SU1124932A1 - Method of diagnosis of prostatitis - Google Patents

Abstract

METHOD OF DIAGNOSTICS OF PROSTATITIS by biochemical study of prostate gland secretion, characterized in that, in order to increase the accuracy of the method, the activity of kallikrein is determined and, if it is increased in comparison with normal, prostatitis is diagnosed.

Description

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The invention relates to medicine and will find wide application in urology for the diagnosis of prostatitis, which occupies one of the first places among urogenital pathology in men.

A known method for the diagnosis of prostatitis by biochemical studies of the prostate gland 1.

However, the method is not accurate enough, there is no strict specificity for prostatitis. Since a comprehensive assessment of five biochemical parameters is required, the method is very laborious, takes a large amount of time (12-14 hours per study).

The purpose of the invention is to improve the accuracy of the method.

This goal is achieved by the fact that, according to the method for diagnosing prostatitis by biochemical examination of the secretion of the prostate gland, aq. The effectiveness of kallikrein and with its increase in comparison with the norm diagnose prostatitis.

The method is carried out as follows.

The patient is washed with the urethra 10-15 ml of saline. In the knee-elbow position, the prostate gland is massaged, the resulting secret is placed in a siliconized tube and diluted with a physiological solution of 1:10, then the presence of components of the kallikrein-kinin system is determined in it: the total BAEE-hoterase activity of the non-adsorbed fraction, the total BAEE-esterase activity, antitryptic activity.

To determine the total BAEE-esterase activity of the non-adsorbed fraction, 0.125 ml of prostate gland secretion is taken, added to a test tube containing 0.5 ml of 0.02 M pH 7.0 phosphate buffer, and 2 ml of a thick suspension of DEAF-Sephadex A- 50. The resulting slurry is mixed quickly and gently with a glass rod. After 10 minutes, 1.5 ml of 0.02 pH 7.0 phosphate buffer was added. After another 2 minutes, the resulting non-adsorbed fraction is transferred to a filter and collected in a siliconized measuring tube, then 0.02 M pH 7.0 phosphate buffer is added to the filtrate to 2.5 ml. 1 ml of the obtained filtrate is introduced into a tube with 1 ml of Tris HC1 pH 8.0, after which 1 ml of 1.5x10 M BAEE is added and the optical density is measured on a spectrophotometer (SF-26) for 15 minutes at 253 nm against control on reagents.

To determine the total BAEE-esterase activity, 0.03 ml of prostate secretion is taken, added to a test tube with 2 ml of Tris HCII pH 8.0 and 1 ml of 1.5x10 M BAEE added to the tube. Optical density is measured for 30 minutes at

253 nm vs control for reagents on the SF-26.

To determine the antithyptic activity of prostate secretion, 0.1 ml of secretion is introduced into a test tube with 1.8 ml of tris pH 8.0, and 0.1 ml of 1% / trypsin solution is also added. After 5 minutes, I ml of 1.5 x 10 M BAEE are added and readings are taken from the spectrophotometer (SF-26).

The study of the three components of the kallikrein-kinin system in one patient takes about 40 minutes.

The presence of components of the kallikreinkinin system in the prostate secretion indicates inflammatory damage to the organ, and the higher the performance of the components, the more pronounced the inflammatory and dystrophic changes in the prostate. In healthy individuals in the prostate gland, the presence of components of the kallikrein-kinin system has not been identified. When examining 20 patients with chronic prostatitis IMI, the indicators of the kallikreinkinin system were as follows: total BAEE esterase activity of the non-adsorbed fraction from I to 100 honey / ml or more, total BAEE esterase activity from 1 to 200 ee / ml and more, antitrytic activity from 1 to 50 s / ml

Example}. Patient D., 30 years old, was admitted to the urology department with complaints of pain in the perineum, lower abdomen, cramps during urination, and general weakness. These symptoms in a patient are noted within 6 months. General clinical tests, as well as microscopic examination of the prostate secretion, do not reveal any pathology on the part of the prostate. In connection with

what targeted treatment was not carried out. The patient was tested for prostatic secretion for the presence of components of the kallikrein-kinin system. In the study it turned out that

The total BAEE esterase activity of the non-adsorbed fraction is equal to 24 med / ml, the total activity of the bio energy concentration is 67 OE / ml, the antitryptic activity is 16 IU / ml, which is increased compared to the norm. Based on the data

0 diagnosed - prostatitis. Comprehensive treatment for this disease was performed. The patient has decreased pain syndrome, dysuric disorders disappeared, work capacity has recovered.

The advantage of the proposed method in comparison with the known one is that it allows a reliable diagnosis, 1124932.

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prostatitis even in those cases where the disease. Furthermore, the method is simple,

yes, general laboratory and special pri-takes 10-12 times less time for

These do not allow you to diagnose this study.

Claims (1)

METHOD FOR DIAGNOSTIC OF PROSTATITIS by biochemical examination of the secretion of the prostate gland, characterized in that, in order to increase the accuracy of the method, the activity of kallikrein is determined and when it is increased in comparison with the norm, prostatitis is diagnosed. u with Go ye