SU1217302A1 - Method of determining cold-resistance of plants - Google Patents

Description

The invention relates to the selection of plants and can be used to assess the cold resistance of plants.

The purpose of the invention is to increase the accuracy of determination.

The method is carried out as follows.

From the middle part of the same-oriented shoots, the leaves are harvested, immediately taken to the laboratory and placed on the stage of the spectroscope. A sheet of monochromatic light is projected onto a sheet. The part of the light reflected by the surface of the sheet is extinguished by the light filter and the excited luminescent glow through the second monochromator enters the photomultiplier. The signal amplified by the device is recorded by a recording potentiometer.

After the luminescence intensity is stabilized in the illuminated sheet (Kutsky effect), it is pulsed to cool. The sheet is cooled for 5-7 s, and its luminescent luminescence is recorded this time. After that, the cooling of the sheet is stopped, its temperature rises to the initial level within 1-2 minutes, then it is cooled again to a temperature 1-2 C below

previous cooling temperatures and record fluorescent fluorescence.

Sequentially cooling the leaves of the studied plants, fix the temperature at which the value of the peak reaches a maximum and with a subsequent decrease remains constant.

Diagnostics of plant cold resistance are carried out on a fluorescent spectrometer of the following design: to excite the luminescence of the leaves of the plants, a 1 kW water-cooled xenon lamp is used. The polychromatic light of a lamp passes through a monochromator, which from the common light spectrum separates a band of light with a wavelength of 485 nm and with the help of

VERSION

Order 1019/2

Branch PIP Patent, Uzhgorod, st. Project, 4

lenses designs on a sheet of plants.

The diameter of the light beam is 4-5 mm. The light reflected by the surface of the sheet is quenched by a KS-11 type optical filter, and the luminescent glow emitted by the photosynthetic complex passes unhindered through the light filter and enters the second monochromator. The function of the second monochromator is to isolate a band of light with a wavelength of 692 nm and design it on a photoelectron multiplier (PMT).

Example .. In determining the cold resistance of peach plants, leaves that were picked from the middle part of the same-oriented shoots in the morning and stylized-. To avoid loss of moisture, the leaves are placed in plastic bags and delivered to the laboratory.

Freshly picked leaves are alternately placed in cassettes and placed on an object table of the spectrometer. Then on the leaves design, a beam of light and oriented to the window of the monochromator so that the amplifying device gives the maximum performance.

At the beginning of the illumination, the luminescence intensity decreases rapidly and then gradually stabilizes. After the luminescent light reaches the stationary level by blowing off with cooled air or by contacting a microcooler, the sheet is subjected to short-term cooling. With sufficiently intensive cooling, the sheet temperature decreases to the specified parameters in 5–7 s. At this time, the recorder records the intensity of the fluorescent light.

After cooling for 1-2 minutes, the sheet is heated to the initial temperature. In the subsequent cooling cycle, the temperature is reduced by 1-2 ° C. At. this is up to a certain limit and the intensity of the luminescence increases. After a certain number of cooling cycles, the intensity of the luminescent sheet reaches the maximum level and does not increase further.

Circulation 679

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Claims (1)

METHOD FOR DETERMINING COLD RESISTANCE OF PLANTS by recording the intensity of ultra-weak luminescence of leaves at the initial temperature and after cooling and subsequent analysis of the obtained data, characterized in that, in order to improve the accuracy of determination, the sheet is cooled in steps with a gradient of 1-2 ° C for 5-7 second pulses with subsequent 1-2-minute breaks at the initial temperature, the flash intensity of the luminescent glow after each cooling is recorded, and temperatures are considered an indicator of cold resistance At which the flash amount reaches a maximum, and the subsequent reduction remains constant.