SU1224723A1 - Method of determining toxicity of polymer material - Google Patents

Method of determining toxicity of polymer material Download PDF

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Publication number
SU1224723A1
SU1224723A1 SU833612471A SU3612471A SU1224723A1 SU 1224723 A1 SU1224723 A1 SU 1224723A1 SU 833612471 A SU833612471 A SU 833612471A SU 3612471 A SU3612471 A SU 3612471A SU 1224723 A1 SU1224723 A1 SU 1224723A1
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SU
USSR - Soviet Union
Prior art keywords
time
toxicity
polymer material
speed
suspension
Prior art date
Application number
SU833612471A
Other languages
Russian (ru)
Inventor
Андрей Павлович Еськов
Олег Маратович Гурилев
Александр Сергеевич Лужецкий
Юрий Николаевич Рязанов
Ренат Измайлович Каюмов
Original Assignee
Всесоюзный научно-исследовательский и испытательный институт медицинской техники
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Priority to SU833612471A priority Critical patent/SU1224723A1/en
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Publication of SU1224723A1 publication Critical patent/SU1224723A1/en

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Description

Изобретение относитс  к биологии и медицине, а именно к определению токсичности полимерных материалов.The invention relates to biology and medicine, in particular to the determination of the toxicity of polymeric materials.

Цель изобретени  - повьппение точности способа определени  токсичности полимерных материалов.The purpose of the invention is to improve the accuracy of the method for determining the toxicity of polymeric materials.

Пример 1, Испытанию на токсичность была подвергнута выт жка из системы дл  переливани  крови, разные детали которой изготовлены из полимерных материалов (поливинипхло- ридного пластика, медицинской резины и капронового полотна). Выт жку готовили по известной методике.Example 1 A toxicity test was drawn from a blood transfusion system, the various parts of which were made of polymeric materials (polyvinyl chloride plastic, medical rubber and nylon cloth). Wash was prepared by a known method.

Дл  определени  степени токсичности выт жку сравнивали с контрольным раствором. Контрольной пробой служила глюкозоцитратна  среда (глюкоза 4 г, цитрат натри  0,58 г, вода дистиллированна  100 мл)оTo determine the degree of toxicity, the draws were compared with the control solution. The control sample was glucose citrate medium (glucose 4 g, sodium citrate 0.58 g, distilled water 100 ml) o

Опытным образцом в эксперименте служила выт жка, доведенна  до изотонии . На 100 мл, выт жки добавл ли 4 г глюкозы и 0,58 г цитрата натри The prototype in the experiment served as stretching, reduced to isotony. In 100 ml of the extract, 4 g of glucose and 0.58 g of sodium citrate were added.

В качестве клеточного тест-объекта определени  токсичности выт жки использовали замороженную в парах жидкого азота гранулированную сперму быка.The granulated bovine sperm frozen in liquid nitrogen vapor was used as a cell test object for determining the toxicity of the draw.

Оттаивание каждой гранулы осуществл ли в 0,3 мл глюкозоцитратной среды при 40°Со После оттаивани  сперму сливали в колбочку и тщательно перемешивали. Затем в 1 мл опытной пробы добавл ли 0,3 мл отта нной спермы. Аналогично поступали и с контрольной пробойоThawing of each granule was carried out in 0.3 ml of a glucose-citrate medium at 40 ° C. After thawing, the semen was poured into a flask and thoroughly mixed. Then, 1 ml of the test sample was added with 0.3 ml of thawed sperm. Similarly, did the control breakdown

Определение токсичности выт жки осуществл ли с помощью контрольной установки. Приготовленные опытный и контрольный образцы помещали в измерительные кюветы. Проводили измерение произведени  концентрации подвижных клеток на среднюю скорость их движени  в условных единицах в динамике с интервалом 5 мин. Результаты измерени  приведены вDetermination of the toxicity of the draw was carried out using a control unit. Prepared experimental and control samples were placed in the measuring cuvette. Measurements were made of the product of the concentration of motile cells by the average speed of their movement in arbitrary units over a period of 5 minutes. The measurement results are given in

табл. 1.tab. one.

ТаблицаTable

Продолжение табл.Continued table.

t. минt. min

Произведение концентрации клеток на скоростьThe product of cell concentration by speed

Опытный образецPrototype

SS

00

5five

00

5five

5five

3 4 5 6 7 8 93 4 5 6 7 8 9

10 11 12 13 14 15 16 1710 11 12 13 14 15 16 17

0 18 190 18 19

2020

2121

2222

2323

2424

2525

2626

2727

11 1611 16

21 26 31 3621 26 31 36

4141

4646

5151

5656

6161

6666

7171

7676

8181

8686

9191

9696

101101

106106

111111

116116

121121

126126

131131

104 124 100104 124 100

120120

9191

9090

9191

7676

8181

7777

6767

7474

7575

72 84 52 58 53 40 52 42 42 33 25 1972 84 52 58 53 40 52 42 42 33 25 19

98 11.7 105 111 76 74 94 ,60 55 70 49 48 22 2598 11.7 105 111 76 74 94, 60 55 70 49 48 22 25

8eight

1313

33

5five

1 .101.10

ОABOUT

о оoh oh

о . оabout . about

S1224723S1224723

Продолжение табл. 2Continued table. 2

Данные таблицы подставлены в форData tables are substituted in the odds

Claims (1)

СПОСОБ ОПРЕДЕЛЕНИЯ ТОКСИЧНОО ТИ ПОЛИМЕРНОГО МАТЕРИАЛА путем приготовления водной вытяжки исследуемого полимера, воздействия этим экстрактом на суспензию подвижных клеток с последующей оценкой жизнедеятельности клеток, отличающийся тем, что, с целью повышения точности способа, одновременно определяют концентрацию подвижных клеток и скорость их движения в динамике, далее вычисляют среднее время жизни по формулеMETHOD FOR DETERMINING TOXIC POLYMERIC MATERIAL by preparing an aqueous extract of the studied polymer, exposing this suspension to a suspension of motile cells with a subsequent assessment of cell activity, characterized in that, in order to improve the accuracy of the method, the concentration of motile cells and their speed in dynamics are simultaneously determined, then calculate the average life time by the formula ч. = +-где t - среднее время жизни;hours = + -where t is the average life time; С( - концетрация клеток в данный момент времени;C ( - cell concentration at a given point in time; V; - скорость движения клеток в данный момент времени;V; - the speed of movement of cells at a given time; t - время от канала исследования до момента последующего измерения;t is the time from the research channel to the moment of subsequent measurement; I “ 1, 2, 3, .. · η , и при снижении среднего времени жизни на 10Х и более в сравнении с конт· рольным определяют токсичность полимерного материала.I “1, 2, 3, ...
SU833612471A 1983-06-28 1983-06-28 Method of determining toxicity of polymer material SU1224723A1 (en)

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SU833612471A SU1224723A1 (en) 1983-06-28 1983-06-28 Method of determining toxicity of polymer material

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Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Paluch Toxicity of plastics materials as evaluated by means of bull semen Polish. I. of pharmacology and pharmacy, 1980, 32, 109-114. *

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