SU1217479A1 - Method of preparing fat and acid collector of biologic origin for flotation - Google Patents

Description

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This invention relates to a mineral processing technique and is widely used in mining and processing enterprises, in particular in flotation plants.

The purpose of the invention is to increase collective ability.

The essence of the method of preparing the fat-acid collector of biological origin for flotation is the use of pure culture of the mold fungus Penicillium expansum VKM F-698, which is capable of dehydrogenating the reagent fats.

Characteristic of Penicillium expansum VKM F - 698.

This culture was obtained from the All-Union Collection of Microorganisms. This strain is derived from the Ukrainian Institute of Food Industry from food products. Previously named Penicilliun glaucum,

The fungus belongs to imperfect fungi, since members of the genus Penicillium do not form higher marsupial sporulation. Penicilli and Aspergillus constitute one of the most important and common groups of European fungi. In contrast to the type strain, P. expansum F - 698 conidiophores are solitary, they do not express bundles of the coremia type. Mycelium is colorless, sometimes darkening in old age.

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Colonies of Penicillium exrapic VKM F - 698 are gray-gray or gray-green from above. Developed on fruit during storage. The eonidia are first elliptical, then spherical, 3-3.4 microns in diameter, p. expansum VKM F. - 698 grows well and spores on ordinary nutrient media: potato agar, potato-carrot agar, on апapek’s medium, on wort 3 ° B. On solid media, the image goes gray velvety colonies. From typical P, expansum differs in that it forms a yellow exudate diffusing into the medium.

The fungus has the ability to dehydrogenate fats, as a result of this process, the saturated fatty acids become unsaturated, which can be used for flotation. As a substrate for the production of unsaturated fatty acids

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fatty acid bioreagents can serve.

The culture is well preserved in a lyophilized state, under oil and in soil.

The method is carried out as follows.

The biomass of the biologically derived reagent (microblast fat, jj of the fungus Pelakeslea trispore - 490, lipids of the yeast Lipamyces Lipoferus - 199, lipids of Chlorophyta algae placed in a plant chamber with vertical channels for air supply heated to 30, culture of mold fungus - grows - ryan - 76 expansum VKM F - 698. The presence of vitamins and physiologically active substances in biologically derived reagents gives an opportunity to

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It is possible to significantly increase the viability and active activity of the fungus. Hydrolytic activity of life cells of the fungus and their method 2,. The ability to release exolipase into a culture fluid (with the hydrolysis of the ester linkage with stearic acid being possible, preferably) increases the saturation of the contained 1 fatty acids.

After three days of cultivation of the fungus (without feeding the nutrients) on the biomass of the reagents, the resulting product was analyzed for the determination of the iodine number and not flotation as a collector.

The resulting biomass of reagents has a more liquid consistency and easily forms a stable emulsion, without the addition of alkali or oleic acid.

 PRI me R. B capacity, having vertical channels for transmission of air heated to 30 °, space. bioreagents and sterile water. Bioreagents are infected with the culture of the fungus Penicillium exprapvlp - 698, which is grown for three days. Bioreagents treated with this method are used for flotation of fluorite in the amount of

 1.25 mg / l, in parallel flotation is carried out with the same amount of untreated reagents. The results are presented in the table.

During flotation of fluorite-containing

 ORE microbial fat processed by the fungus in the concentrate passed 1.7% more fluorite with a decrease in 3 times the consumption of oleic acid.

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Fluorite extraction,%

Bioreagents treated with fungus Bioreagents without treatment

Penicillimn expansum - 698

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Microbial Fat 78jO51.1

Fungus Lipids 84,447,7

Algae lipids 88,049,1

Claims (1)

METHOD FOR PREPARING A FATTY ACID GATHER OF BIOLOGICAL ORIGIN FOR FLOTATION, which involves treating the biomass of the initial reagent, characterized in that, in order to increase the collective ability, the biomass of the initial reagent is treated with a pure culture of the fungus Penicillium expansum fatty acid reagent 69 GO • m m s