SU1165994A1 - Method of determining osmotic resistance of erythrocytes - Google Patents
Method of determining osmotic resistance of erythrocytes Download PDFInfo
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- SU1165994A1 SU1165994A1 SU833611715A SU3611715A SU1165994A1 SU 1165994 A1 SU1165994 A1 SU 1165994A1 SU 833611715 A SU833611715 A SU 833611715A SU 3611715 A SU3611715 A SU 3611715A SU 1165994 A1 SU1165994 A1 SU 1165994A1
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- erythrocytes
- osmotic resistance
- sodium chloride
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Abstract
СПОСОБ ОПРЕДЕЛЕНИЯ ОСМОТИЧЕСКОЙ РЕЗИСТЕНТНОСТИ ЭРИТР01ЩТОВ путем воздействи на пробу гипотоническим раствором хлорида натри , отличающийс тем, что, с ускорени повьвпенй чувствительиостии обеспечени возможности осущест ,влеии способа в полевых услови х пробу крови нанос т наширокопористую подложку, погружают ее в раствор хлорида натри , выдерживают подложку до полного ее смачивани и при наличии п тна крови определ ют осмотическую резистентность эритроцитов .METHOD FOR DETERMINING osmotic resistance ERITR01SCHTOV by subjecting the sample hypotonic solution of sodium chloride, characterized in that, on accelerating povvpeny chuvstviteliostii permit osuschest, vleii method in field conditions, the blood sample is applied nashirokoporistuyu substrate is dipped it in the sodium chloride solution is heated substrate to its full wetting and in the presence of a blood spot, determine the osmotic resistance of erythrocytes.
Description
ОABOUT
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Изобретение относитс к медицине точнее к биофизическим способам дианостики в гематологии.This invention relates to medicine, more precisely, to biophysical methods for dianostik in hematology.
ЦеАь изобретени ускорение, повышение чувствительности и обеспечение возможности осуществлени способа в полевых услови х.The intent of the invention is to accelerate, increase sensitivity and make it possible to carry out the method in the field.
Изобретение осуществл ют следующим образом.The invention is carried out as follows.
М гкую широкопористую бумагу с предварительно нанесенными на нее эритроцитами погружают в растворыхлорида натри в различной концентрации . При движении жидкости череэ п тно крови, нанесенной нафильтр, происходит вымывание свободногоSoft, wide-pored paper with red blood cells previously deposited on it is immersed in sodium chloride solution in varying concentrations. When the fluid moves through the bloodstream of the blood applied to the filter, the free
гемоглобина из гемолизированных клеток и обесцвечивани п тна. Причем степень обесцвечивани п тен пропорциональна доле гемолизированных зритроцйтов. Таким образом, визуально можно оценить осмотическую резистнвйость красных клеток крови. Дл исследовани у пациента берут кровь из пальца (около 50 мкл), нанос т на полоску фильтровальной бумаги затем помещают в лунку с раствором хлорида 11атри разной концентрации. О поступлении гемолиза суд т по исчезновению п тна крови ца бумаге при полном смачивании Полости бумаги.hemoglobin from hemolyzed cells and spot discoloration. Moreover, the degree of bleaching of the spot is proportional to the proportion of hemolyzed viewers. Thus, the osmotic resistance of red blood cells can be visually assessed. For the study, blood from a finger (about 50 µl) was taken from the patient, applied to a strip of filter paper, then placed in a well with a solution of 11 sodium chloride of different concentrations. The admission of hemolysis is judged by the disappearance of the spot of blood on the paper with full wetting of the paper cavity.
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Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU833611715A SU1165994A1 (en) | 1983-06-27 | 1983-06-27 | Method of determining osmotic resistance of erythrocytes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU833611715A SU1165994A1 (en) | 1983-06-27 | 1983-06-27 | Method of determining osmotic resistance of erythrocytes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU1165994A1 true SU1165994A1 (en) | 1985-07-07 |
Family
ID=21070704
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU833611715A SU1165994A1 (en) | 1983-06-27 | 1983-06-27 | Method of determining osmotic resistance of erythrocytes |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU1165994A1 (en) |
-
1983
- 1983-06-27 SU SU833611715A patent/SU1165994A1/en active
Non-Patent Citations (1)
| Title |
|---|
| Приказ МЗ СССР 960 от 15 окт бр 1974.года. * |
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