-объекта определени токсичности выт жки использовали замороженную в парах жидкого азота гранулированную сперму быка.The granulated bovine sperm frozen in liquid nitrogen vapor was used as a cell test object for determining the toxicity of the draw.
Оттаивание каждой гранулы осуществл ли в 0,3 мл глюкозоцитратной среды при 40°Со После оттаивани сперму сливали в колбочку и тщательно перемешивали. Затем в 1 мл опытной пробы добавл ли 0,3 мл отта нной спермы. Аналогично поступали и с контрольной пробойоThawing of each granule was carried out in 0.3 ml of a glucose-citrate medium at 40 ° C. After thawing, the semen was poured into a flask and thoroughly mixed. Then, 1 ml of the test sample was added with 0.3 ml of thawed sperm. Similarly, did the control breakdown
Определение токсичности выт жки осуществл ли с помощью контрольной установки. Приготовленные опытный и контрольный образцы помещали в измерительные кюветы. Проводили измерение произведени концентрации подвижных клеток на среднюю скорость их движени в условных единицах в динамике с интервалом 5 мин. Результаты измерени приведены вDetermination of the toxicity of the draw was carried out using a control unit. Prepared experimental and control samples were placed in the measuring cuvette. Measurements were made of the product of the concentration of motile cells by the average speed of their movement in arbitrary units over a period of 5 minutes. The measurement results are given in
табл. 1.tab. one.
ТаблицаTable
Продолжение табл.Continued table.
t. минt. min
Произведение концентрации клеток на скоростьThe product of cell concentration by speed
Опытный образецPrototype
SS
00
5five
00
5five
5five
3 4 5 6 7 8 93 4 5 6 7 8 9
10 11 12 13 14 15 16 1710 11 12 13 14 15 16 17
0 18 190 18 19
2020
2121
2222
2323
2424
2525
2626
2727
11 1611 16
21 26 31 3621 26 31 36
4141
4646
5151
5656
6161
6666
7171
7676
8181
8686
9191
9696
101101
106106
111111
116116
121121
126126
131131
104 124 100104 124 100
120120
9191
9090
9191
7676
8181
7777
6767
7474
7575
72 84 52 58 53 40 52 42 42 33 25 1972 84 52 58 53 40 52 42 42 33 25 19
98 11.7 105 111 76 74 94 ,60 55 70 49 48 22 2598 11.7 105 111 76 74 94, 60 55 70 49 48 22 25
8eight
1313
33
5five
1 .101.10
ОABOUT
о оoh oh
о . оabout . about
S1224723S1224723
Продолжение табл. 2Continued table. 2
Данные таблицы подставлены в форData tables are substituted in the odds
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU833612471A SU1224723A1 (en) | 1983-06-28 | 1983-06-28 | Method of determining toxicity of polymer material |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU833612471A SU1224723A1 (en) | 1983-06-28 | 1983-06-28 | Method of determining toxicity of polymer material |
Publications (1)
Publication Number | Publication Date |
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SU1224723A1 true SU1224723A1 (en) | 1986-04-15 |
Family
ID=21070977
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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SU833612471A SU1224723A1 (en) | 1983-06-28 | 1983-06-28 | Method of determining toxicity of polymer material |
Country Status (1)
Country | Link |
---|---|
SU (1) | SU1224723A1 (en) |
-
1983
- 1983-06-28 SU SU833612471A patent/SU1224723A1/en active
Non-Patent Citations (1)
Title |
---|
Paluch Toxicity of plastics materials as evaluated by means of bull semen Polish. I. of pharmacology and pharmacy, 1980, 32, 109-114. * |
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