SU1220644A1 - Chamber for quantitative account of nematode larvae - Google Patents
Chamber for quantitative account of nematode larvae Download PDFInfo
- Publication number
- SU1220644A1 SU1220644A1 SU813363970A SU3363970A SU1220644A1 SU 1220644 A1 SU1220644 A1 SU 1220644A1 SU 813363970 A SU813363970 A SU 813363970A SU 3363970 A SU3363970 A SU 3363970A SU 1220644 A1 SU1220644 A1 SU 1220644A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- chamber
- camera
- larvae
- nematode larvae
- nematode
- Prior art date
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- Agricultural Chemicals And Associated Chemicals (AREA)
Description
Изобретение относитс к гельминтологии, а именно к способу диагностики гельминтоза .This invention relates to helminthology, and specifically to a method for diagnosing helminthiasis.
Цель изобретени - повышение точности и сокращение времени учета личинок нематод .The purpose of the invention is to improve the accuracy and reduce the time accounting for nematode larvae.
На чертеже изображена камера дл количественного учета личинок нематод, общийThe drawing shows a chamber for quantitative accounting of nematode larvae, a common
вид.view.
Камера 1 выполнена из плексигласа толщиной 0,2 мм в виде пр моугольной емкости размерами 11X8X1,5 см. На дне камеры нанесена счетна сетка (не показана). Камера разделена перегородками 2 на каналы шириной 0,7 см, что соответствует диаметру пол зрени бинокул рного стереоскопического микроскопа (МБС-1) при кратности увеличени 2X8. Отношение высоты перегородок к высоте наружной стенки камеры равно 1:3, а соотношение ширины каналов к длине камеры 1:16.Chamber 1 is made of 0.2 mm thick plexiglass in the form of a rectangular container with dimensions 11X8X1.5 cm. At the bottom of the chamber there is a counting grid (not shown). The camera is divided by partitions 2 into channels 0.7 cm wide, which corresponds to the diameter of the field of view of a binocular stereoscopic microscope (MBS-1) at a magnification of 2X8. The ratio of the height of the partitions to the height of the outer wall of the chamber is 1: 3, and the ratio of the width of the channels to the length of the chamber is 1:16.
На наружной поверхности дна камеры 1 наклеены полоски 3 из плексигласа толщиной 0,5 мм и шириной 2 мм дл предотвращени образовани царапин при переOn the outer surface of the bottom of chamber 1, strips of 3 Plexiglas 0.5 mm thick and 2 mm wide are glued to prevent scratching
мещении камеры на предметном столике микроскопа.The camera is placed on the stage of the microscope.
Камера работает следующим образом.The camera works as follows.
Материал дл исследовани , полученный после центрифугировани или отстаивани , равномерно разливают по каналам камеры. Затем Е-; камеру добавл ют водопроводную воду комнатной температуры до тех пор, пока верхние кра каналов ее покроютс слоем воды толщиной 1-2 мм. Объем разбавленного материала равен 55 мл, что способствует обнаружению личинок даже при их незначительном содержании. Толщина жидкого сло 0,6-0,7 см позвол ет хорошо просматривать его, считать и изучать морфологию личинок. Личинки быстро оседают на дно камеры, что исключает возможность их перехода из одного канала в другой.The test material obtained after centrifuging or settling is evenly distributed through the channels of the chamber. Then E-; The tap water is added at room temperature until the upper edges of the channels are covered with a layer of water 1-2 mm thick. The volume of the diluted material is 55 ml, which facilitates the detection of the larvae even with their low content. The thickness of the liquid layer of 0.6-0.7 cm allows a good view of it, count and study the morphology of the larvae. The larvae quickly settle to the bottom of the chamber, which excludes the possibility of their transition from one channel to another.
Камеру обеззараживают путем погружени ее в 2%-ный раствор карбаювой кислоты на 15-20 ч.The chamber is disinfected by immersing it in a 2% solution of carboxylic acid for 15–20 h.
При использовании предлагаемой камеры вы вл етс на 30% больше личинок нематод и затрачиваетс на микроскопирование одной пробы в среднем в 2,7 раза меньше времени, чем при использовании Музыковского.When using the proposed camera, 30% more nematode larvae are detected and it takes an average of 2.7 times less microscopy of one sample 2.7 times less than using Muzykovsky.
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Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU813363970A SU1220644A1 (en) | 1981-12-07 | 1981-12-07 | Chamber for quantitative account of nematode larvae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU813363970A SU1220644A1 (en) | 1981-12-07 | 1981-12-07 | Chamber for quantitative account of nematode larvae |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU1220644A1 true SU1220644A1 (en) | 1986-03-30 |
Family
ID=20986003
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU813363970A SU1220644A1 (en) | 1981-12-07 | 1981-12-07 | Chamber for quantitative account of nematode larvae |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU1220644A1 (en) |
-
1981
- 1981-12-07 SU SU813363970A patent/SU1220644A1/en active
Non-Patent Citations (1)
| Title |
|---|
| Медицинска паразитологи и паразитные болезни. М.; Медицина, 1963, № 2, с. 224. * |
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