SU1181668A1 - Method of cleaning blood plasma from pathological protein complexes - Google Patents

Abstract

A METHOD FOR CLEANING BLOOD PLASMA FROM PATHOLOGIC PROTEIN. COMPLEX, including plasmapheresis, characterized in that, in order to increase the excretion of pathological protein complexes, heparin is added to the plasma separated by plasmapheresis. 7-10 thousand U / l incubated for 5-18 hours at which time the precipitate is separated, centrifuged, and the resulting supernatant is frozen at -20 to -35 ° C and thawed, followed by centrifugation of S. S (L C

Description

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about

ABOUT)

00 11 The invention relates to medicine in particular to methods of treating conditions associated with immunocomplex pathology, cryo-lobulinemia, cryofibrinogenemia, disseminated intravascular coagulation syndrome, massive cell decay, i.e. conditions associated with the pulsation of pathological protein complexes in plasma. The gap of the invention is to increase the introduction of pathological protein complexes from the blood plasma. PR JJ, meper 1. After puncture of the ulnar vein, blood was expelled into one of the paired plasticine containers Gemakon 500-300, containing 100 ml of hemoconservative Glugicir. At the end of the exfusion of 400 MP of blood, the container was disconnected and lowered into the K-70 refrigeration centrifuge (GDR) chamber. A physiological solution was injected into the vein during the rifting center. Centrifugation was performed at a speed of 1500 rpm at 22 ° C for 20 minutes. During centrifugation, the blood was divided into blood cells and plasma. The latter was transferred to the second container with the help of plasma extractor, and the uniform elements were returned to the patient, after having previously suspended them in 50 ml of physiological solution. After the return of the form elements was completed, the following blood exhalation was performed. In just one plasmaphoresis procedure, 4 to 6 exams were performed. In one procedure in plastic containers collected 1500 ml of plasma. Heated sterilized plasma plasma containers were used to administer heparin from a preparation of 10.00 U per liter. The plasma was incubated for 18 h at 4 ° C. The fibronectin precipitate formed was separated from the plasma by centrifuging at a speed of 3500 rpm at 4 ° C for 20 minutes and the supernatant was transferred to another container under sterile conditions and immediately frozen at 20 ° C. The supernatant was stored for no more than 1 week. the plae mophoresis supernatant was thawed, centrifuged at a speed of 3500 rpm at 4 ° C for 15 minutes, the rest of the cryobelks were removed and used to replace the plasma loss resulting from repeated plasma exchange 8 Plasma substitutes and preparations rovi not been used. Returned 70-80% of autoplasma. control crops autoplasma be sterile. According to the described method, 5 selective plasmapheresis was performed with a frequency of 2 times a week. The results obtained in the treatment of selective removal of plasma fibronectin, are presented in Table 1; Thus, as a result of treatment by selective removal of plasma fibronectin in a patient of 3, 45 years, a clear positive therapeutic effect was achieved: the clinical manifestations of the disease disappeared, the laboratory indices almost normalized. It should be noted that prior therapy, including disaggregants, non-hormonal anti-inflammatory drugs, direct anticoagulants, was not effective. Example 2, Treatment of immunocomplex pathology and cryoglobulinemia of the patient, 64 years old. Diagnosis: Sjogren's syndrome, Idiopathic cryoglobulinema, Thrombohemorrhagic vasculitis. Polyneuritis. Reine syndrome. Plasma phoresis was performed according to the procedure described in Example 1, 2. Incubation with heparin was carried out for 12 hours at 6 ° C. The formed fibronectin precipitate with immune complexes, cryoglobulins were separated from the plasma by centrifugation at 3000 rpm at 6 ° C. 15 min, and the supernatant was transferred to another container and immediately frozen at -35 ° C. The storage supernatant was as shown in Example 1. Before re-plasmapheresis, the supernatant was thawed, centrifuged at 3000 rpm at 4 ° C for 10 minutes, removing the remaining cryobels and used to replenish plasmapheresis. Plasma substitutes and patient blood products were not used. The number of recovered autoplasma and the results of its inoculation are similar to Example 1. The described method allowed plasmaphoresis to be carried out daily. No complications. The results obtained in the treatment of selective removal from plasma of ibronectin, immune complexes. 3 cryoglobulins, are presented in table. 2. As a result of the treatment, a distinct clinical and laboratory effect was obtained. Intensive plasmapheresis (10 procedures were performed) in patient V., 64 years old, according to the proposed method did not lead to a decrease in total protein in the blood. Example Extremely treated patient E., 37 years old. Diagnosis: lymphoproliferative syndrome with the production of RU. Cryoglobulinemia. Hypervia syndrome. The patient was started on a course of VAMP therapy, against the background of which agranulocytosis, thrombocytopenia, and disseminated intravascular coagulation developed. At this time, rashes, characteristic of both vasculitis and thrombocytopenic hemorrhage, appeared all over the body, hemorrhagic syndrome (uterine, epistaxis) intensified, increased sharply, and the tachycardia increased. In plasma at room temperature, a large amount of cryofibrin-Tnene was formed, cryo-clinical and laboratory parameters were precipitated

Vyshani on shin x Abdominal pain Swelling of the joints Microgematuri Proteinuri,% ESR, min / h

Sialic acid, IU CRP

Clinical and laboratory Pre-treatment indicators

Hemorrhagic vyshani

Generalized Joint Pain

After treatment

Before treatment

Not

7

240

1 (+)

Table 2 After treatment

No Does not mean 684 globulins. According to vital indications, the therapy was started by the proposed method. Plasmaphoresis was carried out according to the procedure described in Example 1. Heparin was incubated for 5 hours at 4 ° C. All subsequent steps of the method were carried out as in example 1. During the first plasmapheresis, platelet mass was transfused. After the separation of fibronectin with the accompanying products of the degradation of fibrinogen, cryoglobulins and cryofibrinogen, thawed autoplasma was returned to the patient at the time of the re-plasmapheresis. The results obtained in the treatment of an extreme condition by the selective removal from plasma of fibronectin, products of degradation of fibrinogen, cryoglobulins, cryofibrinogen, are presented in Table. 3. Thanks to the proposed method, the patient was brought out of an extreme state, which allowed later on to carry out the planned polychemistry of its aliyu. Table 1

Clinical and labolatornye.

Before treatment, indicators

Expressed

in the fingers

Distinct

There is a p

Positive

58

40

400

ED

4 / + /

. Continuation of Table 2 After treatment No

Not

Not determined

Negative

58.3

13

170

Negative

Claims (1)

METHOD FOR CLEANING BLOOD PLASMA FROM PATHOLOGICAL PROTEINS. COMPLEXES, including carrying out plasmapheresis, characterized in that, in order to increase the excretion of pathological protein complexes, the heparin is added to the plasma separated by plasmapheresis. 7-10 thousand U / L incubated for 5-18 hours at 4 ~ 6 ° C, the precipitate formed is separated by centrifugation, and the obtained supernatant is frozen at a temperature of -20 to -35 ° C and thawed, followed by centrifugation * _β <g (Λ с> 1181668 2